An ultraviolet-driven rescue pathway for oxidative stress to eye lens protein human gamma-D crystallin.


Journal

Communications chemistry
ISSN: 2399-3669
Titre abrégé: Commun Chem
Pays: England
ID NLM: 101725670

Informations de publication

Date de publication:
10 Apr 2024
Historique:
received: 04 10 2023
accepted: 27 03 2024
medline: 11 4 2024
pubmed: 11 4 2024
entrez: 10 4 2024
Statut: epublish

Résumé

Human gamma-D crystallin (HGD) is a major constituent of the eye lens. Aggregation of HGD contributes to cataract formation, the leading cause of blindness worldwide. It is unique in its longevity, maintaining its folded and soluble state for 50-60 years. One outstanding question is the structural basis of this longevity despite oxidative aging and environmental stressors including ultraviolet radiation (UV). Here we present crystallographic structures evidencing a UV-induced crystallin redox switch mechanism. The room-temperature serial synchrotron crystallographic (SSX) structure of freshly prepared crystallin mutant (R36S) shows no post-translational modifications. After aging for nine months in the absence of light, a thiol-adduct (dithiothreitol) modifying surface cysteines is observed by low-dose SSX. This is shown to be UV-labile in an acutely light-exposed structure. This suggests a mechanism by which a major source of crystallin damage, UV, may also act as a rescuing factor in a finely balanced redox system.

Identifiants

pubmed: 38600176
doi: 10.1038/s42004-024-01163-w
pii: 10.1038/s42004-024-01163-w
doi:

Types de publication

Journal Article

Langues

eng

Pagination

81

Subventions

Organisme : Academy of Medical Sciences
ID : SBF006044
Pays : United Kingdom

Informations de copyright

© 2024. The Author(s).

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Auteurs

Jake A Hill (JA)

School of Chemistry and Biosciences, University of Bradford, Richmond Road, Bradford, BD7 1DP, United Kingdom.
School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, United Kingdom.

Yvonne Nyathi (Y)

Faculty of Biological Sciences, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, United Kingdom.

Sam Horrell (S)

Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, OX11 0DE, United Kingdom.

David von Stetten (D)

European Molecular Biology Laboratory, Notkestraße 85, 22607, Hamburg, Germany.

Danny Axford (D)

Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, OX11 0DE, United Kingdom.

Robin L Owen (RL)

Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, OX11 0DE, United Kingdom.

Godfrey S Beddard (GS)

School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, United Kingdom.
School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, United Kingdom.

Arwen R Pearson (AR)

HARBOR, Institute for Nanostructure and Solid State Physics, Hamburg, 22761, Germany.

Helen M Ginn (HM)

HARBOR, Institute for Nanostructure and Solid State Physics, Hamburg, 22761, Germany. crystallin@hginn.co.uk.
Center for Free-Electron Laser Science, CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany. crystallin@hginn.co.uk.

Briony A Yorke (BA)

School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, United Kingdom. b.a.yorke@leeds.ac.uk.

Classifications MeSH