Point mutation of V252 in neomycin C epimerase enlarges substrate-binding pocket and improves neomycin B accumulation in Streptomyces fradiae.
S. fradiae
Catalytic mechanism
NeoN
Neomycin
Journal
Bioresources and bioprocessing
ISSN: 2197-4365
Titre abrégé: Bioresour Bioprocess
Pays: Germany
ID NLM: 101665551
Informations de publication
Date de publication:
05 Dec 2022
05 Dec 2022
Historique:
received:
20
09
2022
accepted:
19
11
2022
medline:
5
12
2022
pubmed:
5
12
2022
entrez:
22
4
2024
Statut:
epublish
Résumé
Neomycin, an aminoglycoside antibiotic with broad-spectrum antibacterial resistance, is widely used in pharmaceutical and agricultural fields. However, separation and purification of neomycin B as an active substance from Streptomyces fradiae are complicated. Although NeoN can catalyze conversion of neomycin C to neomycin B, the underlying catalytic mechanism is still unclear. In this study, the genomic information of high-yielding mutant S. fradiae SF-2 was elucidated using whole-genome sequencing. Subsequently, the mechanism of NeoN in catalyzing conversion of neomycin C to neomycin B was resolved based on NeoN-SAM-neomycin C ternary complex. Mutant NeoN
Identifiants
pubmed: 38647873
doi: 10.1186/s40643-022-00613-4
pii: 10.1186/s40643-022-00613-4
doi:
Types de publication
Journal Article
Langues
eng
Pagination
123Subventions
Organisme : National Natural Science Foundation of China
ID : (31471615
Organisme : National Natural Science Foundation of China
ID : 31871781
Organisme : National Natural Science Foundation of China
ID : 31772081)
Informations de copyright
© 2022. The Author(s).
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