Incidences of colorectal adenomas and cancers under colonoscopy surveillance suggest an accelerated "Big Bang" pathway to CRC in three of the four Lynch syndromes.

MLH1 MSH2 MSH6 PMS2 Adenoma Colonoscopy Colorectal Lynch syndromes MSI Sojourn time cancer dMMR

Journal

Hereditary cancer in clinical practice
ISSN: 1731-2302
Titre abrégé: Hered Cancer Clin Pract
Pays: Poland
ID NLM: 101231179

Informations de publication

Date de publication:
13 May 2024
Historique:
received: 08 04 2024
accepted: 04 05 2024
medline: 14 5 2024
pubmed: 14 5 2024
entrez: 13 5 2024
Statut: epublish

Résumé

Colorectal cancers (CRCs) in the Lynch syndromes have been assumed to emerge through an accelerated adenoma-carcinoma pathway. In this model adenomas with deficient mismatch repair have an increased probability of acquiring additional cancer driver mutation(s) resulting in more rapid progression to malignancy. If this model was accurate, the success of colonoscopy in preventing CRC would be a function of the intervals between colonoscopies and mean sojourn time of detectable adenomas. Contrary to expectations, colonoscopy did not decrease incidence of CRC in the Lynch syndromes and shorter colonoscopy intervals have not been effective in reducing CRC incidence. The prospective Lynch Syndrome Database (PLSD) was designed to examine these issues in carriers of pathogenic variants of the mis-match repair (path_MMR) genes. We examined the CRC and colorectal adenoma incidences in 3,574 path_MLH1, path_MSH2, path_MSH6 and path_PMS2 carriers subjected to regular colonoscopy with polypectomy, and considered the results based on sojourn times and stochastic probability paradigms. Most of the path_MMR carriers in each genetic group had no adenomas. There was no association between incidences of CRC and the presence of adenomas. There was no CRC observed in path_PMS2 carriers. Colonoscopy prevented CRC in path_PMS2 carriers but not in the others. Our findings are consistent with colonoscopy surveillance blocking the adenoma-carcinoma pathway by removing identified adenomas which might otherwise become CRCs. However, in the other carriers most CRCs likely arised from dMMR cells in the crypts that have an increased mutation rate with increased stochastic chaotic probabilities for mutations. Therefore, this mechanism, that may be associated with no or only a short sojourn time of MSI tumours as adenomas, could explain the findings in our previous and current reports.

Sections du résumé

BACKGROUND BACKGROUND
Colorectal cancers (CRCs) in the Lynch syndromes have been assumed to emerge through an accelerated adenoma-carcinoma pathway. In this model adenomas with deficient mismatch repair have an increased probability of acquiring additional cancer driver mutation(s) resulting in more rapid progression to malignancy. If this model was accurate, the success of colonoscopy in preventing CRC would be a function of the intervals between colonoscopies and mean sojourn time of detectable adenomas. Contrary to expectations, colonoscopy did not decrease incidence of CRC in the Lynch syndromes and shorter colonoscopy intervals have not been effective in reducing CRC incidence. The prospective Lynch Syndrome Database (PLSD) was designed to examine these issues in carriers of pathogenic variants of the mis-match repair (path_MMR) genes.
MATERIALS AND METHODS METHODS
We examined the CRC and colorectal adenoma incidences in 3,574 path_MLH1, path_MSH2, path_MSH6 and path_PMS2 carriers subjected to regular colonoscopy with polypectomy, and considered the results based on sojourn times and stochastic probability paradigms.
RESULTS RESULTS
Most of the path_MMR carriers in each genetic group had no adenomas. There was no association between incidences of CRC and the presence of adenomas. There was no CRC observed in path_PMS2 carriers.
CONCLUSIONS CONCLUSIONS
Colonoscopy prevented CRC in path_PMS2 carriers but not in the others. Our findings are consistent with colonoscopy surveillance blocking the adenoma-carcinoma pathway by removing identified adenomas which might otherwise become CRCs. However, in the other carriers most CRCs likely arised from dMMR cells in the crypts that have an increased mutation rate with increased stochastic chaotic probabilities for mutations. Therefore, this mechanism, that may be associated with no or only a short sojourn time of MSI tumours as adenomas, could explain the findings in our previous and current reports.

