Zinc oxide nanoparticles preserve the quality and fertility potential of rooster sperm during the cryopreservation process.
Lake extender, rooster
ZnONP
cryopreservation
fertility
spermatozoa
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
revised:
14
03
2024
received:
31
01
2024
accepted:
07
04
2024
medline:
22
4
2024
pubmed:
22
4
2024
entrez:
22
4
2024
Statut:
ppublish
Résumé
Sperm cryopreservation is one of the main methods for preserving rooster sperm for artificial insemination (AI) in commercial flocks. Yet, rooster sperm is extremely susceptible to reactive oxygen species (ROS) produced during the freezing process. Oxidative stress could be prevented by using nanoparticles containing antioxidants. The present study was conducted to investigate the effect of zinc oxide nanoparticles (ZnONP) in rooster semen freezing extender on quality parameters and fertility potential. For this aim, semen samples were collected and diluted in Lake extenders as follows: control: Lake without ZnONP, ZnO100: Lake with 100-μg zinc oxide (ZnO), ZnONP50: Lake with 50-μg ZnONP, ZnONP100: Lake with 100-μg ZnONP and ZnONP200: Lake with 200-μg ZnONP. After freezing and thawing, sperm motility, viability, membrane integrity, morphology, mitochondrial activity, acrosome integrity, DNA fragmentation, lipid peroxidation and ROS, as well as fertility and hatchability were assessed. According to the current results, higher rates of motility, membrane integrity, mitochondrial activity, acrosome integrity and live cells were detected in the ZnO100, ZnONP50 and ZnONP100 groups compared to other groups (p ≤ .05). Yet, the percentage of dead cells, DNA fragmentation, lipid peroxidation and ROS levels were lower in the mentioned groups (p ≤ .05). Furthermore, a higher percentage of fertility was observed in the ZnO100 and ZnONP100 groups than in the control group (p ≤ .05). In conclusion, the use of 100-μg ZnO and 50- to 100-μg ZnONP represents a valuable and safe additive material that could be used to improve the quality and fertility potential of rooster sperm under cryopreservation conditions.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14568Informations de copyright
© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.
Références
Afifi, M., Almaghrabi, O. A., & Kadasa, N. M. (2015). Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin‐induced diabetic rat testes. BioMed Research International, 2015, 1–6.
Amen, M. H. M., & Al‐Daraji, H. J. (2011a). Effect of dietary supplementation with different level of zinc on sperm egg penetration and fertility traits of broiler breeder chicken. Pakistan Journal of Nutrition, 10, 1083–1088.
Amen, M. H. M., & Al‐Daraji, H. J. (2011b). Effect of dietary zinc supplementation on some seminal plasma characteristics of broiler breeders' males. International Journal of Poultry Science, 10, 814–818.
Anzar, M., He, L., Buhr, M. M., Kroetsch, T. G., & Pauls, K. P. (2002). Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility. Biology of Reproduction, 66, 354–360.
Asadzadeh, N., Abdollahi, Z., Esmaeilkhanian, S., & Masoudi, R. (2021). Fertility and flow cytometry evaluations of ram frozen semen in plant‐based extender supplemented with Mito‐TEMPO. Animal Reproduction Science, 233, 106836.
Askarianzadeh, Z., Sharafi, M., & Karimi Torshizi, M. A. (2018). Sperm quality characteristics and fertilization capacity after cryopreservation of rooster semen in extender exposed to a magnetic field. Animal Reproduction Science, 198, 37–46.
Bagchi, D., Vuchetich, P. J., Bagchi, M., Tran, M. X., Krohn, R. L., Ray, S. D., & Stohs, S. J. (1998). Protective effects of zinc salts on TPA‐induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice. General Pharmacology: The Vascular System, 30, 43–50.
Donoghue, A. M., & Wishart, G. J. (2000). Storage of poultry semen. Animal Reproduction Science, 62, 213–232.
Esterbauer, H., & Cheeseman, K. H. (1990). Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4‐hydroxynonenal. Methods in Enzymology, 186, 407–421.
Evenson, D. P., & Wixon, R. (2005). Environmental toxicants cause sperm DNA fragmentation as detected by the sperm chromatin structure assay (SCSA®). Toxicology and Applied Pharmacology, 207, 532–537.
Fadl, A., Abdelnaby, E., El‐seadawy, I., Kotp, M., El‐Maaty, A. M. A., & El‐Sherbiny, H. (2022). Eco‐friendly synthesized zinc oxide nanoparticles improved frozen‐thawed semen quality and antioxidant capacity of rams. Journal of Advanced Veterinary Research, 12, 259–264.
Fang, L., Bai, C., Chen, Y., Dai, J., Xiang, Y., Ji, X., Huang, C., & Dong, Q. (2014). Inhibition of ROS production through mitochondria‐targeted antioxidant and mitochondrial uncoupling increases post‐thaw sperm viability in yellow catfish. Cryobiology, 69, 386–393.
Farhadi, F., Towhidi, A., Shakeri, M., & Seifi‐Jamadi, A. (2022). Zinc oxide nanoparticles have beneficial effect on frozen‐thawed spermatozoa of Holstein bulls. Iranian Journal of Applied Animal Science, 12, 49–55.
Gavella, M., & Lipovac, V. (1998). In vitro effect of zinc on oxidative changes in human semen. Andrologia, 30, 317–323.
