Stress phenotyping analysis leveraging autofluorescence image sequences with machine learning.

Brassica rapa autofluorescence imaging drought stress genotypic variation high throughput plant phenotyping machine learning-based classifier stress detection

Journal

Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200

Informations de publication

Date de publication:
2024
Historique:
received: 09 12 2023
accepted: 14 03 2024
medline: 6 5 2024
pubmed: 6 5 2024
entrez: 6 5 2024
Statut: epublish

Résumé

Autofluorescence-based imaging has the potential to non-destructively characterize the biochemical and physiological properties of plants regulated by genotypes using optical properties of the tissue. A comparative study of stress tolerant and stress susceptible genotypes of Autofluorescence spectral images have been used to design a stress detection classifier with two classes, stressed and non-stressed, using machine learning algorithms. The benchmark dataset consisted of time-series image sequences from three The study demonstrated that both the computed phenotypes consistently discriminated against stressed Autofluorescence signals from the 365/400 nm excitation/emission combination were able to segregate genotypic variation during a progressive drought treatment under a controlled greenhouse environment, allowing for the exploration of other meaningful phenotypes using autofluorescence image sequences with significance in the context of plant science.

Sections du résumé

Background UNASSIGNED
Autofluorescence-based imaging has the potential to non-destructively characterize the biochemical and physiological properties of plants regulated by genotypes using optical properties of the tissue. A comparative study of stress tolerant and stress susceptible genotypes of
Methods UNASSIGNED
Autofluorescence spectral images have been used to design a stress detection classifier with two classes, stressed and non-stressed, using machine learning algorithms. The benchmark dataset consisted of time-series image sequences from three
Results UNASSIGNED
The study demonstrated that both the computed phenotypes consistently discriminated against stressed
Conclusion UNASSIGNED
Autofluorescence signals from the 365/400 nm excitation/emission combination were able to segregate genotypic variation during a progressive drought treatment under a controlled greenhouse environment, allowing for the exploration of other meaningful phenotypes using autofluorescence image sequences with significance in the context of plant science.

Identifiants

pubmed: 38708393
doi: 10.3389/fpls.2024.1353110
pmc: PMC11066247
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1353110

Informations de copyright

Copyright © 2024 Das Choudhury, Guadagno, Bashyam, Mazis, Ewers, Samal and Awada.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Auteurs

Sruti Das Choudhury (S)

School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.
School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.

Carmela Rosaria Guadagno (CR)

Department of Botany, University of Wyoming, Laramie, WY, United States.

Srinidhi Bashyam (S)

School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.

Anastasios Mazis (A)

School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.

Brent E Ewers (BE)

Department of Botany, University of Wyoming, Laramie, WY, United States.

Ashok Samal (A)

School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.

Tala Awada (T)

School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.
Agricultural Research Division, University of Nebraska-Lincoln, Lincoln, NE, United States.

Classifications MeSH