Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen.
Biochemistry
Biochemistry applications
Biochemistry methods
Medical biochemistry
Proteomics
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
17 May 2024
17 May 2024
Historique:
received:
17
03
2023
revised:
30
09
2023
accepted:
02
04
2024
medline:
24
4
2024
pubmed:
24
4
2024
entrez:
24
4
2024
Statut:
epublish
Résumé
This study investigates the efficacy of proteomic analysis of human remains to identify active infections in the past through the detection of pathogens and the host response to infection. We advance leprosy as a case study due to the sequestering of sufferers in leprosaria and the suggestive skeletal lesions that can result from the disease. Here we present a sequential enzyme extraction protocol, using trypsin followed by ProAlanase, to reduce the abundance of collagen peptides and in so doing increase the detection of non-collagenous proteins. Through our study of five individuals from an 11th to 18th century leprosarium, as well as four from a contemporaneous non-leprosy associated cemetery in Barcelona, we show that samples from 2 out of 5 leprosarium individuals extracted with the sequential digestion methodology contain numerous host immune proteins associated with modern leprosy. In contrast, individuals from the non-leprosy associated cemetery and all samples extracted with a trypsin-only protocol did not. Through this study, we advance a palaeoproteomic methodology to gain insights into the health of archaeological individuals and take a step toward a proteomics-based method to study immune responses in past populations.
Identifiants
pubmed: 38655200
doi: 10.1016/j.isci.2024.109663
pii: S2589-0042(24)00885-X
pmc: PMC11035369
doi:
Types de publication
Journal Article
Langues
eng
Pagination
109663Informations de copyright
© 2024 Published by Elsevier Inc.
Déclaration de conflit d'intérêts
We have no conflicting interest to declare.
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