Measurement of Feline Alpha-1 Acid Glycoprotein in Serum and Effusion Using an ELISA Method: Analytical Validation and Diagnostic Role for Feline Infectious Peritonitis.
AGP
FIP
accuracy
acute-phase proteins
likelihood ratio
sensitivity
specificity
validation
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
29 Mar 2024
29 Mar 2024
Historique:
received:
13
03
2024
revised:
22
03
2024
accepted:
24
03
2024
medline:
26
4
2024
pubmed:
26
4
2024
entrez:
26
4
2024
Statut:
epublish
Résumé
Alpha-1 acid glycoprotein (AGP) may support a clinical diagnosis of feline infectious peritonitis (FIP). In this study, we assessed the analytical and diagnostic performances of a novel ELISA method to measure feline AGP. AGP was measured in sera and effusions from cats with FIP (n = 20) or with other diseases (n = 15). Precision was calculated based on the coefficient of variation (CV) of repeated testing, and accuracy was calculated by linearity under dilution (LUD). The test is precise (intra-assay CVs: <6.0% in individual samples, <15.0% in pooled samples; inter-assay CVs <11.0% and <15.0%) and accurate (serum LUD r This ELISA method is precise and accurate. AGP in serum and in effusions is a useful diagnostic marker for FIP.
Sections du résumé
BACKGROUND
BACKGROUND
Alpha-1 acid glycoprotein (AGP) may support a clinical diagnosis of feline infectious peritonitis (FIP). In this study, we assessed the analytical and diagnostic performances of a novel ELISA method to measure feline AGP.
METHODS
METHODS
AGP was measured in sera and effusions from cats with FIP (n = 20) or with other diseases (n = 15). Precision was calculated based on the coefficient of variation (CV) of repeated testing, and accuracy was calculated by linearity under dilution (LUD).
RESULTS
RESULTS
The test is precise (intra-assay CVs: <6.0% in individual samples, <15.0% in pooled samples; inter-assay CVs <11.0% and <15.0%) and accurate (serum LUD r
CONCLUSION
CONCLUSIONS
This ELISA method is precise and accurate. AGP in serum and in effusions is a useful diagnostic marker for FIP.
Identifiants
pubmed: 38668244
pii: pathogens13040289
doi: 10.3390/pathogens13040289
pii:
doi:
Types de publication
Journal Article
Langues
eng