RUNX transcription factors are essential in maintaining epididymal epithelial differentiation.
Development
Epididymis
Epithelial to mesenchymal transition
Loss of epithelial phenotype
MAPK signaling
NOTCH
RUNX1
RUNX2
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
17 Apr 2024
17 Apr 2024
Historique:
received:
04
09
2023
accepted:
18
03
2024
revised:
06
01
2024
medline:
18
4
2024
pubmed:
17
4
2024
entrez:
17
4
2024
Statut:
epublish
Résumé
Apart from the androgen receptor, transcription factors (TFs) that are required for the development and formation of the different segments of the epididymis have remained unknown. We identified TF families expressed in the developing epididymides, of which many showed segment specificity. From these TFs, down-regulation of runt related transcription factors (RUNXs) 1 and 2 expression coincides with epithelial regression in Dicer1 cKO mice. Concomitant deletion of both Runx1 and Runx2 in a mouse epididymal epithelial cell line affected cell morphology, adhesion and mobility in vitro. Furthermore, lack of functional RUNXs severely disturbed the formation of 3D epididymal organoid-like structures. Transcriptomic analysis of the epididymal cell organoid-like structures indicated that RUNX1 and RUNX2 are involved in the regulation of MAPK signaling, NOTCH pathway activity, and EMT-related gene expression. This suggests that RUNXs are master regulators of several essential signaling pathways, and necessary for the maintenance of proper differentiation of the epididymal epithelium.
Identifiants
pubmed: 38630262
doi: 10.1007/s00018-024-05211-5
pii: 10.1007/s00018-024-05211-5
pmc: PMC11023966
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
Core Binding Factor Alpha 2 Subunit
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
183Subventions
Organisme : Jalmari ja Rauha Ahokkaan Säätiö
ID : 0000-0001-8187-7143
Informations de copyright
© 2024. The Author(s).
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