Phase Separation of Phytochrome B in HEK293T Cells.

Biomolecular condensates Light signal Phase separation Photobody Phytochrome B Temperature sensation

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN: 1940-6029

Titre abrégé: Methods Mol Biol

Pays: United States

ID NLM: 9214969

Informations de publication

Date de publication:
2024

Historique:

medline: 10 4 2024

pubmed: 10 4 2024

entrez: 9 4 2024

Statut: ppublish

Résumé

In this method, we employed HEK293T cells to express the plant photoreceptor phytochrome B (phyB). Through the application of various treatments such as phycocyanobilin (PCB) supplementation, red light exposure, and temperature adjustments, the phyB proteins exhibited liquid-liquid phase separation, leading to the formation of biomolecular condensates. Here, we present a comprehensive description of the protein expression, cell treatment, and imaging capture procedures. This detailed guide provides step-by-step instructions on how to induce phase separation of phyB proteins in HEK293T cells. By utilizing this approach, researchers can investigate the physicochemical characteristics and dynamic formation process of phyB photobodies with precision.

Identifiants

DOI: 10.1007/978-1-0716-3814-9_11 PMID: 38594532

pubmed: 38594532

doi: 10.1007/978-1-0716-3814-9_11

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

105-111

Informations de copyright

Références

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