Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato.
Backcross Inbred Lines
Introgression
Solanum pennellii
breeding
fine-mapping
recombination rate
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
04 Apr 2024
04 Apr 2024
Historique:
revised:
11
03
2024
received:
01
11
2023
accepted:
14
03
2024
medline:
5
4
2024
pubmed:
5
4
2024
entrez:
5
4
2024
Statut:
aheadofprint
Résumé
Wild species are an invaluable source of new traits for crop improvement. Over the years, the tomato community bred cultivated lines that carry introgressions from different species of the tomato tribe to facilitate trait discovery and mapping. The next phase in such projects is to find the genes that drive the identified phenotypes. This can be achieved by genotyping a few thousand individuals resulting in fine mapping that can potentially identify the causative gene. To couple trait discovery and fine mapping, we are presenting large, recombination-rich, Backcross Inbred Line (BIL) populations involving an unexplored accession of the wild, green-fruited species Solanum pennellii (LA5240; the 'Lost' Accession) with two modern tomato inbreds: LEA, determinate, and TOP, indeterminate. The LEA and TOP BILs are in BC2F6-8 generation and include 1400 and 500 lines, respectively. The BILs were genotyped with 5000 SPET markers, showing that in the euchromatic regions there was one recombinant every 17-18 Kb while in the heterochromatin a recombinant every 600-700 Kb (TOP and LEA respectively). To gain perspective on the topography of recombination we compared five independent members of the Self-pruning gene family with their respective neighboring genes; based on PCR markers, in all cases we found recombinants. Further mapping analysis of two known morphological mutations that segregated in the BILs (self-pruning and hairless) showed that the maximal delimited intervals were 73 Kb and 210 Kb, respectively, and included the known causative genes. The 'Lost'_BILs provide a solid framework to study traits derived from a drought-tolerant wild tomato.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Commission, Horizon 2020 program
ID : 862201 (CAPITALIZE)
Organisme : European Commission, Horizon 2020 program
ID : 677379 (G2P-SOL)
Organisme : European Commission, Horizon 2020 program
ID : 101000716 (HARNESSTOM)
Organisme : European Commission, Horizon Europe program
ID : 101094738 (PRO-GRACE)
Organisme : ISF Israel science foundation
ID : 2365/20
Informations de copyright
© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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