An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis.
Parasteatoda tepidariorum
Cell atlas
Development
Extra-embryonic
Head patterning
Hox genes
Segmentation
Single-cell RNA sequencing
Spider
Journal
EvoDevo
ISSN: 2041-9139
Titre abrégé: Evodevo
Pays: England
ID NLM: 101525836
Informations de publication
Date de publication:
10 May 2024
10 May 2024
Historique:
received:
10
07
2023
accepted:
15
04
2024
medline:
11
5
2024
pubmed:
11
5
2024
entrez:
10
5
2024
Statut:
epublish
Résumé
Spiders are a diverse order of chelicerates that diverged from other arthropods over 500 million years ago. Research on spider embryogenesis, particularly studies using the common house spider Parasteatoda tepidariorum, has made important contributions to understanding the evolution of animal development, including axis formation, segmentation, and patterning. However, we lack knowledge about the cells that build spider embryos, their gene expression profiles and fate. Single-cell transcriptomic analyses have been revolutionary in describing these complex landscapes of cellular genetics in a range of animals. Therefore, we carried out single-cell RNA sequencing of P. tepidariorum embryos at stages 7, 8 and 9, which encompass the establishment and patterning of the body plan, and initial differentiation of many tissues and organs. We identified 20 cell clusters, from 18.5 k cells, which were marked by many developmental toolkit genes, as well as a plethora of genes not previously investigated. We found differences in the cell cycle transcriptional signatures, suggestive of different proliferation dynamics, which related to distinctions between endodermal and some mesodermal clusters, compared with ectodermal clusters. We identified many Hox genes as markers of cell clusters, and Hox gene ohnologs were often present in different clusters. This provided additional evidence of sub- and/or neo-functionalisation of these important developmental genes after the whole genome duplication in an arachnopulmonate ancestor (spiders, scorpions, and related orders). We also examined the spatial expression of marker genes for each cluster to generate a comprehensive cell atlas of these embryonic stages. This revealed new insights into the cellular basis and genetic regulation of head patterning, hematopoiesis, limb development, gut development, and posterior segmentation. This atlas will serve as a platform for future analysis of spider cell specification and fate, and studying the evolution of these processes among animals at cellular resolution.
Identifiants
pubmed: 38730509
doi: 10.1186/s13227-024-00224-4
pii: 10.1186/s13227-024-00224-4
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V014447/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S007849/1
Pays : United Kingdom
Informations de copyright
© 2024. The Author(s).
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