The relaxation effect of endocannabinoid anandamide on isolated rat bladder and vas deferens tissues and possible mechanisms.
anandamide
rat bladder
rat vas deferens
smooth muscle relaxation responses
Journal
Fundamental & clinical pharmacology
ISSN: 1472-8206
Titre abrégé: Fundam Clin Pharmacol
Pays: England
ID NLM: 8710411
Informations de publication
Date de publication:
07 May 2024
07 May 2024
Historique:
revised:
17
04
2024
received:
31
10
2023
accepted:
24
04
2024
medline:
8
5
2024
pubmed:
8
5
2024
entrez:
8
5
2024
Statut:
aheadofprint
Résumé
The endocannabinoid system plays important roles in various systems, including the genitourinary system; however, its mechanism of action is not fully understood. This study aimed to investigate the direct relaxant effects of anandamide and its possible mechanisms in isolated rat bladder and vas deferens tissues. Twenty-one adult male Wistar albino rats were used. Bladder and vas deferens (prostatic and epididymal portions) tissues were mounted in 10 mL of organ baths. Relaxation responses to anandamide were recorded at 3 and 10 μM concentrations. After the rest period, the procedures were repeated in the presence of cannabinoid (CB) and vanilloid receptor antagonists, various potassium channel blockers, cyclo-oxygenase, and nitric oxide synthase inhibitors. In different tissues to investigate the Ca Anandamide caused a significant relaxation response in the bladder and epididymal vas deferens tissues, but not in the prostatic portion. The effect of anandamide was antagonized in the presence of the CB The results show that anandamide has a direct relaxant effect on the isolated rat bladder and epididymal vas deferens. Anandamide triggers different mechanisms in different types of tissues, and further studies are needed to elucidate the mechanism of action of anandamide.
Sections du résumé
BACKGROUND
BACKGROUND
The endocannabinoid system plays important roles in various systems, including the genitourinary system; however, its mechanism of action is not fully understood.
OBJECTIVES
OBJECTIVE
This study aimed to investigate the direct relaxant effects of anandamide and its possible mechanisms in isolated rat bladder and vas deferens tissues.
METHODS
METHODS
Twenty-one adult male Wistar albino rats were used. Bladder and vas deferens (prostatic and epididymal portions) tissues were mounted in 10 mL of organ baths. Relaxation responses to anandamide were recorded at 3 and 10 μM concentrations. After the rest period, the procedures were repeated in the presence of cannabinoid (CB) and vanilloid receptor antagonists, various potassium channel blockers, cyclo-oxygenase, and nitric oxide synthase inhibitors. In different tissues to investigate the Ca
RESULTS
RESULTS
Anandamide caused a significant relaxation response in the bladder and epididymal vas deferens tissues, but not in the prostatic portion. The effect of anandamide was antagonized in the presence of the CB
CONCLUSIONS
CONCLUSIONS
The results show that anandamide has a direct relaxant effect on the isolated rat bladder and epididymal vas deferens. Anandamide triggers different mechanisms in different types of tissues, and further studies are needed to elucidate the mechanism of action of anandamide.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Sağlık Bilimleri Üniversitesi
ID : 2022/159
Informations de copyright
© 2024 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd.
Références
Aizpurua‐Olaizola O, Elezgarai I, Rico‐Barrio I, Zarandona I, Etxebarria N, Usobiaga A. Targeting the endocannabinoid system: future therapeutic strategies. Drug Discov Today. 2017;22(1):105‐110. doi:10.1016/j.drudis.2016.08.005
Lu H‐C, Mackie K. Review of the endocannabinoid system. Biol Psychiatry: Cogn Neurosci Neuroimaging. 2021;6(6):607‐615. doi:10.1016/j.bpsc.2020.07.016
Di Marzo V. New approaches and challenges to targeting the endocannabinoid system. Nat Rev Drug Discov. 2018;17(9):623‐639. doi:10.1038/nrd.2018.115
Di Marzo V, Wang J. The Endocannabinoidome: The World of Endocannabinoids and Related Mediators. Academic Press; 2014.
