A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells.

Actins / metabolism Humans Magnetic Fields Actin Cytoskeleton / metabolism Microspheres Animals
Actin cortex Cell mechanics Dynamic measurement Image analysis Magnetic beads

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2024
Historique:
medline: 6 5 2024
pubmed: 6 5 2024
entrez: 6 5 2024
Statut: ppublish

Résumé

The actin cortex is an essential element of the cytoskeleton allowing cells to control and modify their shape. It is involved in cell division and migration. However, probing precisely the physical properties of the actin cortex has proved to be challenging: it is a thin and dynamic material, and its location in the cell-directly under the plasma membrane-makes it difficult to study with standard light microscopy and cell mechanics techniques. In this chapter, we present a novel protocol to probe dynamically the thickness of the cortex and its fluctuations using superparamagnetic microbeads in a uniform magnetic field. A bead ingested by the cell and another outside the cell attract each other due to dipolar forces. By tracking their position with nanometer precision, one can measure the thickness of the cortex pinched between two beads and monitor its evolution in time. We first present the set of elements necessary to realize this protocol: a magnetic field generator adapted to a specific imaging setup and the aforementioned superparamagnetic microbeads. Then we detail the different steps of a protocol that can be used on diverse cell types, adherent or not.

Identifiants

DOI: 10.1007/978-1-0716-3834-7_10 PMID: 38709482
pubmed: 38709482
doi: 10.1007/978-1-0716-3834-7_10
doi:

Substances chimiques

Actins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

115-145

Informations de copyright

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Joseph Vermeil (J)

Physique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.
UMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France.

Valentin Laplaud (V)

LadHyX, CNRS, Ecole polytechnique, Institut Polytechnique de Paris, Palaiseau, France.

Anumita Jawahar (A)

Physique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.
UMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France.

Dulamkhuu Bujaa (D)

Physique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Damien Cuvelier (D)

UMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France.

Julien Heuvingh (J)

Physique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Olivia du Roure (O)

Physique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Matthieu Piel (M)

UMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France. matthieu.piel@curie.fr.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Actines › A Magnetic Pincher for the Dynamic Measurement...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A Magnetic Pincher for the Dynamic Measurement...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Champs magnétiques › A Magnetic Pincher for the Dynamic Measurement...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › A Magnetic Pincher for the Dynamic Measurement...
questionsmedicales.fr › Équipement et fournitures › Microsphères › A Magnetic Pincher for the Dynamic Measurement...
questionsmedicales.fr › Eucaryotes › Animaux › A Magnetic Pincher for the Dynamic Measurement...