Modified polymeric biomaterials with antimicrobial and immunomodulating properties.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 Apr 2024
05 Apr 2024
Historique:
received:
01
12
2023
accepted:
02
04
2024
medline:
6
4
2024
pubmed:
6
4
2024
entrez:
5
4
2024
Statut:
epublish
Résumé
The modification of the surgical polypropylene mesh and the polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) and puromycin (aminonucleoside) yielded very stable preparations of modified biomaterials. The main emphasis was placed on analyses of their antimicrobial activity and potential immunomodulatory and non-cytotoxic properties towards the CCD841 CoTr model cell line. Cecropin A did not significantly affect the viability or proliferation of the CCD 841 CoTr cells, regardless of its soluble or immobilized form. In contrast, puromycin did not induce a significant decrease in the cell viability or proliferation in the immobilized form but significantly decreased cell viability and proliferation when administered in the soluble form. The covalent immobilization of these two molecules on the surface of biomaterials resulted in stable preparations that were able to inhibit the multiplication of Staphylococcus aureus and S. epidermidis strains. It was also found that the preparations induced the production of cytokines involved in antibacterial protection mechanisms and stimulated the immune response. The key regulator of this activity may be related to TLR4, a receptor recognizing bacterial LPS. In the present study, these factors were produced not only in the conditions of LPS stimulation but also in the absence of LPS, which indicates that cecropin A- and puromycin-modified biomaterials may upregulate pathways leading to humoral antibacterial immune response.
Identifiants
pubmed: 38580807
doi: 10.1038/s41598-024-58730-3
pii: 10.1038/s41598-024-58730-3
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8025Subventions
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Organisme : National Science Centre in Poland
ID : Opus 2017/25/B/NZ7/01084
Informations de copyright
© 2024. The Author(s).
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