Structural and functional analysis of the key enzyme responsible for the degradation of ochratoxin A in the Alcaligenes genus.

Amidohydrolase and carboxypeptidase M20D enzymes Mycotoxin Ochratoxin A

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
02 Apr 2024
Historique:
received: 08 12 2023
revised: 01 03 2024
accepted: 01 04 2024
medline: 5 4 2024
pubmed: 5 4 2024
entrez: 4 4 2024
Statut: aheadofprint

Résumé

The potential to degrade ochratoxin A (OTA), a highly poisonous mycotoxin, was investigated in cultures from Alcaligenes-type strains. Genome sequence analyses from different Alcaligenes species have permitted us to demonstrate a direct, causal link between the gene coding a known N-acyl-L-amino acid amidohydrolase from A. faecalis (AfOTH) and the OTA-degrading activity of this bacterium. In agreement with this finding, we found the gene coding AfOTH in two additional species included in the Alcaligenes genus, namely, A. pakistanensis, and A. aquatilis, which also degraded OTA. Notably, A. faecalis subsp. faecalis DSM 30030

Identifiants

pubmed: 38574921
pii: S0141-8130(24)02147-0
doi: 10.1016/j.ijbiomac.2024.131342
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

131342

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no competing financial interest.

Auteurs

Ana Sánchez-Arroyo (A)

Institute of Food Science, Technology and Nutrition (ICTAN), CSIC, José Antonio Novais 6, 28040 Madrid, Spain.

Laura Plaza-Vinuesa (L)

Institute of Food Science, Technology and Nutrition (ICTAN), CSIC, José Antonio Novais 6, 28040 Madrid, Spain.

Blanca de Las Rivas (B)

Institute of Food Science, Technology and Nutrition (ICTAN), CSIC, José Antonio Novais 6, 28040 Madrid, Spain.

José Miguel Mancheño (JM)

Department of Crystallography and Structural Biology, Institute of Physical Chemistry Blas Cabrera, CSIC, Serrano 119, 28006 Madrid, Spain. Electronic address: jm.mancheno@csic.es.

Rosario Muñoz (R)

Institute of Food Science, Technology and Nutrition (ICTAN), CSIC, José Antonio Novais 6, 28040 Madrid, Spain. Electronic address: r.munoz@csic.es.

Classifications MeSH