Improved succinic acid production through the reconstruction of methanol dissimilation in Escherichia coli.
C1-substrates utilization
CO2 fixation
Methanol dissimilation
Succinic acid fermentation
Synthetic biology
Journal
Bioresources and bioprocessing
ISSN: 2197-4365
Titre abrégé: Bioresour Bioprocess
Pays: Germany
ID NLM: 101665551
Informations de publication
Date de publication:
31 May 2022
31 May 2022
Historique:
received:
15
03
2022
accepted:
03
05
2022
medline:
31
5
2022
pubmed:
31
5
2022
entrez:
22
4
2024
Statut:
epublish
Résumé
Synthetic biology has boosted the rapid development on using non-methylotrophy as chassis for value added chemicals production from one-carbon feedstocks, such as methanol and formic acid. The one-carbon dissimilation pathway can provide more NADH than monosaccharides including glucose, which is conducive for reductive chemicals production, such as succinic acid. In this study, the one-carbon dissimilation pathway was introduced in E. coli Suc260 to enhance the succinic acid production capability. Through the rational construction of methanol dissimilation pathway, the succinic acid yield was increased from 0.91 to 0.95 g/g with methanol and sodium formate as auxiliary substrates in anaerobic fed-batch fermentation. Furthermore, the metabolic flux of by-product pyruvate was redirected to succinic acid together with the CO
Identifiants
pubmed: 38647636
doi: 10.1186/s40643-022-00547-x
pii: 10.1186/s40643-022-00547-x
doi:
Types de publication
Journal Article
Langues
eng
Pagination
62Subventions
Organisme : National Key R&D Program of China
ID : 2018YFA0901500
Organisme : National Natural Science Foundation of China
ID : 22078151
Organisme : National Natural Science Foundation of China
ID : 22178169
Organisme : National Natural Science Foundation of China
ID : 22008113
Organisme : Natural Science Foundation of Jiangsu Province
ID : BK20200683
Informations de copyright
© 2022. The Author(s).
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