Genetic variations in CYP2A6, CYP2E1, GSTM1, GSTT1 genes and the risk of Nasopharyngeal carcinoma in North African population.
CYP2E1
GSTM1
GSTT1
Nasopharyngeal Carcinoma
North Africa
metabolizing enzymes
Journal
Annals of human genetics
ISSN: 1469-1809
Titre abrégé: Ann Hum Genet
Pays: England
ID NLM: 0416661
Informations de publication
Date de publication:
15 Apr 2024
15 Apr 2024
Historique:
revised:
08
03
2024
received:
02
08
2023
accepted:
02
04
2024
medline:
16
4
2024
pubmed:
16
4
2024
entrez:
16
4
2024
Statut:
aheadofprint
Résumé
Nasopharyngeal carcinoma (NPC) is a multifactorial malignancy associated with both genetic and environmental factors. Polymorphic deletions of the phase I and phase II genes involved in the detoxification of potential carcinogens may be a risk factor for nasopharyngeal carcinoma. In this study, we investigated the relationship between CYP2E1 (rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) gene variations and NPC risk in North African countries with the highest incidence of NPC (Morocco, Algeria and Tunisia). and the evaluation of the potential use of these variants as potential biomarkers for NPC management. A total of 600 NPC cases and 545 controls frequency-matched on ethnicity, sex, age and childhood household type, were recruited from three North African countries (Morocco, Algeria and Tunisia) and analysed. Genotyping of CYP2A6 and CYP2E1(rs3813867) was performed by polymerase chain reaction restriction (PCR)-fragment length polymorphism (RFLP) analysis and the GSTM1 (rs1183423000) and GSTT1(rs1601993659) genetic variations were evaluated using the PCR technique. The genotype distributions of CYP2E1(rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) genotypes did not differ significantly among NPC cases and controls (p > 0.05). Furthermore, our data did not reveal any association with smoking and the studied variants, even when the samples were stratified by the duration period of smoking. In this large studied North African population, our findings suggest that the functional CYP2E1, CYP2A6, GSTM1 and GSTT1 variations did not influence NPC susceptibility.
Sections du résumé
BACKGROUND
BACKGROUND
Nasopharyngeal carcinoma (NPC) is a multifactorial malignancy associated with both genetic and environmental factors. Polymorphic deletions of the phase I and phase II genes involved in the detoxification of potential carcinogens may be a risk factor for nasopharyngeal carcinoma. In this study, we investigated the relationship between CYP2E1 (rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) gene variations and NPC risk in North African countries with the highest incidence of NPC (Morocco, Algeria and Tunisia). and the evaluation of the potential use of these variants as potential biomarkers for NPC management.
METHODS
METHODS
A total of 600 NPC cases and 545 controls frequency-matched on ethnicity, sex, age and childhood household type, were recruited from three North African countries (Morocco, Algeria and Tunisia) and analysed. Genotyping of CYP2A6 and CYP2E1(rs3813867) was performed by polymerase chain reaction restriction (PCR)-fragment length polymorphism (RFLP) analysis and the GSTM1 (rs1183423000) and GSTT1(rs1601993659) genetic variations were evaluated using the PCR technique.
RESULTS
RESULTS
The genotype distributions of CYP2E1(rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) genotypes did not differ significantly among NPC cases and controls (p > 0.05). Furthermore, our data did not reveal any association with smoking and the studied variants, even when the samples were stratified by the duration period of smoking.
CONCLUSION
CONCLUSIONS
In this large studied North African population, our findings suggest that the functional CYP2E1, CYP2A6, GSTM1 and GSTT1 variations did not influence NPC susceptibility.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Institut de Recherche sur le Cancer
ID : 201932
Organisme : Institut Pasteur
Organisme : International Agency for Research on Cancer
Organisme : National Scientific and Technical Research Centre
Informations de copyright
© 2024 University College London (UCL) and John Wiley & Sons Ltd.
