Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection.
Humans
Hepatic Stellate Cells
/ metabolism
HIV Infections
/ complications
Coinfection
Hepacivirus
/ physiology
Hepatitis C
/ metabolism
Coculture Techniques
Jurkat Cells
Liver Cirrhosis
/ metabolism
Cytokines
/ metabolism
Bystander Effect
Hepatocytes
/ metabolism
HIV
/ physiology
Oxidative Stress
Cell Communication
CD4-Positive T-Lymphocytes
/ immunology
Extracellular Matrix
/ metabolism
Journal
Journal of immunology research
ISSN: 2314-7156
Titre abrégé: J Immunol Res
Pays: Egypt
ID NLM: 101627166
Informations de publication
Date de publication:
2024
2024
Historique:
received:
15
02
2024
revised:
10
04
2024
accepted:
17
04
2024
medline:
8
5
2024
pubmed:
8
5
2024
entrez:
8
5
2024
Statut:
epublish
Résumé
This study aims to explore the influence of coinfection with HCV and HIV on hepatic fibrosis. A coculture system was set up to actively replicate both viruses, incorporating CD4 T lymphocytes (Jurkat), hepatic stellate cells (LX-2), and hepatocytes (Huh7.5). LX-2 cells' susceptibility to HIV infection was assessed through measurements of HIV receptor expression, exposure to cell-free virus, and cell-to-cell contact with HIV-infected Jurkat cells. The study evaluated profibrotic parameters, including programed cell death, ROS imbalance, cytokines (IL-6, TGF-
Identifiants
pubmed: 38715844
doi: 10.1155/2024/6343757
pmc: PMC11074826
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6343757Informations de copyright
Copyright © 2024 Cintia Cevallos et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interests.