Optimization studies on laccase activity of Proteus mirabilis isolated from treatment sludge of textile industry factories : Optimization of laccase activity of Proteus mirabilis.

Proteus mirabilis Bacterial laccase Design of experiment One factor at a time Optimization Response surface method (RSM)

Journal

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924

Informations de publication

Date de publication:
10 May 2024
Historique:
received: 06 12 2023
accepted: 18 04 2024
medline: 10 5 2024
pubmed: 10 5 2024
entrez: 10 5 2024
Statut: aheadofprint

Résumé

Laccase is an exothermic enzyme with copper in its structure and has an important role in biodegradation by providing oxidation of phenolic compounds and aromatic amines and decomposing lignin. The aim of this study is to reach maximum laccase enzyme activity with minimum cost and energy through optimization studies of Proteusmirabilis isolated from treatment sludge of a textile factory. In order to increase the laccase enzyme activities of the isolates, medium and culture conditions were optimized with the study of carbon (Glucose, Fructose, Sodium Acetate, Carboxymethylcellulose, Xylose) and nitrogen sources (Potassium nitrate, Yeast Extract, Peptone From Soybean, Bacteriological Peptone), incubation time, pH, temperature and Copper(II) sulfate concentration then according to the results obtained. Response Surface Method (RSM) was performed on six different variables with three level. According to the data obtained from the RSM, the maximum laccase enzyme activity is reached at pH 7.77, temperature 30.03

Identifiants

pubmed: 38727921
doi: 10.1007/s42770-024-01350-w
pii: 10.1007/s42770-024-01350-w
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Eskişehir Osmangazi Üniversitesi
ID : FYL_2021-2210

Informations de copyright

© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.

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Auteurs

Kübra Oztat (K)

Eskisehir Osmangazi University, Eskişehir, Türkiye. kocakkubra1995@gmail.com.

Arzu Altın Yavuz (AA)

Eskisehir Osmangazi University, Eskişehir, Türkiye.

Cansu Filik Işçen (CF)

Eskisehir Osmangazi University, Eskişehir, Türkiye. cfilik@ogu.edu.tr.

Classifications MeSH