Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma.
Asthma
gut microbiota
microbiome
stool
Journal
The journal of allergy and clinical immunology. Global
ISSN: 2772-8293
Titre abrégé: J Allergy Clin Immunol Glob
Pays: United States
ID NLM: 9918453488706676
Informations de publication
Date de publication:
May 2024
May 2024
Historique:
received:
05
04
2023
revised:
04
10
2023
accepted:
24
12
2023
medline:
8
4
2024
pubmed:
8
4
2024
entrez:
8
4
2024
Statut:
epublish
Résumé
Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation. This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals. Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel. Stool microbiota differed significantly between asthmatic and nonasthmatic participants ( The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher
Sections du résumé
Background
UNASSIGNED
Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation.
Objective
UNASSIGNED
This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals.
Methods
UNASSIGNED
Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel.
Results
UNASSIGNED
Stool microbiota differed significantly between asthmatic and nonasthmatic participants (
Conclusion
UNASSIGNED
The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher
Identifiants
pubmed: 38585449
doi: 10.1016/j.jacig.2024.100242
pii: S2772-8293(24)00038-9
pmc: PMC10998106
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100242Informations de copyright
© 2024 Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology.
Déclaration de conflit d'intérêts
Supported by the 10.13039/501100000781European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013), ERC grants 668954 and 101020088; the 10.13039/501100002322Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil, finance code 001; the 10.13039/501100006181Fundação de Amparo à Pesquisa do Estado da Bahia (PNE0003/2014, PNX0001/2014, and BOL0281/2020); and the 10.13039/501100003593Conselho Nacional de Desenvolvimento Científico e Tecnológico (40314 and 308521/2019-6). Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.