Palm oil amends serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats.

DNA blood favism ovary palm oil triacylglycerol

Journal

Journal of complementary & integrative medicine

ISSN: 1553-3840

Titre abrégé: J Complement Integr Med

Pays: Germany

ID NLM: 101313855

Informations de publication

Date de publication:
06 May 2024

Historique:

received: 13 03 2024

accepted: 14 04 2024

medline: 3 5 2024

pubmed: 3 5 2024

entrez: 3 5 2024

Statut: aheadofprint

Résumé

Favism is a metabolic disease and this study evaluates the effectiveness of palm oil and its triacylglycerol constituent in favism-induced female rats to restore serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation. Animals were 36 female albino rats. They classified to two equal (normal and favism) groups. The normal group was divided into three equal subgroups: the control, palm oil, and triacylglycerol subgroups. The normal rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally, respectively. The Favism group was classified also into three equal subgroups: the favism group, the favism + palm oil, the Favism + triacylglycerol. The favism rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally. For four weeks, all treatments were administered orally via oral gavage once daily. The hemoglobin, hematocrite, the blood cells, glucose and glucose-6-phosphate dehydrogenase, and liver function were decreased in favism. Female hormones such as serum luteinizing hormone, follicle stimulating hormone, Estrone, Estriol, 17α-Estradiol, 17β-Estradiol, and Estradiol-17-β-stearate were decreased in favism. Ovarian antioxidants were decreased while ovarian inflammatory markers were increased in favism. Favism induced ovarian DNA apoptosis. Furthermore, oral administration with palm oil or its triacylglycerol constituent in favism-induced female rats restored all these parameters to be approached the control levels. Palm oil restored serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats and this effect related to oil triacylglycerol constituent.

Sections du résumé

BACKGROUND BACKGROUND

METHODS METHODS

Animals were 36 female albino rats. They classified to two equal (normal and favism) groups. The normal group was divided into three equal subgroups: the control, palm oil, and triacylglycerol subgroups. The normal rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally, respectively. The Favism group was classified also into three equal subgroups: the favism group, the favism + palm oil, the Favism + triacylglycerol. The favism rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally. For four weeks, all treatments were administered orally via oral gavage once daily.

RESULTS RESULTS

The hemoglobin, hematocrite, the blood cells, glucose and glucose-6-phosphate dehydrogenase, and liver function were decreased in favism. Female hormones such as serum luteinizing hormone, follicle stimulating hormone, Estrone, Estriol, 17α-Estradiol, 17β-Estradiol, and Estradiol-17-β-stearate were decreased in favism. Ovarian antioxidants were decreased while ovarian inflammatory markers were increased in favism. Favism induced ovarian DNA apoptosis. Furthermore, oral administration with palm oil or its triacylglycerol constituent in favism-induced female rats restored all these parameters to be approached the control levels.

CONCLUSIONS CONCLUSIONS

Palm oil restored serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats and this effect related to oil triacylglycerol constituent.

Identifiants

DOI: 10.1515/jcim-2024-0082 PMID: 38701114

pubmed: 38701114

pii: jcim-2024-0082

doi: 10.1515/jcim-2024-0082

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Informations de copyright

Références

McMillan, DC, Jollow, DJ. Favism: divicine hemotoxicity in the rat. Toxicol Sci 1999;51:310–16. https://doi.org/10.1093/toxsci/51.2.310 .

doi: 10.1093/toxsci/51.2.310

Bicakci, Z. A hemolysis trigger in glucose-6-phosphate dehydrogenase enzyme deficiency. Vicia sativa (Vetch). Saudi Med J 2009;30:292–4.

Arese, P, Mannuzzu, L, Turrini, F. Pathophysiology of favism. Folia Haematol 1989;116:745–52.

Albano, E, Tomasi, A, Mannuzzu, L, Arese, P. Detection of free radical intermediate from divicine of Vicia faba. Biochem Pharmacol 1984;33:1701–4. https://doi.org/10.1016/0006-2952(84)90299-5 .

doi: 10.1016/0006-2952(84)90299-5

Marquardt, RR. Vicine, Convicine and their role aglycnes-divine and isouramil. In: Cheeke, P, editor. Toxicants of plant origin , 2nd ed. Boca Raton, F. l., New York, USA: CRC Press; 1989:614–23 pp.

