Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging.

Deciphering cellular components and the spatial interaction network of the tumor immune microenvironment (TIME) of solid tumors is pivotal for understanding biologically relevant cross-talks and, ultimately, advancing therapies. Multiplexed tissue imaging provides a powerful tool to elucidate spatial complexity in a holistic manner. We established and cross-validated a comprehensive immunophenotyping panel comprising over 121 markers for multiplexed tissue imaging using MACSima™ imaging cyclic staining (MICS) alongside an end-to-end analysis workflow. Applying this panel and workflow to primary cancer tissues, we characterized tumor heterogeneity, investigated potential therapeutical targets, conducted in-depth profiling of cell types and states, sub-phenotyped T cells within the TIME, and scrutinized cellular neighborhoods of diverse T cell subsets. Our findings highlight the advantage of spatial profiling, revealing immunosuppressive molecular signatures of tumor-associated myeloid cells interacting with neighboring exhausted, PD1

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Copyright © 2024 Scheuermann, Kristmann, Engelmann, Nuernbergk, Scheuermann, Koloseus, Abed, Solass and Seitz.

Déclaration de conflit d'intérêts

The authors acknowledge that this work has been initiated as part of a collaborative research agreement with Miltenyi Biotec within our institution functioning as beta testers for the MACSima™ imaging platform. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.