Howlett AC, Abood ME. CB1 and CB2 receptor pharmacology. Adv Pharmacol. 2017;80:169‐206. doi:10.1016/bs.apha.2017.03.007
Wheal AJ, Alexander SPH, Randall MD. Vasorelaxation to N‐oleoylethanolamine in rat isolated arteries: mechanisms of action and modulation via cyclooxygenase activity. Br J Pharmacol. 2010;160(3):701‐711. doi:10.1111/j.1476‐5381.2010.00770.x
Randall MD, Kendall DA, O'Sullivan S. The complexities of the cardiovascular actions of cannabinoids. Br J Pharmacol. 2004;142(1):20‐26. doi:10.1038/sj.bjp.0705725
Vanessa Ho WS, Hiley CR. Endothelium‐independent relaxation to cannabinoids in rat‐isolated mesenteric artery and role of Ca2+ influx. Br J Pharmacol. 2003;139(3):585‐597. doi:10.1038/sj.bjp.0705280
Lim J, Squire E, Jung K‐M. Phytocannabinoids, the endocannabinoid system and male reproduction. World J Mens Health. 2023;41(1):1‐10. doi:10.5534/wjmh.220132
Sayed TS, Balasinor NH, Nishi K. Diverse role of endocannabinoid system in mammalian male reproduction. Life Sci. 2021;286:120035. doi:10.1016/j.lfs.2021.120035
Kim SD, Cho KJ, Kim JC. Expression of cannabinoid 1 and, 2 receptors and the effects of cannabinoid 1 and, 2 receptor agonists on detrusor overactivity associated with bladder outlet obstruction in rats. BMC Urol. 2017;17(1):121. doi:10.1186/s12894‐017‐0313‐4
Cristino L, Bisogno T, Di Marzo V. Cannabinoids and the expanded endocannabinoid system in neurological disorders. Nat Rev Neurol. 2020;16(1):9‐29. doi:10.1038/s41582‐019‐0284‐z
Sultana S, Berger G, Lehmann C. Components of the endogenous cannabinoid system as potential biomarkers for interstitial cystitis/bladder pain syndrome. Diagnostics. 2021;12(1):19. doi:10.3390/diagnostics12010019
Al‐Hayani A, Wease KN, Ross RA, Pertwee RG, Davies SN. The endogenous cannabinoid anandamide activates vanilloid receptors in the rat hippocampal slice. Neuropharmacology. 2001;41(8):1000‐1005. doi:10.1016/S0028‐3908(01)00145‐9
Ross RA, Gibson TM, Brockie HC, et al. Structure‐activity relationship for the endogenous cannabinoid, anandamide, and certain of its analogues at vanilloid receptors in transfected cells and vas deferens. Br J Pharmacol. 2001;132(3):631‐640. doi:10.1038/sj.bjp.0703850
Seyrek M, Irkilata HC, Vural IM, et al. Testosterone relaxes human internal spermatic vein through potassium channel opening action. Urology. 2011;78(1):233.e1‐233.e5. doi:10.1016/j.urology.2011.03.003
Lobato NS, Filgueira FP, Prakash R, et al. Reduced endothelium‐dependent relaxation to anandamide in mesenteric arteries from young obese Zucker rats. PLoS ONE. 2013;8(5):e63449. doi:10.1371/journal.pone.0063449
Peroni RN, Abramoff T, Neuman I, Podestá EJ, Adler‐Graschinsky E. Phytoestrogens enhance the vascular actions of the endocannabinoid anandamide in mesenteric beds of female rats. Int. J Hypertens. 2012;2012:647856. doi:10.1155/2012/647856
Asala AK, Diab AA, Atia KI, Fatthy MA. Cannabinoid induced changes in rat uterine contractility. 2013:19.
Bonz A, Laser M, Küllmer S, et al. Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle. J Cardiovasc Pharmacol. 2003;41(4):657‐664. doi:10.1097/00005344‐200304000‐00020
Bakali E, Elliott RA, Taylor AH, Willets J, Konje JC, Tincello DG. Distribution and function of the endocannabinoid system in the rat and human bladder. Int Urogynecol J Pelvic Floor Dysfunct. 2013;24(5):855‐863. doi:10.1007/s00192‐012‐1954‐1
Hayn MH, Ballesteros I, de Miguel F, et al. Functional and immunohistochemical characterization of CB1 and CB2 receptors in rat bladder. Urology. 2008;72(5):1174‐1178. doi:10.1016/j.urology.2008.03.044
Hiragata S, Ogawa T, Hayashi Y, et al. Effects of IP‐751, ajulemic acid, on bladder overactivity induced by bladder irritation in rats. Urology. 2007;70(1):202‐208. doi:10.1016/j.urology.2007.02.069
Elgohary R, Salama A, Omara EA. Protective effects of Cannabis sativa on chemotherapy‐induced nausea in a rat: involvement of CB1 receptors. Fundam Clin Pharmacol. 2023;37(1):37‐146. doi:10.1111/fcp.12821
Baker SA. Molecular and Functional Identification of the Two‐Pore Potassium Ion Channels in Bladder Smooth Muscle. University of Nevada; 2008.