Références
Afsar, N. A., Bruckmueller, H., Werk, A. N., Nisar, M. K., Ahmad, H. R., & Cascorbi, I. (2019). Implications of genetic variation of common drug metabolizing enzymes and ABC transporters among the Pakistani population. Scientific Reports, 9(1), 7323. https://doi.org/10.1038/s41598‐019‐43736‐z
Ahmed, S., Zhou, Z., Zhou, J., & Chen, S.‐Q. (2016). Pharmacogenomics of drug metabolizing enzymes and transporters: Relevance to precision medicine. Genomics, Proteomics & Bioinformatics, 14(5), 298–313. https://doi.org/10.1016/j.gpb.2016.03.008
Bendjemana, K., Abdennebi, M., Gara, S., Jmal, A., Ghanem, A., Touati, S., Boussen, H., Ladgham, A., & Guemira, F. (2006). Genetic polymorphism of gluthation‐S transferases and N‐acetyl transferases 2 and nasopharyngeal carcinoma: The Tunisia experience. Bulletin Du Cancer, 93(3), 297–302.
Chen, C.‐L., Liu, Q., & Relling, M. V. (1996). Simultaneous characterization of glutathione S‐transferase M1 and T1 polymorphisms by polymerase chain reaction in American whites and blacks: Pharmacogenetics, 6(2), 187–191. https://doi.org/10.1097/00008571‐199604000‐00005
Cheng, Y.‐J., Chien, Y.‐C., Hildesheim, A., Hsu, M.‐M., Chen, I.‐H., Chuang, J., Chang, J., Ma, Y. D., Luo, C.‐T., Hsu, W.‐L., Hsu, H. H.‐H., Huang, H., Chang, J.‐F., Chen, C.‐J., & Yang, C.‐S. (2003). No association between genetic polymorphisms of CYP1A1, GSTM1, GSTT1, GSTP1, NAT2, and nasopharyngeal carcinoma in Taiwan. Cancer Epidemiology, Biomarkers & Prevention, 12(2), 179–180.
Chong, E. T. J., Lee, C. C., Chua, K. H., Chuah, J. A., & Lee, P.‐C. (2014). Rsa I but not Dra I polymorphism in CYP2E1 gene increases the risk of gastrointestinal cancer in Malaysians : A case–control study. BMJ Open, 4(1), e004109. https://doi.org/10.1136/bmjopen‐2013‐004109
dos Santos, S. P., Morissugui, S. S., Gimenez Martins, A. P. D., Fernandes, G. M. de M., Russo, A., Galbiatti‐Dias, A. L. S., Castanhole‐Nunes, M. M. U., Francisco, J. L. E., Pavarino, É. C., & Goloni‐Bertollo, E. M. (2021). Evaluation of molecular markers GSTM1 and GSTT1 and clinical factors in breast cancer: Case‐control study and literature review. Xenobiotica, 51(11), 1326–1334. https://doi.org/10.1080/00498254.2021.1938291
Ezzeldin, N., El‐Lebedy, D., Darwish, A., El Bastawisy, A., Abd Elaziz, S. H., Hassan, M. M., & Saad‐Hussein, A. (2018). Association of genetic polymorphisms CYP2A6*2 rs1801272 and CYP2A6*9 rs28399433 with tobacco‐induced lung Cancer: Case‐control study in an Egyptian population. BMC Cancer, 18(1), 525. https://doi.org/10.1186/s12885‐018‐4342‐5
Farhat, F., Daulay, E. R., & Chrestella, J. (2019). The role of CYP2E1 polymorphism in the activation of procarcinogen metabolism of nasopharyngeal carcinoma. International Journal of Nasopharyngeal Carcinoma (IJNPC), 1(03), 107–109. https://doi.org/10.32734/ijnpc.v1i03.2063
Farhat, F., Daulay, E. R., Chrestella, J., & Syari, R. P. (2020). The association of CYP2E1 polymorphism and environmental factor in nasopharyngeal carcinoma patients. Open Access Macedonian Journal of Medical Sciences, 8(B), 362–367. https://doi.org/10.3889/oamjms.2020.4639
Feng, B.‐J., Jalbout, M., Ayoub, W. B., Khyatti, M., Dahmoul, S., Ayad, M., Maachi, F., Bedadra, W., Abdoun, M., Mesli, S., Hamdi‐Cherif, M., Boualga, K., Bouaouina, N., Chouchane, L., Benider, A., Ben Ayed, F., Goldgar, D., & Corbex, M. (2007). Dietary risk factors for nasopharyngeal carcinoma in Maghrebian countries. International Journal of Cancer, 121(7), 1550–1555. https://doi.org/10.1002/ijc.22813