Reeves, JB, Weihrauch, JL, Consumer and Food Economics Institute . Composition of foods: fats and oils. In: Agriculture handbook 8-4. Washington, D.C.: U.S. Dept. of Agriculture, Science and Education Administration ; 1979:4 p.

Ritchie, H. Palm oil. Our world in data ; 2021.

Raghu, A. pounds of palm oil a year. Bloomberg News ; 2017. Archived from the original on 17 May 2017. Retrieved 22 May 2017.

Jadhav, HB, Annapure, US. Triglycerides of medium-chain fatty acids: a concise review. J Food Sci Technol 2023;60:2143–52. https://doi.org/10.1007/s13197-022-05499-w .

doi: 10.1007/s13197-022-05499-w

Tsimidou, MZ, Mantzouridou, FT, Nenadis, N. Minor bioactive lipids. Adv Food Nutr Res 2023;105:51–95. https://doi.org/10.1016/bs.afnr.2022.11.003 .

doi: 10.1016/bs.afnr.2022.11.003

Stavila, E, Yuliati, F, Adharis, A, Laksmono, JA, Iqbal, M. Recent advances in synthesis of polymers based on palm oil and its fatty acids. RSC Adv 2023;13:14747–75. https://doi.org/10.1039/d3ra01913f .

doi: 10.1039/d3ra01913f

Koriem, KMM. Antihyperlipidemic activity of the medicinal plants among Kadazan and Dusun communities in Sabah, Malaysia: a review. Asian Pac J Trop Biomed 2014;4:768–79. https://doi.org/10.12980/apjtb.4.2014c1144 .

doi: 10.12980/apjtb.4.2014c1144

Huneif, MA, Fahad, S, Abdulwahab, A, Alqahtani, SM, Mahnashi, MH, Nawaz, A, et al.. Antidiabetic, antihyperlipidemic, and antioxidant evaluation of phytosteroids from notholirion thomsonianum (royle) stapf. Plants 2023;12:3591. https://doi.org/10.3390/plants12203591 .

doi: 10.3390/plants12203591

Wang, H, Feng, F, Zhuang, BY, Sun, Y. Evaluation of hepatoprotective effect of Zhi-Zi-Da-Huang decoction and its two fractions against acute alcohol-induced liver injury in rats. J Ethnopharmacol 2009;126:273–9. https://doi.org/10.1016/j.jep.2009.08.028 .

doi: 10.1016/j.jep.2009.08.028

Koriem, KMM, Arbid, MSS. Evaluating of β-carotene role in ameliorating of favism-induced disturbances in blood and testis. J Compl Integr Med 2018;15:20170164. https://doi.org/10.1515/jcim-2017-0164 .

doi: 10.1515/jcim-2017-0164

Koriem, KMM, Arbid, MSS. Pickled olives neutralize vicine and convicine glycosides in Vicia faba and protect from favism. Nutr Food Sci 2021;51:959–73. https://doi.org/10.1108/nfs-10-2020-0394 .

doi: 10.1108/nfs-10-2020-0394

El-Shabrawy, OAM. Toxicological studies on Vicia faba in laboratory animals. In: M. Sc. Thesis Medical Jurisprudence . Egypt: Alexandria University; 1971:86–91 pp.

Behren, W, Karber, G. Determination of LD50. Archiv Experim Pathol Pharmacol 1953;2:177–277.

Jahn, NH. Nutritional toxicology . New York. USA: Academic Press; 1982:28–31 pp.