Dogan MF, Yildiz O, Arslan SO, Ulusoy KG. Potassium channels in vascular smooth muscle: a pathophysiological and pharmacological perspective. Fundam Clin Pharmacol. 2019;33(5):504‐523. doi:10.1111/fcp.12461
Langton PD, Nelson MT, Huang Y, Standen NB. Block of calcium‐activated potassium channels in mammalian arterial myocytes by tetraethylammonium ions. Am J Physiol Heart Circ Physiol. 1991;260(3):H927. doi:10.1152/ajpheart.1991.260.3.h927
Luzhkov VB, Åqvist J. Mechanisms of tetraethylammonium ion block in the KcsA potassium channel. FEBS Lett. 2001;495(3):191‐196. doi:10.1016/S0014‐5793(01)02381‐X
Nagao T, Vanhoutte PM. Endothelium‐derived hyperpolarizing factor and endothelium‐dependent relaxations. Am J Respir Cell Mol Biol. 1993;8:1‐6. doi:10.1165/ajrcmb/8.1.1
White R, Hiley CR. A comparison of EDHF‐mediated and anandamide‐induced relaxations in the rat isolated mesenteric artery. Br J Pharmacol. 1997;122(8):1573‐1584. doi:10.1038/sj.bjp.0701546
White R, Vanessa Ho WSV, Bottrill FE, Ford WR, Hiley CR. Mechanisms of anandamide‐induced vasorelaxation in rat isolated coronary arteries. Br J Pharmacol. 2001;134(4):921‐929. doi:10.1038/sj.bjp.0704333
Grainger J, Boachie‐Ansah G. Anandamide‐induced relaxation of sheep coronary arteries: the role of the vascular endothelium, arachidonic acid metabolites and potassium channels. Br J Pharmacol. 2001;134(5):1003‐1012. doi:10.1038/sj.bjp.0704340
Barun S, Vural IM, Dileköz E, Ercan ZS, Sarioglu Y. Effects of cannabinoid receptor activation on rabbit bisected vas deferens strips. Clin Exp Pharmacol Physiol. 2005;32(9):702‐707. doi:10.1111/j.1440‐1681.2005.04261.x
Pertwee RG, Fernando SR, Griffin G, Abadji V, Makriyannis A. Effect of phenylmethylsulphonyl fluoride on the potency of anandamide as an inhibitor of electrically evoked contractions in two isolated tissue preparations. Eur J Pharmacol. 1995;272(1):73‐78. doi:10.1016/0014‐2999(94)00618‐H
Mang CF, Erbelding D, Kilbinger H. Differential effects of anandamide on acetylcholine release in the guinea‐pig ileum mediated via vanilloid and non‐CB1 cannabinoid receptors. Br J Pharmacol. 2001;134(1):161‐167. doi:10.1038/sj.bjp.0704220
Fowler CJ. The cannabinoid system and its pharmacological manipulation—a review, with emphasis upon the uptake and hydrolysis of anandamide. Fundam Clin Pharmacol. 2006;20(6):549‐562. doi:10.1111/j.1472‐8206.2006.00442.x
Smid SD, Bjorklund CK, Svensson KM, Heigis S, Revesz A. The endocannabinoids anandamide and 2‐arachidonoylglycerol inhibit cholinergic contractility in the human colon. Eur J Pharmacol. 2007;575(1‐3):168‐176. doi:10.1016/j.ejphar.2007.07.036
Kulesza B, Mazurek M, Kurzepa J. Can cannabidiol have an analgesic effect? Fundam Clin Pharmacol. 2024;38(1):33‐41. doi:10.1111/fcp.12947
Patsos HA, Greenhough A, Hicks DJ, et al. The endogenous cannabinoid, anandamide, induces COX‐2‐dependent cell death in apoptosis‐resistant colon cancer cells. Int J Oncol. 2010;37(1):187‐193. doi:10.3892/ijo_00000666
Maccarrone M, Bari M, Lorenzon T, Bisogno T, Di Marzo V, Finazzi‐Agrò A. Anandamide uptake by human endothelial cells and its regulation by nitric oxide. J Biol Chem. 2000;275(18):13484‐13492. doi:10.1074/jbc.275.18.13484
Poblete IM, Orliac ML, Briones R, Adler‐Graschinsky E, Huidobro‐Toro JP. Anandamide elicits an acute release of nitric oxide through endothelial TRPV1 receptor activation in the rat arterial mesenteric bed. J Physiol. 2005;568(2):539‐551. doi:10.1113/jphysiol.2005.094292
