Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation.

Journal

Scientific reports

ISSN: 2045-2322

Titre abrégé: Sci Rep

Pays: England

ID NLM: 101563288

Informations de publication

Date de publication:
15 Apr 2024

Historique:

received: 14 11 2023

accepted: 22 03 2024

medline: 16 4 2024

pubmed: 16 4 2024

entrez: 15 4 2024

Statut: epublish

Résumé

This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage. The findings reveal that exposure to low CPA concentrations (0.5 M) did not induce significant damage to eggs. However, high concentrations (3 M) proved highly detrimental. Every cryopreservation approach investigated in this study resulted in irreversible damage to the sea urchin eggs, rendering them nonviable for future use. The research sheds light on the importance of understanding the structural alterations induced by CPAs and cryopreservation methods. This knowledge is essential for refining cryopreservation methods, potentially paving the way for successful preservation of these challenging cells.

Identifiants

DOI: 10.1038/s41598-024-57905-2 PMID: 38622199

pubmed: 38622199

doi: 10.1038/s41598-024-57905-2

pii: 10.1038/s41598-024-57905-2

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

8691

Subventions

Organisme : H2020 European Research Council

ID : 730984

Organisme : Fundacion biodiversidad, FEMP funds

ID : OCIMER+

Informations de copyright

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