Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture.
Microchloropsis salina
Tisochrysis lutea
Co-cultivation
Docosahexaenoic acid (DHA)
Eicosapentaenoic acid (EPA)
Photoautotrophic microalgae
Journal
Bioresources and bioprocessing
ISSN: 2197-4365
Titre abrégé: Bioresour Bioprocess
Pays: Germany
ID NLM: 101665551
Informations de publication
Date de publication:
19 Dec 2022
19 Dec 2022
Historique:
received:
19
08
2022
accepted:
18
11
2022
medline:
19
12
2022
pubmed:
19
12
2022
entrez:
22
4
2024
Statut:
epublish
Résumé
Marine microalgae have received much attention as a sustainable source of the two health beneficial omega-3-fatty acids docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5). However, photoautotrophic monocultures of microalgae can only produce either DHA or EPA enriched biomass. An alternative may be the photoautotrophic co-cultivation of Tisochrysis lutea as DHA-producer with Microchloropsis salina for simultaneous EPA production to obtain EPA- and DHA-rich microalgae biomass in a nutritionally balanced ratio. Photoautotrophic co-cultivation processes of T. lutea and M. salina were studied, applying scalable and fully controlled lab-scale gas-lift flat-plate photobioreactors with LED illumination for dynamic climate simulation of a repeated sunny summer day in Australia [day-night cycles of incident light (PAR) and temperature]. Monocultures of both marine microalgae were used as reference batch processes. Differences in the autofluorescence of both microalgae enabled the individual measurement, of cell distributions in co-culture, by flow cytometry. The co-cultivation of T. lutea and M. salina in artificial sea water with an inoculation ratio of 1:3 resulted in a balanced biomass production of both microalgae simultaneously with a DHA:EPA ratio of almost 1:1 (26 mg
Identifiants
pubmed: 38647795
doi: 10.1186/s40643-022-00612-5
pii: 10.1186/s40643-022-00612-5
doi:
Types de publication
Journal Article
Langues
eng
Pagination
130Informations de copyright
© 2022. The Author(s).
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