CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis.
MSC
Osteogenesis
Single-cell RNA sequencing
Synovial chondromatosis
Synovium
Journal
Cell and tissue research
ISSN: 1432-0878
Titre abrégé: Cell Tissue Res
Pays: Germany
ID NLM: 0417625
Informations de publication
Date de publication:
11 Apr 2024
11 Apr 2024
Historique:
received:
20
02
2023
accepted:
19
03
2024
medline:
11
4
2024
pubmed:
11
4
2024
entrez:
11
4
2024
Statut:
aheadofprint
Résumé
Synovial chondromatosis (SC) is a disorder of the synovium characterized by the formation of osteochondral nodules within the synovium. This study aimed to identify the abnormally differentiated progenitor cells and possible pathogenic signaling pathways. Loose bodies and synovium were obtained from patients with SC during knee arthroplasty. Single-cell RNA sequencing was used to identify cell subsets and their gene signatures in SC synovium. Cells derived from osteoarthritis (OA) synovium were used as controls. Multi-differentiation and colony-forming assays were used to identify progenitor cells. The roles of transcription factors and signaling pathways were investigated through computational analysis and experimental verification. We identified an increased proportion of CD34+ sublining fibroblasts in SC synovium. CD34+CD31- cells and CD34-CD31- cells were sorted from SC synovium. Compared with CD34- cells, CD34+ cells had larger alkaline phosphatase (ALP)-stained area and calcified area after osteogenic induction. In addition, CD34+ cells exhibited a stronger tube formation ability than CD34- cells. Our bioinformatic analysis suggested the expression of TWIST1, a negative regulator of osteogenesis, in CD34- sublining fibroblasts and was regulated by the TGF-β signaling pathway. The experiment showed that CD34+ cells acquired the TWIST1 expression during culture and the combination of TGF-β1 and harmine, an inhibitor of Twist1, could further stimulate the osteogenesis of CD34+ cells. Overall, CD34+ synovial fibroblasts in SC synovium have multiple differentiation potentials, especially osteogenic differentiation potential, and might be responsible for the pathogenesis of SC.
Identifiants
pubmed: 38602543
doi: 10.1007/s00441-024-03892-9
pii: 10.1007/s00441-024-03892-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : The National Key R&D Program of China
ID : 2021YFA1102600
Organisme : National Natural Science Foundation of China
ID : 82002293
Organisme : National Natural Science Foundation of China
ID : 82272443
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2021A1515010294
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2021A1515010693
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2022A1515010256
Organisme : Science and Technology Planning Project of Guangzhou City, China
ID : 202201020481
Organisme : Science and Technology Planning Project of Guangzhou City, China
ID : 202201020495
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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