First Specific Detection of Mammalian Orthoreovirus from Goats Using TaqMan Real-Time RT-PCR Technology.

TaqMan qRT-PCR detection goat mammalian orthoreovirus sheep

Journal

Veterinary sciences
ISSN: 2306-7381
Titre abrégé: Vet Sci
Pays: Switzerland
ID NLM: 101680127

Informations de publication

Date de publication:
22 Mar 2024
Historique:
received: 29 01 2024
revised: 08 03 2024
accepted: 16 03 2024
medline: 26 4 2024
pubmed: 26 4 2024
entrez: 26 4 2024
Statut: epublish

Résumé

Mammalian orthoreovirus (MRV) infections are ubiquitous in multiple mammalian species including humans, and mainly causes gastroenteritis and respiratory disease. In this study, we developed a rapid and sensitive TaqMan qRT-PCR method for MRV detection based on the primers and probe designed within the conserved L1 gene. The qRT-PCR assay was evaluated for its sensitivity, specificity, efficiency and reproducibility. It was found that the detection sensitivity was equivalent to 10 DNA copies/μL, and the standard curves had a linear correlation of R

Identifiants

DOI: 10.3390/vetsci11040141 PMID: 38668409
pubmed: 38668409
pii: vetsci11040141
doi: 10.3390/vetsci11040141
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
ID : SKLVBF202106
Organisme : State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute
ID : SKLVEB2021KFKT005
Organisme : National Natural Science Foundation of China
ID : 31802196, 31960710
Organisme : Jiangsu Agriculture Science and Technology Innovation Fund
ID : CX(23)1029
Organisme : Innovation Capability Support Program of Shaanxi Province
ID : 2021PT-017

Auteurs

Li Mao (L)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China.

Xia Li (X)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.

Xuhang Cai (X)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China.

Wenliang Li (W)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China.

Jizong Li (J)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China.

Shanshan Yang (S)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China.
ORCID: 0009-0000-7748-0223

Junjun Zhai (J)

Shaanxi Province Engineering and Technology Research Center of Cashmere Goat, Yulin University, Yulin 719000, China.

Sizhu Suolang (S)

College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.

Bin Li (B)

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China.
National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225000, China.
ORCID: 0000-0003-1318-7081

Classifications MeSH