Combined ion beam irradiation platform and 3D fluorescence microscope for cellular cancer research.

Journal

Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630

Informations de publication

Date de publication:
01 Apr 2024
Historique:
received: 06 03 2024
accepted: 06 03 2024
medline: 18 4 2024
pubmed: 18 4 2024
entrez: 18 4 2024
Statut: epublish

Résumé

To improve particle radiotherapy, we need a better understanding of the biology of radiation effects, particularly in heavy ion radiation therapy, where global responses are observed despite energy deposition in only a subset of cells. Here, we integrated a high-speed swept confocally-aligned planar excitation (SCAPE) microscope into a focused ion beam irradiation platform to allow real-time 3D structural and functional imaging of living biological samples during and after irradiation. We demonstrate dynamic imaging of the acute effects of irradiation on 3D cultures of U87 human glioblastoma cells, revealing characteristic changes in cellular movement and intracellular calcium signaling following ionizing irradiation.

Identifiants

DOI: 10.1364/BOE.522969 PMID: 38633084 PMC: PMC11019671
pubmed: 38633084
doi: 10.1364/BOE.522969
pii: 522969
pmc: PMC11019671
doi:

Types de publication

Journal Article

Langues

eng

Pagination

2561-2577

Informations de copyright

© 2024 Optica Publishing Group.

Déclaration de conflit d'intérêts

EMCH and CPC: Leica Microsystems and Applied Scientific Instrumentation (ASI) (P, R).

Auteurs

Andrew D Harken (AD)

Radiological Research Accelerator Facility, Columbia University Irving Medical Center, 136 S. Broadway, P.O. Box 21, Irvington, New York 10533, USA.
Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Naresh T Deoli (NT)

Radiological Research Accelerator Facility, Columbia University Irving Medical Center, 136 S. Broadway, P.O. Box 21, Irvington, New York 10533, USA.
Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Citlali Perez Campos (C)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.

Brian Ponnaiya (B)

Radiological Research Accelerator Facility, Columbia University Irving Medical Center, 136 S. Broadway, P.O. Box 21, Irvington, New York 10533, USA.
Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Guy Garty (G)

Radiological Research Accelerator Facility, Columbia University Irving Medical Center, 136 S. Broadway, P.O. Box 21, Irvington, New York 10533, USA.
Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Grace S Lee (GS)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.

Malte J Casper (MJ)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.
ORCID: 0000-0002-6879-691X

Shikhar Dhingra (S)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.

Wenze Li (W)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.
ORCID: 0000-0001-5256-7787

Gary W Johnson (GW)

Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Sally A Amundson (SA)

Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Peter W Grabham (PW)

Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.

Elizabeth M C Hillman (EMC)

Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Zuckerman Mind Brain Behavior Institute and Kavli Institute for Brain Sciences, Columbia University, New York, NY, 10027, USA.
elizabeth.hillman@columbia.edu.
ORCID: 0000-0001-5511-1451

David J Brenner (DJ)

Radiological Research Accelerator Facility, Columbia University Irving Medical Center, 136 S. Broadway, P.O. Box 21, Irvington, New York 10533, USA.
Center for Radiological Research, Columbia University Irving Medical Center, 630 W. 168th Street, New York, NY 10032, USA.
djb3@cumc.columbia.edu.

Classifications MeSH