Identifiants

pubmed: 38741120
doi: 10.1186/s13053-024-00279-3
pii: 10.1186/s13053-024-00279-3
doi:

Types de publication

Journal Article

Langues

eng

Pagination

6

Subventions

Organisme : The Norwegian Cancer Society
ID : Contract 194751-2017
Organisme : The Norwegian Cancer Society
ID : Contract 194751-2017
Organisme : The Norwegian Cancer Society
ID : Contract 194751-2017
Organisme : Manchester National Institute for Health Research (NIHR) Biomedical Research Centre
ID : IS-BRC-1215-20007
Organisme : Manchester National Institute for Health Research (NIHR) Biomedical Research Centre
ID : IS-BRC-1215-20007

Informations de copyright

© 2024. The Author(s).

Références

Møller P, Seppälä TT, Ahadova A, et al. Dominantly inherited micro-satellite instable cancer - the four Lynch syndromes - an EHTG, PLSD position statement. Hered Cancer Clin Pract. 2023;21(1):19. https://doi.org/10.1186/s13053-023-00263-3 . PMID: 37821984; PMCID: PMC10568908. https://pubmed.ncbi.nlm.nih.gov/37821984/ .
doi: 10.1186/s13053-023-00263-3 pubmed: 37821984 pmcid: 10568908
https://www.omim.org/ Accessed April 2nd 2024.
Chan TA, Wang Z, Dang LH, Vogelstein B, Kinzler KW. Targeted inactivation of CTNNB1 reveals unexpected effects of beta-catenin mutation. Proc Natl Acad Sci U S A. 2002;99(12):8265–70. https://doi.org/10.1073/pnas.082240999 . PMID: 12060769; PMCID: PMC123056. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC123056/ .
doi: 10.1073/pnas.082240999 pubmed: 12060769 pmcid: 123056
Segditsas S, Tomlinson I. Colorectal cancer and genetic alterations in the wnt pathway. Oncogene. 2006;25:7531–7. https://doi.org/10.1038/sj.onc.1210059 .
doi: 10.1038/sj.onc.1210059 pubmed: 17143297
Jass JR, Stewart SM. Evolution of hereditary non-polyposis colorectal cancer. Gut. 1992;33(6):783–6. https://doi.org/10.1136/gut.33.6.783 . PMID: 1624160; PMCID: PMC1379336. https://pubmed.ncbi.nlm.nih.gov/1624160/ .
doi: 10.1136/gut.33.6.783 pubmed: 1624160 pmcid: 1379336
Ahadova A, Gallon R, Gebert J, et al. Three molecular pathways model colorectal carcinogenesis in Lynch syndrome. Int J Cancer. 2018;143(1):139–50. https://doi.org/10.1002/ijc.31300 . Epub 2018 Feb 23. PMID:. https://pubmed.ncbi.nlm.nih.gov/29424427/ .
doi: 10.1002/ijc.31300 pubmed: 29424427
Hans FA, Vasen I, Blanco K, Aktan-Collan, et al. Revised guidelines for the clinical management of Lynch syndrome (HNPCC): recommendations by a group of European experts. Gut. 2013;62:812–23. https://doi.org/10.1136/gutjnl-2012-304356 . https://gut.bmj.com/content/gutjnl/62/6/812.full.pdf .
doi: 10.1136/gutjnl-2012-304356
Engel C, Vasen HF, Seppälä T et al. No Difference in Colorectal Cancer Incidence or Stage at Detection by Colonoscopy Among 3 Countries With Different Lynch Syndrome Surveillance Policies. Gastroenterology. 2018;155(5):1400–1409.e2. https://doi.org/10.1053/j.gastro.2018.07.030 . Epub 2018 Jul 29. PMID: 30063918. https://pubmed.ncbi.nlm.nih.gov/30063918/ .