Feng, B.‐J., Khyatti, M., Ben‐Ayoub, W., Dahmoul, S., Ayad, M., Maachi, F., Bedadra, W., Abdoun, M., Mesli, S., Bakkali, H., Jalbout, M., Hamdi‐Cherif, M., Boualga, K., Bouaouina, N., Chouchane, L., Benider, A., Ben‐Ayed, F., Goldgar, D. E., & Corbex, M. (2009). Cannabis, tobacco and domestic fumes intake are associated with nasopharyngeal carcinoma in North Africa. British Journal of Cancer, 101(7), 1207–1212. https://doi.org/10.1038/sj.bjc.6605281
Ford, J. G. (2000). Glutathione S‐transferase M1 polymorphism and lung cancer risk in African‐Americans. Carcinogenesis, 21(11), 1971–1975. https://doi.org/10.1093/carcin/21.11.1971
Fujieda, M. (2004). Evaluation of CYP2A6 genetic polymorphisms as determinants of smoking behavior and tobacco‐related lung cancer risk in male Japanese smokers. Carcinogenesis, 25(12), 2451–2458. https://doi.org/10.1093/carcin/bgh258
Fujita, K. (2006). Cytochrome P450 and anticancer drugs. Current Drug Metabolism, 7(1), 23–37. https://doi.org/10.2174/138920006774832587
Gao, C.‐M., Takezaki, T., Wu, J.‐Z., Chen, M.‐B., Liu, Y.‐T., Ding, J.‐H., Sugimura, H., Cao, J., Hamajima, N., & Tajima, K. (2007). CYP2E1 Rsa I polymorphism impacts on risk of colorectal cancer association with smoking and alcohol drinking. World Journal of Gastroenterology : WJG, 13(43), 5725–5730. https://doi.org/10.3748/wjg.v13.i43.5725
Ghanayem, B. I., McDaniel, L. P., Churchwell, M. I., Twaddle, N. C., Snyder, R., Fennell, T. R., & Doerge, D. R. (2005). Role of CYP2E1 in the epoxidation of acrylamide to glycidamide and formation of DNA and hemoglobin adducts. Toxicological Sciences, 88(2), 311–318. https://doi.org/10.1093/toxsci/kfi307
Ghania, D., Katia, B., Yahia, K., Monia, A., Hayat, D., Fethi, G., & Dalila, S. (2018). Association between genetic polymorphisms of human cytochrome CYP2E1 and risk of nasopharyngeal carcinoma in Algeria population. International Journal of Pharmacy and Pharmaceutical Sciences, 10(4), 76. https://doi.org/10.22159/ijpps.2018v10i4.23389
Guengerich, F. P. (2020). Cytochrome P450 2E1 and its roles in disease. Chemico‐biological interactions, 322, 109056. https://doi.org/10.1016/j.cbi.2020.109056
Ko, J. M.‐Y., Tsang, K. H.‐K., Dai, W., Choi, S. S. A., Leong, M. M.‐L., Ngan, R. K.‐C., Kwong, D. L.‐W., Cheng, A., Lee, A. W.‐M., Ng, W. T., Tung, S., Lee, V. H.‐F., Lam, K.‐O., Chan, C. K.‐C., & Lung, M. L. (2018). Leukocyte telomere length associates with nasopharyngeal carcinoma risk and survival in Hong Kong Chinese: Role of LTL for NPC risk and survival of Stage IV patients. International Journal of Cancer, 143(9), 2289–2298. https://doi.org/10.1002/ijc.31617
Li, S., Xue, F., Zheng, Y., Yang, P., Lin, S., Deng, Y., Xu, P., Zhou, L., Hao, Q., Zhai, Z., Wu, Y., Dai, Z., & Chen, S. (2019). GSTM1 and GSTT1 null genotype increase the risk of hepatocellular carcinoma: Evidence based on 46 studies. Cancer Cell International, 19(1), 76. https://doi.org/10.1186/s12935‐019‐0792‐3
Nosheen, M., Ishrat, M., Malik, F., Baig, R., & Kayani, M. (2010). Association of GSTM1 and GSTT1 gene deletions with risk of head and neck cancer in Pakistan: A case control study. Asian Pacific Journal of Cancer Prevention, 11(4), 881–885.
