Nonlinear relationship between viral load and TCT in single/multiple HPV52 infection.
Humans
Female
Viral Load
Papillomavirus Infections
/ virology
Adult
Middle Aged
Uterine Cervical Neoplasms
/ virology
Early Detection of Cancer
/ methods
Papillomaviridae
/ genetics
Genotype
Aged
Vaginal Smears
Coinfection
/ virology
Young Adult
Uterine Cervical Dysplasia
/ virology
DNA, Viral
/ genetics
Human Papillomavirus Viruses
Alphapapillomavirus
HPV viral load
Multiple HPV infections
Single HPV infection
TCT
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
23 Apr 2024
23 Apr 2024
Historique:
received:
19
11
2023
accepted:
02
04
2024
medline:
24
4
2024
pubmed:
24
4
2024
entrez:
23
4
2024
Statut:
epublish
Résumé
To determine the correlation between HPV (human papillomavirus) 52 viral load, multiple infections and ThinPrep cytology test (TCT), to inform clinical management of HPV52-positive women after cervical cancer screening. A total of 1,882 female patients who had positive quantitative HPV tests at Yuebei People's Hospital from January 2020 to December 2022, of whom 533 tested positive for HPV52. We excluded patients who combined HPV16 and/or HPV 18 positivity and whom HPV52 viral load could not be calculated. The final enrollment was 488 patients, including 400 NILM, 48 ASC-US, 28 LSIL and 12 HSIL. The HPV test is a quantitative multiplexed fluorescent PCR assay that provides both HPV genotyping and viral load. In our study, there were differences in the median distribution of viral loads among various cytological class categories. The risk of TCT results (LSIL or worse) was increased with the increase of HPV52 viral load, for every LOG unit increase in HPV52 viral load, the risk increased by 26.6%. More importantly, we found a nonlinear relationship between HPV52 viral load and TCT results (LSIL or worse) in both single and multiple infections. When the viral load reaches a threshold, the risk of abnormal cytological results increases significantly. HPV52 viral load is an independent risk factor for TCT results (LSIL or worse). The relationship between HPV52 viral load and TCT results (LSIL or worse) is not linear. Viral load may be used as a triage indicator for HPV52-positive patients, thus improving the post-screening clinical management of HPV52-positive women.
Identifiants
pubmed: 38654353
doi: 10.1186/s12985-024-02356-4
pii: 10.1186/s12985-024-02356-4
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
90Informations de copyright
© 2024. The Author(s).
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