Comparison of two serological diagnosis tests for bovine paratuberculosis.
Mycobacterium avium subsp. paratuberculosis
Bovine paratuberculosis
ELISA
Sonicated antigen
Journal
Veterinary research communications
ISSN: 1573-7446
Titre abrégé: Vet Res Commun
Pays: Switzerland
ID NLM: 8100520
Informations de publication
Date de publication:
05 Apr 2024
05 Apr 2024
Historique:
received:
25
01
2024
accepted:
25
03
2024
medline:
5
4
2024
pubmed:
5
4
2024
entrez:
4
4
2024
Statut:
aheadofprint
Résumé
Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an "in-house" ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5-9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93-0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6-0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit.
Identifiants
pubmed: 38575801
doi: 10.1007/s11259-024-10363-7
pii: 10.1007/s11259-024-10363-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1006/abio.1976.9999
doi: 10.1006/abio.1976.9999
pubmed: 942051
Collins M, Wells S, Petrini K, Collins J, Schultz R, Whitlock R (2005) Evaluation of five antibody detection tests for diagnosis of bovine paratuberculosis. Clin Vaccine Immunol 12:685–692. https://doi.org/10.1128/CDLI.12.6.685-692.2005
doi: 10.1128/CDLI.12.6.685-692.2005
Dane H, Stewart LD, Grant IR (2023) Culture of Mycobacterium avium subsp. paratuberculosis: challenges, limitations and future prospects. J Appl Microbiol 134:lxac017. https://doi.org/10.1093/jambio/lxac017
doi: 10.1093/jambio/lxac017
pubmed: 36626735
Diéguez FJ, González AM, Menéndez S, Vilar MJ, Sanjuán ML, Yus E, Arnaiz I (2009) Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. Vet J 180:231–235. https://doi.org/10.1016/j.tvjl.2007.11.004
doi: 10.1016/j.tvjl.2007.11.004
pubmed: 18314355
Faruk M, Jung Y, Hur T, Lee S, Cho Y (2020) Longitudinal study of Mycobacterium avium subsp. paratuberculosis antibody kinetics in dairy cattle using sera and milk throughout the lactation period. Vet Sci 7:81. https://doi.org/10.3390/vetsci7030081
doi: 10.3390/vetsci7030081
pubmed: 32629919
pmcid: 7560089
Gilardoni L, Paolicchi F, Mundo SL (2012) Bovine paratuberculosis: a review of advantages and disadvantages of the different diagnostic tests. Rev Argent Microbiol 44:201–215
pubmed: 23102470
Gopi B, Vir Singh R, Kumar S, Kumar S, Chauhan A, Sonwane A, Kumar A, Bharati J, Vir Singh S (2022) Effect of selected single nucleotide polymorphisms in SLC11A1, ANKRA2, IFNG and PGLYRP1 genes on host susceptibility to Mycobacterium avium subspecies paratuberculosis infection in Indian cattle. Vet Res Commun 46:209–221. https://doi.org/10.1007/s11259-021-09849-5
doi: 10.1007/s11259-021-09849-5
pubmed: 34718924
Landis JR, Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33:159–174
doi: 10.2307/2529310
pubmed: 843571
Li L, Bannantine J, Campo J, Randall A, Grohn Y, Katani R, Schilling M, Radzio-Basu J, Kapur V (2017) Identification of sero-reactive antigens for the early diagnosis of Johne’s disease in cattle. PLoS ONE 12:e0184373. https://doi.org/10.1371/journal.pone.0184373
doi: 10.1371/journal.pone.0184373
pubmed: 28863177
pmcid: 5581170
Mckenna S, Barkema H, Keefe G, Sockett D (2006) Agreement between three ELISAs for Mycobacterium avium subsp. paratuberculosis in dairy cattle. Vet Microbiol 114:285–291. https://doi.org/10.1016/j.vetmic.2005.12.002
doi: 10.1016/j.vetmic.2005.12.002
pubmed: 16442752
Nielsen SS (2020) Immune-based diagnosis of paratuberculosis. In: Behr MA, Stevenson K, Kapur V (eds) Paratuberculosis: Organism, Disease, Control, 2nd edn. CAB International, Gloucester, pp 333–345
doi: 10.1079/9781789243413.0333
Nielsen SS, Toft N (2008) Ante mortem diagnosis of paratuberculosis: a review of accuracies of ELISA, interferon-gamma assay and fecal culture techniques. Vet Microbiol 129:217–235. https://doi.org/10.1016/j.vetmic.2007.12.011
doi: 10.1016/j.vetmic.2007.12.011
pubmed: 18255239
Paolicchi F, Zumárraga M, Giofré A, Zamorano P, Morsella C, Verna A, Cataldi A, Alito A, Romano M (2003) Application of different methods for the diagnosis of paratuberculosis in dairy cattle herds in Argentina. J Vet Med 50:20–26. https://doi.org/10.1046/j.1439-0450.2003.00606.x
doi: 10.1046/j.1439-0450.2003.00606.x
Ridge SE, Morgan IR, Sockett DC, Collins MT, Condron RJ, Skilbeck NW, Webber JJ (1991) Comparison of the Johne’s absorbed EIA and the complement-fixation test for the diagnosis of Johne’s disease in cattle Aust. Vet J 68:253–257. https://doi.org/10.1111/j.1751-0813.1991.tb03230.x
doi: 10.1111/j.1751-0813.1991.tb03230.x
Singh S, Dhakal I, Singh U, Devkota B (2018) Current diagnostic techniques of Mycobacterium avium subsp. paratuberculosis in domestic ruminants. J Agric Forestry Univ 2018:23–34
Vasini B, Farace P, Ariel A, Cirone K, Mendez L, Morsella C, Fresia P, Iraola G, Gioffré A, Paolicchi F (2022) Phylogenetic and multiple–locus variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina. Vet Res Commun 46:1121–1129. https://doi.org/10.1007/s11259-022-09983-8
doi: 10.1007/s11259-022-09983-8
pubmed: 35948855
Whitlock RH, Buergelt C (1996) Preclinical and clinical manifestations of paratuberculosis (including pathology). Vet Clin North Am Food Anim Pract 12:345–356. https://doi.org/10.1016/s0749-0720(15)30410-2
doi: 10.1016/s0749-0720(15)30410-2
pubmed: 8828109
Whitlock RH, Wells SJ, Sweeney RW, Van Tiem J (2000) ELISA and fecal culture for paratuberculosis (Johne´s disease): sensitivity and specificity of each method. Vet Microbiol 77:387–398. https://doi.org/10.1016/s0378-1135(00)00324-2
doi: 10.1016/s0378-1135(00)00324-2
pubmed: 11118724
Whittington R (2020) Cultivation of Mycobacterium avium subsp. paratuberculosis. In: Behr MA, Stevenson K, Kapur V (eds) Paratuberculosis: Organism, Disease, Control, 2nd edn. CAB International, Gloucester, pp 266–304
doi: 10.1079/9781789243413.0266
Winterhoff C, Beyerbach M, Homuth M, Strutzberg K, Gerlach GF (2002) Establishment and evaluation of an ELISA for the detection of antibodies in milk against Mycobacterium avium subspecies paratuberculosis. Dtsch Tierarztl Wochenschr 109:230–234
pubmed: 12073496