Uthus, EO. Diethyl maleate, an in vivo chemical depletor of glutathione, affects the response of male and female rats to arsenic deprivation. Biol Trace Elem Res 1994;46:247–59. https://doi.org/10.1007/bf02789300 .

doi: 10.1007/bf02789300

Bakhsh, A, Mustapha, NM, Mohamed, S. Catechin-rich oil palm leaf extract enhances bone calcium content of estrogen-deficient rats. Nutrition 2013;29:667–72. https://doi.org/10.1016/j.nut.2012.09.005 .

doi: 10.1016/j.nut.2012.09.005

Lisenko, KG, Andrade, EF, Lobato, RV, Orlando, DR, Damin, DH, Costa, AC, et al.. Metabolic parameters in rats receiving different levels of oral glycerol supplementation. J Anim Physiol Anim Nutr 2015;99:265–72. https://doi.org/10.1111/jpn.12217 .

doi: 10.1111/jpn.12217

Ioannou, YA, Chen, FW. Quantitation of DNA fragmentation in apoptosis. Nucleic Acids Res 1996;24:992–3. https://doi.org/10.1093/nar/24.5.992 .

doi: 10.1093/nar/24.5.992

Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning. A laboratory manual . New York: Cold Spring Harbor Laboratory, Cold spring Harbor; 1989:112–5 pp.

Stamatoyannopoulos, G, Fraser, GR, Motulsky, AG, Fessas, P, Akrivakis, A, Papayannopoulou, T. On the familial predisposition to favism. Am J Hum Genet 1966;18:253–63.

Lin, JY, LingStudies on Favism, KFLI. Isolation of an active principle from faba beans (Vicia faba L.). J Formos Med Assoc 1962;61:484–8.

Hegazy, MI, Marquardt, RR. Metabolism of vicine and convicine in rat tissues: absorption and excretion patterns and site of hydrolysis. J Sci Food Agric 1984;35:139–46. https://doi.org/10.1002/jsfa.2740350204 .

doi: 10.1002/jsfa.2740350204

Belsey, MA. The epidemiology of favism. Bull World Health Organ 1973;48:1–13.

Arbid, MSS, Marquardt, RR. Favism-like effects of divicine and isouramil in the rat: acute and chronic effects on animal health, mortalities, blood parameters and ability to exchange respiratory gases. J Sci Food Agric 1988;43:75–90. https://doi.org/10.1002/jsfa.2740430110 .

doi: 10.1002/jsfa.2740430110

Koriem, KMM, Megahed, HA, Arbid, MS. Evaluation of some adverse effects of the glycoside convicine in Sprague-Dawley rats. Toxicol Environ Chem 2008;90:415–20. https://doi.org/10.1080/02772240701483855 .

doi: 10.1080/02772240701483855

Arbid, MS, Koriem, KMM, Asaad, GF, Megahed, HA. Effect of the antibiotic neomycin on the toxicity of the glycoside vicine in rats. J Toxicol 2013;2013; 913128:1–8. https://doi.org/10.1155/2013/913128 .

doi: 10.1155/2013/913128

Marquardt, RR, Wang, N, Arbid, MSS. Pyrimidine glycosides, chapter 10. In: D’Mello, JOF, editor. Handbook of Plant and Fungal Toxicants . Boca Raton, FL, 33431, USA: CRC Press, Inc.; 1997:139–55 pp.

Szulczewska-Remi, A, Nogala-Kałucka, M, Nowak, KW. Study on the influence of palm oil on blood and liver biochemical parameters, beta-carotene and tocochromanols content as well as antioxidant activity in rats. J Food Biochem 2019;43:e12707. https://doi.org/10.1111/jfbc.12707 .

doi: 10.1111/jfbc.12707

Zhang, M, Song, G, Minuk, GY. Effects of hepatic stimulator substance, herbal medicine, selenium/vitamin E, and ciprofluoxacin on cirrhosis in the rat. Gastroenterology 1996;110:1150–5. https://doi.org/10.1053/gast.1996.v110.pm8613004 .

doi: 10.1053/gast.1996.v110.pm8613004

Koriem, KMM. Protective effect of natural products and hormones in colon cancer using metabolome: a physiological overview. Asian Pac J Trop Biomed 2017;7:957–66. https://doi.org/10.1016/j.apjtb.2017.09.002 .

doi: 10.1016/j.apjtb.2017.09.002

Beutler, E. Hemolytic anemia in disorders of red cell metabolism. In: Win- trobe, MM, editor. Topics in hematology . New York: Plenum Publishing; 1978:199–209 pp.

