Protein engineering a PhotoRNR chimera based on a unifying evolutionary apparatus among the natural classes of ribonucleotide reductases.
10-stranded αβ barrel
chimeric enzyme
proton-coupled electron transfer
radical transport
ribonucleotide reductase
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
30 Apr 2024
30 Apr 2024
Historique:
medline:
23
4
2024
pubmed:
23
4
2024
entrez:
22
4
2024
Statut:
ppublish
Résumé
Ribonucleotide reductases (RNRs) are essential enzymes that catalyze the de novo transformation of nucleoside 5'-di(tri)phosphates [ND(T)Ps, where N is A, U, C, or G] to their corresponding deoxynucleotides. Despite the diversity of factors required for function and the low sequence conservation across RNRs, a unifying apparatus consolidating RNR activity is explored. We combine aspects of the protein subunit simplicity of class II RNR with a modified version of
Identifiants
pubmed: 38648489
doi: 10.1073/pnas.2317291121
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2317291121Subventions
Organisme : HHS | National Institutes of Health (NIH)
ID : GM047274
Déclaration de conflit d'intérêts
Competing interests statement:The authors declare no competing interest.