Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas.

Animals Imaging, Three-Dimensional / methods Pancreas / diagnostic imaging Swine Multimodal Imaging / methods X-Ray Microtomography / methods Islets of Langerhans / diagnostic imaging Tomography, X-Ray Computed / methods
Computed tomography Imaging Islets of Langerhans Pancreas Synchrotron Vascularization Virtual histology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
02 May 2024
Historique:
received: 12 11 2023
accepted: 20 04 2024
medline: 3 5 2024
pubmed: 3 5 2024
entrez: 2 5 2024
Statut: epublish

Résumé

Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.

Identifiants

DOI: 10.1038/s41598-024-60254-9 PMID: 38698049
pubmed: 38698049
doi: 10.1038/s41598-024-60254-9
pii: 10.1038/s41598-024-60254-9
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

10136

Subventions

Organisme : Horizon 2020 Framework Programme
ID : E!12021
Organisme : Heidelberger Stiftung Chirurgie
ID : 2020/393

Informations de copyright

© 2024. The Author(s).

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Auteurs

Gabriel Alexander Salg (GA)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany. gabriel.salg@med.uni-heidelberg.de.
Medical Faculty, Heidelberg University, Heidelberg, Germany. gabriel.salg@med.uni-heidelberg.de.
ORCID: 0000-0002-3964-3527

Verena Steinle (V)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Division of Radiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.

Jonas Labode (J)

Institute of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Willi Wagner (W)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Translational Lung Research Center, Member of the German Center for Lung Research, University of Heidelberg, Im Neuenheimer Feld 130.3, 69120, Heidelberg, Germany.

Alexander Studier-Fischer (A)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Johanna Reiser (J)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Elyes Farjallah (E)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Michelle Guettlein (M)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Jonas Albers (J)

Hamburg Unit, European Molecular Biology Laboratory, c/o Deutsches Elektronen-Synchrotron DESY Hamburg, Notkestr. 85, 22607, Hamburg, Germany.

Tim Hilgenfeld (T)

Department of Neuroradiology, University Hospital Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Germany.

Nathalia A Giese (NA)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Wolfram Stiller (W)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Translational Lung Research Center, Member of the German Center for Lung Research, University of Heidelberg, Im Neuenheimer Feld 130.3, 69120, Heidelberg, Germany.

Felix Nickel (F)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Clinic for General-, Visceral- and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany.

Martin Loos (M)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Christoph W Michalski (CW)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Hans-Ulrich Kauczor (HU)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Translational Lung Research Center, Member of the German Center for Lung Research, University of Heidelberg, Im Neuenheimer Feld 130.3, 69120, Heidelberg, Germany.

Thilo Hackert (T)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Clinic for General-, Visceral- and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany.

Christian Dullin (C)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.
Translational Lung Research Center, Member of the German Center for Lung Research, University of Heidelberg, Im Neuenheimer Feld 130.3, 69120, Heidelberg, Germany.
Institute for Diagnostic and Interventional Radiology, University Medical Center Goettingen, Robert-Koch-Str. 40, Goettingen, Germany.
Translational Molecular Imaging, Max Planck Institute for Multidisciplinary Sciences, Hermann-Rein-Str. 3, Göttingen, Germany.

Philipp Mayer (P)

Clinic for Diagnostic and Interventional Radiology, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

Hannes Goetz Kenngott (HG)

Clinic for General-, Visceral- and Transplantation Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 420, 69120, Heidelberg, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Multiscale and multimodal imaging for...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Multiscale and multimodal imaging for...
questionsmedicales.fr › Système digestif › Pancréas › Multiscale and multimodal imaging for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Multiscale and multimodal imaging for...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Imagerie multimodale › Multiscale and multimodal imaging for...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Tomographie à rayons X › Tomodensitométrie › Microtomographie aux rayons X › Multiscale and multimodal imaging for...
questionsmedicales.fr › Système endocrine › Glandes endocrines › Ilots pancréatiques › Multiscale and multimodal imaging for...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Tomographie à rayons X › Tomodensitométrie › Multiscale and multimodal imaging for...