Someya A, Horie S, Murayama T. Arachidonic acid release and prostaglandin F2α formation induced by anandamide and capsaicin in PC12 cells. Eur J Pharmacol. 2002;450(2):131‐139. doi:10.1016/S0014‐2999(02)02122‐2
Sordelli MS, Beltrame JS, Cella M, Franchi AM, Ribeiro ML. Cyclooxygenase‐2 prostaglandins mediate anandamide‐inhibitory action on nitric oxide synthase activity in the receptive rat uterus. Eur J Pharmacol. 2012;685(1‐3):174‐179. doi:10.1016/j.ejphar.2012.04.034
Vural EH, Ozturk Fincan GS, Okcay Y, Askin CI, Gudul Bacanli M, Vural IM. Interaction of endocannabinoid system and cyclooxygenase metabolites with fatty acid amide hydrolase and cyclooxygenase enzyme activities on contractile responses in rat vas deferens tissue. Naunyn Schmiedebergs Arch Pharmacol. 2023. doi:10.1007/s00210‐023‐02861‐3
Ching LC, Kou YR, Shyue SK, et al. Molecular mechanisms of activation of endothelial nitric oxide synthase mediated by transient receptor potential vanilloid type 1. Cardiovasc Res. 2011;91(3):492‐501. doi:10.1093/cvr/cvr104
Breese NM, George AC, Pauers LE, Stucky CL. Peripheral inflammation selectively increases TRPV1 function in IB4‐positive sensory neurons from adult mouse. Pain. 2005;115(1):37‐49. doi:10.1016/j.pain.2005.02.010
Dinis P, Charrua A, Avelino A, et al. Anandamide‐evoked activation of vanilloid receptor 1 contributes to the development of bladder hyperreflexia and nociceptive transmission to spinal dorsal horn neurons in cystitis. J Neurosci. 2004;24(50):11253‐11263. doi:10.1523/JNEUROSCI.2657‐04.2004
Osycka‐Salut C, Gervasi MG, Pereyra E, et al. Anandamide induces sperm release from oviductal epithelia through nitric oxide pathway in bovines. PLoS ONE. 2012;7(2):e30671. doi:10.1371/journal.pone.0030671
Stromberga Z, Chess‐Williams R, Moro C. Prostaglandin E2 and F2α modulate urinary bladder urothelium, lamina propria and detrusor contractility via the FP receptor. Front Physiol. 2020;11:508789. doi:10.3389/fphys.2020.00705
Saitoh C, Kitada C, Uchida W, Chancellor MB, de Groat WC, Yoshimura N. The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Eur J Pharmacol. 2007;570(1‐3):182‐187. doi:10.1016/j.ejphar.2007.05.016
Ross RA, Craib SJ, Stevenson LA, et al. Pharmacological characterization of the anandamide cyclooxygenase metabolite: prostaglandin E2 ethanolamide. J Pharmacol Exp Ther. 2002;301(3):900‐907. doi:10.1124/jpet.301.3.900
Yu M, Ives D, Ramesha CS. Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase‐2. J Biol Chem. 1997;272(34):21181‐21186. doi:10.1074/jbc.272.34.21181
Kozak KR, Crews BC, Morrow JD, et al. Metabolism of the endocannabinoids, 2‐arachidonylglycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides. J Biol Chem. 2002;277(47):44877‐44885. doi:10.1074/jbc.M206788200
Van Der Stelt M, Di Marzo V. Anandamide as an intracellular messenger regulating ion channel activity. Prostaglandins Lipid Mediat. 2005;77(1‐4):111‐122. doi:10.1016/j.prostaglandins.2004.09.007
Van der Stelt M, Trevisani M, Vellani V, et al. Anandamide acts as an intracellular messenger amplifying Ca2+ influx via TRPV1 channels. EMBO j. 2005;24(17):3026‐3037. doi:10.1038/sj.emboj.7600784
Fimiani C, Mattocks D, Cavani F, et al. Morphine and anandamide stimulate intracellular calcium transients in human arterial endothelial cells: coupling to nitric oxide release. Cell Signal. 1999;11(3):189‐193. doi:10.1016/S0898‐6568(98)00060‐6
Fenwick AJ, Fowler DK, Wu SW, et al. Direct anandamide activation of TRPV1 produces divergent calcium and current responses. Front Mol Neurosci. 2017;10:200. doi:10.3389/fnmol.2017.00200