Metabolic priming of GD2

CAR T cells CRISPR GMP-compatible biomanufacturing cancer gene editing metabolism pre-clinical scale-up stem cell memory

Journal

Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857

Informations de publication

Date de publication:
13 Jun 2024
Historique:
received: 19 01 2024
accepted: 05 04 2024
medline: 3 5 2024
pubmed: 3 5 2024
entrez: 3 5 2024
Statut: epublish

Résumé

Manufacturing chimeric antigen receptor (CAR) T cell therapies is complex, with limited understanding of how medium composition impacts T cell phenotypes. CRISPR-Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting the endogenous T cell receptor alpha constant (

Identifiants

DOI: 10.1016/j.omtm.2024.101249 PMID: 38699288 PMC: PMC11063605
pubmed: 38699288
doi: 10.1016/j.omtm.2024.101249
pii: S2329-0501(24)00065-2
pmc: PMC11063605
doi:

Types de publication

Journal Article

Langues

eng

Pagination

101249

Informations de copyright

© 2024 The Authors.

Déclaration de conflit d'intérêts

K.S. receives honoraria for advisory board membership for Andson Biotech and Notch Therapeutics. C.M.C. receives honoraria for advisory board membership for Bayer, Elephas, Nektar Therapeutics, Novartis, and WiCell Research Institute.

Auteurs

Dan Cappabianca (D)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.

Dan Pham (D)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.
Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI 53715, USA.

Matthew H Forsberg (MH)

Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.

Madison Bugel (M)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.

Anna Tommasi (A)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.

Anthony Lauer (A)

Promega Corporation, Fitchburg, WI 53711, USA.

Jolanta Vidugiriene (J)

Promega Corporation, Fitchburg, WI 53711, USA.

Brookelyn Hrdlicka (B)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.

Alexandria McHale (A)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.

Quaovi H Sodji (QH)

University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI 53705, USA.
Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.

Melissa C Skala (MC)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.
Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI 53715, USA.
University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI 53705, USA.

Christian M Capitini (CM)

Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.
University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI 53705, USA.

Krishanu Saha (K)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53715, USA.
University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI 53705, USA.

Classifications MeSH