Quantitative analysis of therapeutic peptides by CZE using multiple sample injection in hydrodynamically closed separation system.
capillary zone electrophoresis
hydrodynamically closed separation system
lanreotide
quality control
triptorelin
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
13 May 2024
13 May 2024
Historique:
revised:
03
04
2024
received:
24
02
2024
accepted:
24
04
2024
medline:
13
5
2024
pubmed:
13
5
2024
entrez:
13
5
2024
Statut:
aheadofprint
Résumé
Therapeutic peptides have emerged as an innovative and promising class of therapeutic compounds in modern medicine. Synthetic peptide analogs triptorelin and lanreotide are known for their pronounced clinical versatility and potency. In this study, we present the development and validation of novel methods based on capillary zone electrophoresis performed in hydrodynamically closed system (HCS) and paired with ultraviolet detection and repeated injection sample introduction. To the best of our knowledge, we developed the first capillary electrophoresis-based method for the determination of lanreotide, and concurrently, the first HCS method for the determination of triptorelin. Maximal separation efficiency and signal intensity were achieved using background electrolytes composed of 50 mM formic acid with the addition of 0.05% (v/v) methyl-hydroxyethyl cellulose. The proposed methods exhibit favorable performance characteristics, namely, calibration curve (r
Identifiants
pubmed: 38738565
doi: 10.1002/elps.202400039
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
ID : VEGA 1/0514/22
Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
ID : VEGA 1/0302/24
Informations de copyright
© 2024 Wiley‐VCH GmbH.
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