Pan, C., Zhu, G., Yan, Z., Zhou, Y., & Liu, Z. (2014). Glutathione S‐transferase T1 and M1 polymorphisms are associated with lung cancer risk in a gender‐specific manner. Oncology Research and Treatment, 37(4), 164–169. https://doi.org/10.1159/000361083
Ramzy, M. M., Solliman, M. E.‐D. M., Abdel‐Hafiz, H. A., & Salah, R. (2011). Genetic polymorphism of GSTM1 and GSTP1 in lung cancer in Egypt. Public Health, 3(1), 12.
Renaud, S., Lefebvre, A., Mordon, S., Moralès, O., & Delhem, N. (2020). Novel Therapies Boosting T Cell Immunity in Epstein Barr Virus‐Associated Nasopharyngeal Carcinoma. International Journal of Molecular Sciences, 21(12), 4292. https://doi.org/10.3390/ijms21124292
Sharma, A., Gupta, S., Sodhani, P., Singh, V., Sehgal, A., Sardana, S., Mehrotra, R., & Sharma, J. K. (2015). Glutathione S‐transferase M1 and T1 polymorphisms, cigarette smoking and HPV infection in precancerous and cancerous lesions of the uterine cervix. Asian Pacific Journal of Cancer Prevention, 16(15), 6429–6438. https://doi.org/10.7314/APJCP.2015.16.15.6429
Soeroso, N., Zain‐Hamid, R., Bihar, S., Tarigan, S. P., & Ananda, F. R. (2021). Genetic polymorphism of Cyp2a6 and Cyp2a13 genes and environmental tobacco smoke induced lung cancer risk in indonesian female never smokers. Open Access Macedonian Journal of Medical Sciences, 9(A), 1219–1225. https://doi.org/10.3889/oamjms.2021.6069
Sweeney, C., Nazar‐Stewart, V., Stapleton, P. L., Eaton, D. L., & Vaughan, T. L. (2003). Glutathione S‐transferase M1, T1, and P1 polymorphisms and survival among lung cancer patients. Cancer Epidemiology, Biomarkers & Prevention, 12(6), 527–533.
Tiwawech, D., Srivatanakul, P., Karalak, A., & Ishida, T. (2005). Glutathione S‐transferase M1 gene polymorphism in Thai nasopharyngeal carcinoma. Asian Pacific Journal of Cancer Prevention, 6(3), 270–275.
Topcu, Z. (2002). CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers in Sri Lanka. Carcinogenesis, 23(4), 595–598. https://doi.org/10.1093/carcin/23.4.595
Wei, W. I., & Sham, J. S. (2005). Nasopharyngeal carcinoma. The Lancet, 365(9476), 2041–2054. https://doi.org/10.1016/S0140‐6736(05)66698‐6
Yong, S. K., Ha, T. C., Yeo, M. C. R., Gaborieau, V., McKay, J. D., & Wee, J. (2017). Associations of lifestyle and diet with the risk of nasopharyngeal carcinoma in Singapore: A case–control study. Chinese Journal of Cancer, 36, 3. https://doi.org/10.1186/s40880‐016‐0174‐3
Young, L. S., & Dawson, C. W. (2014). Epstein‐Barr virus and nasopharyngeal carcinoma. Chinese Journal of Cancer, https://doi.org/10.5732/cjc.014.10197
Young, R., Hopkins, R. J., Hay, B. A., & Gamble, G. D. (2011). GSTM1 null genotype in COPD and lung cancer: Evidence of a modifier or confounding effect? The Application of Clinical Genetics, 4, 137–144. https://doi.org/10.2147/TACG.S21517
Zhao, F., Su, J.‐F., Lun, S.‐M., Hou, Y.‐J., Duan, L.‐J., Wang, N.‐C., Shen, F.‐F., Zhang, Y.‐W., Gao, Z.‐W., Li, J., Du, X.‐J., & Zhou, F.‐Y. (2019). Association between polymorphisms in the CYP1A1, CYP2E1 and GSTM1 genes, and smoking, alcohol and upper digestive tract carcinomas in a high‐incidence area of northern China. Oncology Letters, 18(2), 1267–1277. https://doi.org/10.3892/ol.2019.10455