Truncated protein isoforms generate diversity of protein localization and function in yeast.
Pus1
TIS profiling
Yap5
gene expression
genome annotation
meiosis
protein isoforms
ribosome profiling
truncation
yeast
Journal
Cell systems
ISSN: 2405-4720
Titre abrégé: Cell Syst
Pays: United States
ID NLM: 101656080
Informations de publication
Date de publication:
17 Apr 2024
17 Apr 2024
Historique:
received:
23
06
2023
revised:
21
01
2024
accepted:
20
03
2024
medline:
19
4
2024
pubmed:
19
4
2024
entrez:
18
4
2024
Statut:
ppublish
Résumé
Genome-wide measurement of ribosome occupancy on mRNAs has enabled empirical identification of translated regions, but high-confidence detection of coding regions that overlap annotated coding regions has remained challenging. Here, we report a sensitive and robust algorithm that revealed the translation of 388 N-terminally truncated proteins in budding yeast-more than 30-fold more than previously known. We extensively experimentally validated them and defined two classes. The first class lacks large portions of the annotated protein and tends to be produced from a truncated transcript. We show that two such cases, Yap5
Identifiants
pubmed: 38636458
pii: S2405-4712(24)00090-5
doi: 10.1016/j.cels.2024.03.005
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
388-408.e4Informations de copyright
Copyright © 2024 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.