VC-resist glioblastoma cell state: vessel co-option as a key driver of chemoradiation resistance.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
29 Apr 2024
Historique:
received: 10 11 2023
accepted: 17 04 2024
medline: 30 4 2024
pubmed: 30 4 2024
entrez: 29 4 2024
Statut: epublish

Résumé

Glioblastoma (GBM) is a highly lethal type of cancer. GBM recurrence following chemoradiation is typically attributed to the regrowth of invasive and resistant cells. Therefore, there is a pressing need to gain a deeper understanding of the mechanisms underlying GBM resistance to chemoradiation and its ability to infiltrate. Using a combination of transcriptomic, proteomic, and phosphoproteomic analyses, longitudinal imaging, organotypic cultures, functional assays, animal studies, and clinical data analyses, we demonstrate that chemoradiation and brain vasculature induce cell transition to a functional state named VC-Resist (vessel co-opting and resistant cell state). This cell state is midway along the transcriptomic axis between proneural and mesenchymal GBM cells and is closer to the AC/MES1-like state. VC-Resist GBM cells are highly vessel co-opting, allowing significant infiltration into the surrounding brain tissue and homing to the perivascular niche, which in turn induces even more VC-Resist transition. The molecular and functional characteristics of this FGFR1-YAP1-dependent GBM cell state, including resistance to DNA damage, enrichment in the G2M phase, and induction of senescence/stemness pathways, contribute to its enhanced resistance to chemoradiation. These findings demonstrate how vessel co-option, perivascular niche, and GBM cell plasticity jointly drive resistance to therapy during GBM recurrence.

Identifiants

pubmed: 38684700
doi: 10.1038/s41467-024-47985-z
pii: 10.1038/s41467-024-47985-z
doi:

Substances chimiques

YAP-Signaling Proteins 0
YAP1 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

3602

Subventions

Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 805225
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 805225
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 805225
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 805225
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2020-1-PLBIO-01-ICR-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2021-1-PAIR-CEREB-01-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2020-1-PLBIO-01-ICR-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2021-1-PAIR-CEREB-01-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2020-1-PLBIO-01-ICR-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2021-1-PAIR-CEREB-01-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2020-1-PLBIO-01-ICR-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2021-1-PAIR-CEREB-01-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2020-1-PLBIO-01-ICR-1
Organisme : Institut National Du Cancer (French National Cancer Institute)
ID : INCa-2021-1-PAIR-CEREB-01-1
Organisme : Centre National de la Recherche Scientifique (National Center for Scientific Research)
ID : ATIP-Avenir
Organisme : Université Paris-Saclay (University of Paris-Saclay)
ID : NanoTheRad

Informations de copyright

© 2024. The Author(s).

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Auteurs

Cathy Pichol-Thievend (C)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Oceane Anezo (O)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Aafrin M Pettiwala (AM)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.
Institut Curie, PSL University, 75005, Paris, France.

Guillaume Bourmeau (G)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Remi Montagne (R)

Institut Curie, PSL University, 75005, Paris, France.
INSERM U900, 75005, Paris, France.
MINES ParisTeach, CBIO-Centre for Computational Biology, PSL Research University, 75006, Paris, France.

Anne-Marie Lyne (AM)

Institut Curie, PSL University, 75005, Paris, France.
INSERM U900, 75005, Paris, France.
MINES ParisTeach, CBIO-Centre for Computational Biology, PSL Research University, 75006, Paris, France.

Pierre-Olivier Guichet (PO)

Université de Poitiers, CHU Poitiers, ProDiCeT, F-86000, Poitiers, France.
CHU Poitiers, Laboratoire de Cancérologie Biologique, F-86000, Poitiers, France.

Pauline Deshors (P)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Alberto Ballestín (A)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Benjamin Blanchard (B)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Juliette Reveilles (J)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Vidhya M Ravi (VM)

Department of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.

Kevin Joseph (K)

Department of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.

Dieter H Heiland (DH)

Department of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.

Boris Julien (B)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Sophie Leboucher (S)

Histology Facility, Institut Curie, 91400, Orsay, France.

Laetitia Besse (L)

Institut Curie, PSL University, Université Paris-Saclay, CNRS UMS2016, INSERM US43, Multimodal Imaging Center, 91400, Orsay, France.

Patricia Legoix (P)

Institut Curie, PSL University, ICGex Next-Generation Sequencing Platform, 75005, Paris, France.

Florent Dingli (F)

Institut Curie, PSL University, CurieCoreTech Spectrométrie de Masse Protéomique, 75005, Paris, France.

Stephane Liva (S)

Institut Curie, PSL University, 75005, Paris, France.
INSERM U900, 75005, Paris, France.
MINES ParisTeach, CBIO-Centre for Computational Biology, PSL Research University, 75006, Paris, France.

Damarys Loew (D)

Institut Curie, PSL University, CurieCoreTech Spectrométrie de Masse Protéomique, 75005, Paris, France.

Elisa Giani (E)

Department of Biomedical Sciences, Humanitas University, 20072, Pieve Emanuele, Italy.

Valentino Ribecco (V)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Charita Furumaya (C)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Laura Marcos-Kovandzic (L)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France.

Konstantin Masliantsev (K)

Université de Poitiers, CHU Poitiers, ProDiCeT, F-86000, Poitiers, France.
CHU Poitiers, Laboratoire de Cancérologie Biologique, F-86000, Poitiers, France.

Thomas Daubon (T)

Université Bordeaux, CNRS, IBGC, UMR5095, Bordeaux, France.

Lin Wang (L)

Department of Computational and Quantitative Medicine, Hematologic Malignancies Research Institute and Beckman Research Institute, City of Hope, Duarte, CA, USA.

Aaron A Diaz (AA)

Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.

Oliver Schnell (O)

Department of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.

Jürgen Beck (J)

Department of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.

Nicolas Servant (N)

Institut Curie, PSL University, 75005, Paris, France.
INSERM U900, 75005, Paris, France.
MINES ParisTeach, CBIO-Centre for Computational Biology, PSL Research University, 75006, Paris, France.

Lucie Karayan-Tapon (L)

Université de Poitiers, CHU Poitiers, ProDiCeT, F-86000, Poitiers, France.
CHU Poitiers, Laboratoire de Cancérologie Biologique, F-86000, Poitiers, France.

Florence M G Cavalli (FMG)

Institut Curie, PSL University, 75005, Paris, France.
INSERM U900, 75005, Paris, France.
MINES ParisTeach, CBIO-Centre for Computational Biology, PSL Research University, 75006, Paris, France.

Giorgio Seano (G)

Institut Curie, INSERM U1021, CNRS UMR3347, Tumor Microenvironment Lab, Paris-Saclay University, 91400, Orsay, France. giorgio.seano@curie.fr.

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