Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities.
biochemical pathways
carbon partitioning
central carbon metabolism
isotopic labeling
metabolic flux analysis
metabolic modeling
plant metabolism
systems biology
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
16 Apr 2024
16 Apr 2024
Historique:
received:
04
02
2024
accepted:
06
03
2024
medline:
17
4
2024
pubmed:
17
4
2024
entrez:
17
4
2024
Statut:
aheadofprint
Résumé
Metabolic flux analysis (MFA) is a valuable tool for quantifying cellular phenotypes and to guide plant metabolic engineering. By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical pathways. Recent applications of isotopically nonstationary MFA (INST-MFA) to plants have elucidated nonintuitive metabolism in leaves under optimal and stress conditions, described coupled fluxes for fast-growing algae, and produced a synergistic multi-organ flux map that is a first in MFA for any biological system. These insights could not be elucidated through other approaches and show the potential of INST-MFA to correct an oversimplified understanding of plant metabolism.
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Science Foundation
ID : IOS-1829365
Organisme : Department of Energy, Biological and Environmental Research
ID : DE-SC0022207
Organisme : Department of Energy, Biological and Environmental Research
ID : DE-SC0023142
Organisme : National Institute of Food and Agriculture
ID : 2021-67013-33778
Informations de copyright
© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
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