Zooming into lipid droplet biology through the lens of electron microscopy.
CLEM
FIB‐SEM
cellular ultrastructure
cryogenic electron microscopy
cryo‐ET
cryo‐FIB
macromolecular structure
transmission electron microscopy
vitrification
volume imaging
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
10 May 2024
10 May 2024
Historique:
revised:
08
04
2024
received:
26
01
2024
accepted:
24
04
2024
medline:
10
5
2024
pubmed:
10
5
2024
entrez:
10
5
2024
Statut:
aheadofprint
Résumé
Electron microscopy (EM), in its various flavors, has significantly contributed to our understanding of lipid droplets (LD) as central organelles in cellular metabolism. For example, EM has illuminated that LDs, in contrast to all other cellular organelles, are uniquely enclosed by a single phospholipid monolayer, revealed the architecture of LD contact sites with different organelles, and provided near-atomic resolution maps of key enzymes that regulate neutral lipid biosynthesis and LD biogenesis. In this review, we first provide a brief history of pivotal findings in LD biology unveiled through the lens of an electron microscope. We describe the main EM techniques used in the context of LD research and discuss their current capabilities and limitations, thereby providing a foundation for utilizing suitable EM methodology to address LD-related questions with sufficient level of structural preservation, detail, and resolution. Finally, we highlight examples where EM has recently been and is expected to be instrumental in expanding the frontiers of LD biology.
Identifiants
pubmed: 38726814
doi: 10.1002/1873-3468.14899
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Molecular Biology Laboratory
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 01028297
Organisme : Biomedicum Helsinki Foundation
Organisme : Orion Research Foundation
Organisme : Finnish Cultural Foundation
Informations de copyright
© 2024 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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