Hatami, M., Qasemi‐panahi, B., Daghigh Kia, H., Moghaddam, G., & Janmohammadi, H. (2023). Egg yolk plasma enriched with β‐carotene through the diet of laying hens and adding it to the extender improves the quality of frozen semen in Arabic stallions. Reproduction in Domestic Animals, 58, 630–636.
Hezavehei, M., Kouchesfahani, H. M., Shahverdi, A., Sharafi, M., Salekdeh, G. H., & Eftekhari‐Yazdi, P. (2019). Induction of sublethal oxidative stress on human sperm before cryopreservation: A time‐dependent response in post‐thawed sperm parameters. Cell Journal, 20, 537–543.
Hosseinifar, H., Yazdanikhah, S., Modarresi, T., Totonchi, M., Sadighi Gilani, M. A., & Sabbaghian, M. (2015). Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients. Andrology, 3, 526–531.
Isaac, A. V., Kumari, S., Nair, R., Urs, D. R., Salian, S. R., Kalthur, G., Adiga, S. K., Manikkath, J., Mutalik, S., Sachdev, D., & Pasricha, R. (2017). Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze‐thaw‐induced damage to spermatozoa. Biochemical and Biophysical Research Communications, 494, 656–662.
Jahanshahi, M., & Myrnya, S. (2011). Nanomaterials toxicity, health and environmental concerns.
Januskauskas, A., Johannisson, A., & Rodriguez‐Martinez, H. (2003). Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology, 60, 743–758.
Jurowski, K., Szewczyk, B., Nowak, G., & Piekoszewski, W. (2014). Biological consequences of zinc deficiency in the pathomechanisms of selected diseases. JBIC Journal of Biological Inorganic Chemistry, 19, 1069–1079.
Khodaei‐Motlagh, M., Masoudi, R., Karimi‐Sabet, M. J., & Hatefi, A. (2022). Supplementation of sperm cooling medium with zinc and zinc oxide nanoparticles preserves rooster sperm quality and fertility potential. Theriogenology, 183, 36–40.
Khoobbakht, Z., Mohammadi, M., Mehr, M. R. A., Mohammadghasemi, F., & Sohani, M. M. (2018). Comparative effects of zinc oxide, zinc oxide nanoparticle and zinc‐methionine on hatchability and reproductive variables in male Japanese quail. Animal Reproduction Science, 192, 84–90.
Lake, P. E., & Ravie, O. (1984). An exploration of cryoprotective compounds for fowl spermatozoa. British Poultry Science, 25, 145–150.
Lee, J. A., Spidlen, J., Boyce, K., Cai, J., Crosbie, N., Dalphin, M., Furlong, J., Gasparetto, M., Goldberg, M., Goralczyk, E. M., Hyun, B., Jansen, K., Kollmann, T., Kong, M., Leif, R., McWeeney, S., Moloshok, T. D., Moore, W., Nolan, G., … Brinkman, R. R. (2008). MIFlowCyt: The minimum information about a flow cytometry experiment. Cytometry. Part A: the Journal of the International Society for Analytical Cytology, 73, 926–930.
Lewis‐Jones, D. I., Aird, I. A., Biljan, M. M., & Kingsland, C. R. (1996). Andrology: Effects of sperm activity on zinc and fructose concentrations in seminal plasma. Human Reproduction, 11, 2465–2467.
Long, J. A., & Kulkarni, G. (2004). An effective method for improving the fertility of glycerol‐exposed poultry semen. Poultry Science, 83, 1594–1601.
Masoudi, R., Esmaeilkhanian, S., Sharafi, M., Abdollahi, Z., Jafari, V., Hatefi, A., Zarei, F., Asadzadeh, N., Sadeghipanah, A., & Barfourooshi, H. J. (2022). Cysteamine enhances quality and fertility potential of rooster semen in cooled storage. Theriogenology, 177, 29–33.
Niki, E. (1991). Action of ascorbic acid as a scavenger of active and stable oxygen radicals. The American Journal of Clinical Nutrition, 54, 1119–1124.
Ruiz‐Pesini, E., Díez‐Sánchez, C., López‐Pérez, M. J., & Enríquez, J. A. (2007). The role of the mitochondrion in sperm function: Is there a place for oxidative phosphorylation or is this a purely glycolytic process? Current Topics in Developmental Biology, 77, 3–19.
Schaäfer, S., & Holzmann, A. (2000). The use of transmigration and Spermac™ stain to evaluate epididymal cat spermatozoa. Animal Reproduction Science, 59, 201–211.
Shahverdi, A., Sharafi, M., Gourabi, H., Yekta, A. A., Esmaeili, V., Sharbatoghli, M., Janzamin, E., Hajnasrollahi, M., & Mostafayi, F. (2015). Fertility and flow cytometric evaluations of frozen‐thawed rooster semen in cryopreservation medium containing low‐density lipoprotein. Theriogenology, 83, 78–85.
Sharma, R., Roychoudhury, S., Singh, N., & Sarda, Y. (2017). Methods to measure reactive oxygen species (ROS) and total antioxidant capacity (TAC) in the reproductive system. In Oxidative stress in human reproduction: Shedding light on a complicated phenomenon (Vol. 6, pp. 17–46). Springer International Publishing.
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60, 481–492.