Koriem, K. Proteomic approach in human health and disease: preventive and cure studies. Asian Pac J Trop Biomed 2018;8:226–36. https://doi.org/10.4103/2221-1691.231285 .

doi: 10.4103/2221-1691.231285

Koriem, KMM, Arbid, MSS. Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba. Nutr Food Sci 2022;52:61–74. https://doi.org/10.1108/nfs-02-2021-0053 .

doi: 10.1108/nfs-02-2021-0053

Beretta, A, Manuelli, M, Cena, H. Favism: clinical features at different ages. Nutrients 2023;15:343. https://doi.org/10.3390/nu15020343 .

doi: 10.3390/nu15020343

Jayusman, PA, Budin, SB, Ghazali, AR, Taib, IS, Louis, SR. Effects of palm oil tocotrienol-rich fraction on biochemical and morphological alterations of liver in fenitrothion-treated rats. Pak J Pharm Sci 2014;27:1873–80.

Corchia, C, Balata, A, Meloni, GF, Meloni, T. Favism in a female newborn infant whose mother ingested fava beans before delivery. J Pediatr 1995;127:807–8. https://doi.org/10.1016/s0022-3476(95)70178-8 .

doi: 10.1016/s0022-3476(95)70178-8

Naber, EC, Vogt, H, Harnish, S, Krieg, R, Ueberschaer, KH, Rauch, HW. Reproductive performance of hens fed field beans and potential relationships to vicine metabolism. Poultry Sci 1988;67:455–62. https://doi.org/10.3382/ps.0670455 .

doi: 10.3382/ps.0670455

McIntyre, BS, Briski, KP, Tirmenstein, MA, Fariss, MW, Gapor, A, Sylvester, PW. Antiproliferative and apoptotic effects of tocopherols and tocotrienols on normal mouse mammary epithelial cells. Lipids 2000;35:171–80. https://doi.org/10.1007/bf02664767 .

doi: 10.1007/bf02664767

Saminathan, M, Mohamed, WNW, Noh, M, Ibrahim, NA, Fuat, MA, Ramiah, SK. Effects of dietary palm oil on broiler chicken productive performance and carcass characteristics: a comprehensive review. Trop Anim Health Prod 2022;54:64. https://doi.org/10.1007/s11250-022-03046-5 .

doi: 10.1007/s11250-022-03046-5

Koriem, KM, Arbid, MS, El-Gendy, NF. The protective role of anise oil in oxidative stress and genotoxicity produced in favism. J Diet Suppl 2016;13:505–21. https://doi.org/10.3109/19390211.2015.1119775 .

doi: 10.3109/19390211.2015.1119775

Koriem, KMM, Arbid, MS, Gomaa, NE. Supplementation of alpha-tocopherol attenuates minerals disturbance, oxidative stress and apoptosis occurring in favism. Indian J Clin Biochem 2017;32:446–52. https://doi.org/10.1007/s12291-016-0623-4 .

doi: 10.1007/s12291-016-0623-4

Izuddin, WI, Loh, TC, Nayan, N, Akit, H, Foo, HL, Noor, AM. Antioxidant enzyme system modulation by dietary palm oils, palm kernel oil and soybean oil in laying hens. Animals 2023;13:2245. https://doi.org/10.3390/ani13142245 .

doi: 10.3390/ani13142245

Teh, SS, Mah, SH, Lau, HLN, Teng, KT, Loganathan, R. Antioxidant potential of red palm-pressed mesocarp olein. J Oleo Sci 2021;70:1719–29. https://doi.org/10.5650/jos.ess21147 .

doi: 10.5650/jos.ess21147

Zainal, Z, Khaza’ai, H, Radhakrishnan, AK, Chang, SK. Therapeutic potential of palm oil vitamin E-derived tocotrienols in inflammation and chronic diseases: evidence from preclinical and clinical studies. Food Res Int 2022;156:111175. https://doi.org/10.1016/j.foodres.2022.111175 .

doi: 10.1016/j.foodres.2022.111175

Abdulkarim, MF, Abdullah, GZ, Chitneni, M, Salman, IM, Ameer, OZ, Yam, MF, et al.. Topical piroxicam in vitro release and in vivo anti-inflammatory and analgesic effects from palm oil esters-based nanocream. Int J Nanomed 2010;5:915–24. https://doi.org/10.2147/ijn.s13305 .

doi: 10.2147/ijn.s13305