Development of a capillary zone electrophoresis method to monitor magnesium ion consumption during in vitro transcription for mRNA production.
Bound magnesium
Capillary zone electrophoresis
Free magnesium ion
In vitro transcription
mRNA
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
10 Apr 2024
10 Apr 2024
Historique:
received:
25
10
2023
accepted:
05
03
2024
revised:
01
03
2024
medline:
10
4
2024
pubmed:
10
4
2024
entrez:
9
4
2024
Statut:
aheadofprint
Résumé
Messenger RNA (mRNA) vaccines represent a landmark in vaccinology, especially with their success in COVID-19 vaccines, which have shown great promise for future vaccine development and disease prevention. As a platform technology, synthetic mRNA can be produced with high fidelity using in vitro transcription (IVT). Magnesium plays a vital role in the IVT process, facilitating the phosphodiester bond formation between adjacent nucleotides and ensuring accurate transcription to produce high-quality mRNA. The development of the IVT process has prompted key inquiries about in-process characterization of magnesium ion (Mg
Identifiants
pubmed: 38594392
doi: 10.1007/s00216-024-05242-8
pii: 10.1007/s00216-024-05242-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. Merck & Co., Inc., Rahway, NJ, USA and its affiliates under exclusive licence to Co-owner Consortium consisting of GDCh, SFC, SEQA, RSEQ, DAS/SCS, ASAC, SCI, PTC and Springer-Verlag GmbH, DE.
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