The one hour human proteome.

We describe deep analysis of the human proteome in less than one hour. We achieve this expedited proteome characterization by leveraging state-of-the-art sample preparation, chromatographic separations, data analysis tools, and by using the new Orbitrap Astral mass spectrometer equipped with a quadrupole mass filter, a high-field Orbitrap mass analyzer, and an asymmetric track lossless (Astral) mass analyzer. The system offers high MS/MS acquisition speed of 200 Hz and detects hundreds of peptide sequences per second within data independent- or data-dependent acquisition modes of operation. The fast-switching capabilities of the new quadrupole complement the sensitivity and fast ion scanning of the Astral analyzer to enable narrow-bin data-independent analysis (DIA) methods. Over a 30-minute active chromatographic method consuming a total analysis time of 56 minutes, the Q-Orbitrap-Astral hybrid MS collects an average of 4,319 MS

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Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Competing financial interest Notes The authors declare the following competing financial interest(s): The Coon lab at the University of Wisconsin-Madison has a sponsored research agreement with Thermo Fisher Scientific, the manufacturer of the instrumentation used in this research. However, analytical techniques and data analysis were performed independent of Thermo Fisher Scientific. J.J.C. is on the scientific advisory board for Thermo Fisher Scientific, Seer, and 908 Devices. T.N.A., N.E.D., A.P., M.Z., D.H., H.S., C.H., A.M., and V.Z. are employees of Thermo Fisher Scientific.