MtPIN4 plays critical roles in amino acid biosynthesis and metabolism of seed in Medicago truncatula.
Medicago truncatula
PIN family
amino acid content
auxin
embryo development
seed development
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
03 May 2024
03 May 2024
Historique:
revised:
20
03
2024
received:
21
06
2023
accepted:
08
04
2024
medline:
3
5
2024
pubmed:
3
5
2024
entrez:
3
5
2024
Statut:
aheadofprint
Résumé
The regulation of seed development is critical for determining crop yield. Auxins are vital phytohormones that play roles in various aspects of plant growth and development. However, its role in amino acid biosynthesis and metabolism in seeds is not fully understood. In this study, we identified a mutant with small seeds through forward genetic screening in Medicago truncatula. The mutated gene encodes MtPIN4, an ortholog of PIN1. Using molecular approaches and integrative omics analyses, we discovered that auxin and amino acid content significantly decreased in mtpin4 seeds, highlighting the role of MtPIN4-mediated auxin distribution in amino acid biosynthesis and metabolism. Furthermore, genetic analysis revealed that the three orthologs of PIN1 have specific and overlapping functions in various developmental processes in M. truncatula. Our findings emphasize the significance of MtPIN4 in seed development and offer insights into the molecular mechanisms governing the regulation of seed size in crops. This knowledge could be applied to enhance crop quality by targeted manipulation of seed protein regulatory pathways.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Key Research and Development Program of China
ID : 2023YFF1001400
Organisme : National Natural Science Foundation of China
ID : 31972958
Organisme : National Natural Science Foundation of China
ID : 32170833
Organisme : National Natural Science Foundation of China
ID : 32201446
Informations de copyright
© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.
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