Seppälä TT, Ahadova A, Dominguez-Valentin M, et al. Lack of association between screening interval and cancer stage in Lynch syndrome may be accounted for by over-diagnosis; a prospective Lynch syndrome database report. Hered Cancer Clin Pract. 2019;17:8. https://doi.org/10.1186/s13053-019-0106-8 . PMID: 30858900; PMCID: PMC6394091. https://pubmed.ncbi.nlm.nih.gov/30858900/ .
doi: 10.1186/s13053-019-0106-8 pubmed: 30858900 pmcid: 6394091
Møller P, Seppälä T, Dowty JG, et al. Colorectal cancer incidences in Lynch syndrome: a comparison of results from the prospective lynch syndrome database and the international mismatch repair consortium. Hered Cancer Clin Pract. 2022;20(1):36. https://doi.org/10.1186/s13053-022-00241-1 . PMID: 36182917; PMCID: PMC9526951. https://pubmed.ncbi.nlm.nih.gov/36182917/ .
doi: 10.1186/s13053-022-00241-1 pubmed: 36182917 pmcid: 9526951
Bonadona V, Bonaïti B, Olschwang S et al. Cancer risks associated with germline mutations in MLH1, MSH2, and MSH6 genes in Lynch syndrome. JAMA. 2011;305(22):2304-10. https://doi.org/10.1001/jama.2011.743 . PMID: 21642682. https://pubmed.ncbi.nlm.nih.gov/21642682/ .
Hampel H, Stephens JA, Pukkala E, Sankila R, Aaltonen LA, Mecklin JP, de la Chapelle A. Cancer risk in hereditary nonpolyposis colorectal cancer syndrome: later age of onset. Gastroenterology. 2005;129(2):415 – 21. https://doi.org/10.1016/j.gastro.2005.05.011 . PMID: 16083698. https://pubmed.ncbi.nlm.nih.gov/16083698/ .
Baglietto L, Lindor NM, Dowty JG, et al. Risks of Lynch syndrome cancers for MSH6 mutation carriers. J Natl Cancer Inst. 2010;102(3):193–201. https://doi.org/10.1093/jnci/djp473 . Epub 2009 Dec 22. PMID: 20028993; PMCID: PMC2815724. https://pubmed.ncbi.nlm.nih.gov/20028993/ .
doi: 10.1093/jnci/djp473 pubmed: 20028993 pmcid: 2815724
Ten Broeke SW, van der Klift HM, Tops CMJ et al. Cancer Risks for PMS2-Associated Lynch Syndrome. J Clin Oncol. 2018;36(29):2961–2968. doi: 10.1200/JCO.2018.78.4777. Epub 2018 Aug 30. Erratum in: J Clin Oncol. 2019;37(9):761. PMID: 30161022; PMCID: PMC6349460. https://pubmed.ncbi.nlm.nih.gov/30161022/ .
Kloor M, Huth C, Voigt AY, Benner A, Schirmacher P, von Knebel Doeberitz M, Bläker H. Prevalence of mismatch repair-deficient crypt foci in Lynch syndrome: a pathological study. Lancet Oncol. 2012;13(6):598–606. doi: 10.1016/S1470-2045(12)70109-2. Epub 2012 May 1. PMID: 22552011. https://pubmed.ncbi.nlm.nih.gov/22552011/ .
Pai RK, Dudley B, Karloski E, et al. DNA mismatch repair protein deficient non-neoplastic colonic crypts: a novel indicator of Lynch syndrome. Mod Pathol. 2018;31(10):1608–18. https://doi.org/10.1038/s41379-018-0079-6 . Epub 2018 Jun 8. PMID: 29884888; PMCID: PMC6396289. https://pubmed.ncbi.nlm.nih.gov/29884888/ .
doi: 10.1038/s41379-018-0079-6 pubmed: 29884888 pmcid: 6396289
Brand RE, Dudley B, Karloski E, Das R, Fuhrer K, Pai RK, Pai RK. Detection of DNA mismatch repair deficient crypts in random colonoscopic biopsies identifies Lynch syndrome patients. Fam Cancer. 2020;19(2):169–175. https://doi.org/10.1007/s10689-020-00161-w . PMID: 31997046. https://pubmed.ncbi.nlm.nih.gov/31997046/ .
