Basophil allergen threshold sensitivity to casein (casein-specific CD-sens) predicts allergic reactions at a milk challenge in most but not all patients.
CD‐sens
basophil activation
casein
cow's milk allergy
food challenge
Journal
Immunity, inflammation and disease
ISSN: 2050-4527
Titre abrégé: Immun Inflamm Dis
Pays: England
ID NLM: 101635460
Informations de publication
Date de publication:
May 2024
May 2024
Historique:
revised:
02
04
2024
received:
20
10
2023
accepted:
18
04
2024
medline:
9
5
2024
pubmed:
9
5
2024
entrez:
9
5
2024
Statut:
ppublish
Résumé
The basophil activation test is an emerging clinical tool in the diagnosis of cow's milk allergy (CMA). The aim was to assess the association between the basophil allergen threshold sensitivity to the major milk protein casein (casein-specific CD-sens), the levels of milk- and casein-specific Immunoglobulin E antibodies (IgE-ab), and the severity of allergic reactions at milk challenges. We enrolled 34 patients aged 5-15 (median 9) years who underwent a double-blind placebo-controlled milk-challenge (DBPCMC) as screening before inclusion in an oral immunotherapy study for CMA. The severity of the allergic reaction at the DBPCMC was graded using Sampson's severity score. Venous blood was drawn before the DBPCMC. Milk- and casein-specific IgE-ab were analyzed. Following in vitro stimulation of basophils with casein, casein-specific CD-sens, was determined. Thirty-three patients completed the DBPCMC. There were strong correlations between casein-specific CD-sens and IgE-ab to milk (r Two thirds of those reacting at the DBPMC had positive casein-specific CD-sens, but reactions also occurred despite negative casein-specific CD-sens. The association between casein-specific CD-sens and the severity of the allergic reaction and cumulative dose of milk protein, respectively, was moderate.
Sections du résumé
BACKGROUND
BACKGROUND
The basophil activation test is an emerging clinical tool in the diagnosis of cow's milk allergy (CMA). The aim was to assess the association between the basophil allergen threshold sensitivity to the major milk protein casein (casein-specific CD-sens), the levels of milk- and casein-specific Immunoglobulin E antibodies (IgE-ab), and the severity of allergic reactions at milk challenges.
METHODS
METHODS
We enrolled 34 patients aged 5-15 (median 9) years who underwent a double-blind placebo-controlled milk-challenge (DBPCMC) as screening before inclusion in an oral immunotherapy study for CMA. The severity of the allergic reaction at the DBPCMC was graded using Sampson's severity score. Venous blood was drawn before the DBPCMC. Milk- and casein-specific IgE-ab were analyzed. Following in vitro stimulation of basophils with casein, casein-specific CD-sens, was determined.
RESULTS
RESULTS
Thirty-three patients completed the DBPCMC. There were strong correlations between casein-specific CD-sens and IgE-ab to milk (r
CONCLUSION
CONCLUSIONS
Two thirds of those reacting at the DBPMC had positive casein-specific CD-sens, but reactions also occurred despite negative casein-specific CD-sens. The association between casein-specific CD-sens and the severity of the allergic reaction and cumulative dose of milk protein, respectively, was moderate.
Substances chimiques
Caseins
0
Immunoglobulin E
37341-29-0
Allergens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1265Subventions
Organisme : Ellen, Walter and Lennart Hesselman Foundation for Scientific Research
Organisme : Västerbotten Läns Landsting
ID : RV 832 441
Organisme : Västerbotten Läns Landsting
ID : RV 864 981
Organisme : Västerbotten Läns Landsting
ID : RV 941 767
Organisme : Västerbotten Läns Landsting
ID : RV 969 865
Organisme : Västerbotten Läns Landsting
ID : RV932 940
Organisme : VISARENORR
ID : 851 551
Organisme : VISARENORR
ID : 930736
Organisme : Jämtland/Härjedalen County Council
ID : JLL-559111
Organisme : Jämtland/Härjedalen County Council
ID : JLL-652181
Organisme : Jämtland/Härjedalen County Council
ID : JLL-738681
Organisme : Jämtland/Härjedalen County Council
ID : JLL-851571
Organisme : Jämtland/Härjedalen County Council
ID : JLL-930965
Organisme : Jämtland/Härjedalen County Council
ID : JLL-939570
Organisme : Jämtland/Härjedalen County Council
ID : JLL-968247
Organisme : Swedish Asthma and Allergy Foundation
Organisme : Stockholm läns landsting
ID : RS 2019-1054
Organisme : Insamlingsstiftelsen Cancer- och Allergifonden
Informations de copyright
© 2024 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.
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