Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages.

Point Mutation Capsid / metabolism Capsid Proteins / genetics Cryoelectron Microscopy Myxococcus xanthus / genetics Models, Molecular
HK97 encapsulin protein cage self-assembly viral capsid

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
14 May 2024
Historique:
medline: 10 5 2024
pubmed: 10 5 2024
entrez: 9 5 2024
Statut: ppublish

Résumé

Protein capsids are a widespread form of compartmentalization in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximizes the internal volume that can be enclosed within. Despite the strong preference for icosahedral symmetry, we show that simple point mutations in a virus-like capsid can drive the assembly of unique symmetry-reduced structures. Starting with the encapsulin from

Identifiants

DOI: 10.1073/pnas.2321260121 PMID: 38722807
pubmed: 38722807
doi: 10.1073/pnas.2321260121
doi:

Substances chimiques

Capsid Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2321260121

Subventions

Organisme : Wellcome Trust
ID : 110145
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 110146
Pays : United Kingdom

Déclaration de conflit d'intérêts

Competing interests statement:Two of the authors (B.E.D. and M.F.J.) are shareholders in Megadalton Solutions, a company that is engaged in commercializing CDMS. B.E.D. is an employee of Megadalton Solutions and M.F.J. is a consultant for Waters.

Auteurs

Taylor N Szyszka (TN)

School of Chemistry, The University of Sydney, Camperdown, NSW 2006, Australia.
The University of Sydney Nano Institute, The University of Sydney, Camperdown, NSW 2006, Australia.
ORCID: 0000-0002-9921-3086

Michael P Andreas (MP)

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109.

Felicia Lie (F)

School of Chemistry, The University of Sydney, Camperdown, NSW 2006, Australia.

Lohra M Miller (LM)

Chemistry Department, Indiana University, Bloomington, IN 47405.

Lachlan S R Adamson (LSR)

School of Chemistry, The University of Sydney, Camperdown, NSW 2006, Australia.

Farzad Fatehi (F)

Department of Mathematics, University of York, York YO10 5DD, United Kingdom.
York Cross-Disciplinary Centre for Systems Analysis, University of York, York YO10 5DD, United Kingdom.
ORCID: 0000-0003-3743-5772

Reidun Twarock (R)

Department of Mathematics, University of York, York YO10 5DD, United Kingdom.
York Cross-Disciplinary Centre for Systems Analysis, University of York, York YO10 5DD, United Kingdom.
Department of Biology, University of York, York YO10 5DD, United Kingdom.
ORCID: 0000-0002-1824-2003

Benjamin E Draper (BE)

Megadalton Solutions Inc., Bloomington, IN 47401.

Martin F Jarrold (MF)

Chemistry Department, Indiana University, Bloomington, IN 47405.

Tobias W Giessen (TW)

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109.

Yu Heng Lau (YH)

School of Chemistry, The University of Sydney, Camperdown, NSW 2006, Australia.
The University of Sydney Nano Institute, The University of Sydney, Camperdown, NSW 2006, Australia.
ORCID: 0000-0001-6560-8410

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mutation ponctuelle › Point mutation in a virus-like capsid drives...
questionsmedicales.fr › Structures virales › Virion › Nucléocapside › Capside › Point mutation in a virus-like capsid drives...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines de capside › Point mutation in a virus-like capsid drives...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Point mutation in a virus-like capsid drives...
questionsmedicales.fr › Bactéries › Proteobacteria › Deltaproteobacteria › Myxococcales › Myxococcus › Myxococcus xanthus › Point mutation in a virus-like capsid drives...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Point mutation in a virus-like capsid drives...