Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 05 2024
13 05 2024
Historique:
received:
28
11
2023
accepted:
06
05
2024
medline:
14
5
2024
pubmed:
14
5
2024
entrez:
13
5
2024
Statut:
epublish
Résumé
The aim of this study was to evaluate the antimicrobial efficacy of an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods in periodontology. Two types of biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and the other by a complex mixture of saliva microorganisms isolated from a patient with periodontitis. This latter biofilm was characterized via Next Generation Sequencing to determine the main bacterial phyla. The CAP source was applied at a distance of 6 mm for different time points. A statistically significant reduction of both CFU count and XTT was already detected after 60 s of CAP treatment. CLSM analysis supported CAP effectiveness in killing the microorganisms inside the biofilm and in reducing the thickness of the biofilm matrix. Cytotoxicity tests demonstrated the possible use of CAP without important side effects towards human gingival fibroblasts cell line. The current study showed that CAP treatment was able to significantly reduce preformed biofilms developed by both S. mutans and microorganisms isolated by a saliva sample. Further studies should be conducted on biofilms developed by additional saliva donors to support the potential of this innovative strategy to counteract oral pathogens responsible for periodontal diseases.
Identifiants
pubmed: 38740792
doi: 10.1038/s41598-024-61438-z
pii: 10.1038/s41598-024-61438-z
doi:
Substances chimiques
Plasma Gases
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10882Subventions
Organisme : The National Research Foundation (NRF) of Korea
ID : 2021R1A6A1A03038785
Organisme : Ministero Italiano dell'Università e della Ricerca (MIUR)
ID : FAR 2021
Organisme : NextGenerationEU - MUR, Fondo Promozione e Sviluppo, DM 737/2021
ID : CUP number: D75F21003210001
Informations de copyright
© 2024. The Author(s).
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