Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform.

DNA methylation age estimation forensic genetics pyrosequencing trace sample

Journal

Forensic sciences research
ISSN: 2471-1411
Titre abrégé: Forensic Sci Res
Pays: England
ID NLM: 101724928

Informations de publication

Date de publication:
Mar 2024
Historique:
received: 17 01 2023
accepted: 26 09 2023
medline: 3 4 2024
pubmed: 3 4 2024
entrez: 3 4 2024
Statut: epublish

Résumé

Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.

Identifiants

DOI: 10.1093/fsr/owad055 PMID: 38567377 PMC: PMC10986743
pubmed: 38567377
doi: 10.1093/fsr/owad055
pii: owad055
pmc: PMC10986743
doi:

Types de publication

Journal Article

Langues

eng

Pagination

owad055

Informations de copyright

© The Author(s) 2024. Published by OUP on behalf of the Academy of Forensic Science.

Auteurs

Marie-Louise Kampmann (ML)

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.

Jan Fleckhaus (J)

Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany.

Claus Børsting (C)

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.

Helena Jurtikova (H)

Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic.

Alice Piters (A)

Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.

Julien Papin (J)

Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.

Quentin Gauthier (Q)

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Mirna Ghemrawi (M)

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Christian Doutremepuich (C)

Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.

Bruce McCord (B)

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Peter M Schneider (PM)

Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany.

Jiri Drabek (J)

Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic.

Niels Morling (N)

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.

Classifications MeSH