Cercek A, Lumish M, Sinopoli J, et al. PD-1 blockade in Mismatch Repair-Deficient, locally advanced rectal Cancer. N Engl J Med. 2022;386(25):2363–76. https://doi.org/10.1056/NEJMoa2201445 . Epub 2022 Jun 5. PMID: 35660797; PMCID: PMC9492301. https://pubmed.ncbi.nlm.nih.gov/35660797/ .
doi: 10.1056/NEJMoa2201445 pubmed: 35660797 pmcid: 9492301
Ahadova A, Seppälä TT, Engel C et al. The unnatural history of colorectal cancer in Lynch syndrome: Lessons from colonoscopy surveillance. Int J Cancer. 2021;148(4):800–811. doi: 10.1002/ijc.33224. Epub 2020 Aug 3. PMID: 32683684. https://pubmed.ncbi.nlm.nih.gov/32683684/ .
Haupt S, Zeilmann A, Ahadova A, Bläker H, von Knebel Doeberitz M, Kloor M, Heuveline V. Mathematical modeling of multiple pathways in colorectal carcinogenesis using dynamical systems with Kronecker structure. PLoS Comput Biol. 2021;17(5):e1008970. https://doi.org/10.1371/journal.pcbi.1008970 . PMID: 34003820; PMCID: PMC8162698. https://pubmed.ncbi.nlm.nih.gov/34003820/ .
Møller P, Seppälä T, Bernstein I, et al. Cancer incidence and survival in Lynch syndrome patients receiving colonoscopic and gynaecological surveillance: first report from the prospective Lynch syndrome database. Gut. 2017;66(3):464–72. Epub 2015 Dec 9. PMID: 26657901; PMCID: PMC5534760. https://pubmed.ncbi.nlm.nih.gov/26657901/ . doi: 10.1136/gutjnl-2015-309675.
doi: 10.1136/gutjnl-2015-309675 pubmed: 26657901
Møller P. The prospective Lynch Syndrome Database: background, design, main results and complete MySQL code. Hered Cancer Clin Pract. 2022;20(1):37. https://doi.org/10.1186/s13053-022-00243-z . PMID: 36411472; PMCID: PMC9677689. https://pubmed.ncbi.nlm.nih.gov/36411472/ .
doi: 10.1186/s13053-022-00243-z pubmed: 36411472 pmcid: 9677689
Møller P. The prospective Lynch Syndrome database reports enable evidence-based personal precision health care. Hered Cancer Clin Pract. 2020;18:6. https://doi.org/10.1186/s13053-020-0138-0 . PMID: 32190163; PMCID: PMC7073013. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073013/ .
doi: 10.1186/s13053-020-0138-0 pubmed: 32190163 pmcid: 7073013
Dominguez-Valentin M, Haupt S, Seppälä TT et al. Mortality by age, gene and gender in carriers of pathogenic mismatch repair gene variants receiving surveillance for early cancer diagnosis and treatment: a report from the prospective Lynch syndrome database. EClinicalMedicine. 2023;58:101909. https://doi.org/10.1016/j.eclinm.2023.101909 . PMID: 37181409; PMCID: PMC10166779. https://pubmed.ncbi.nlm.nih.gov/37181409/ .
https:// en.wikipedia.org/wiki/Mean_sojourn_time Accessed April 2nd 2024.
Prevost TC, Launoy G, Duffy SW, Chen HH. Estimating sensitivity and sojourn time in screening for colorectal cancer: a comparison of statistical approaches. Am J Epidemiol. 1998;148(6):609 – 19. https://doi.org/10.1093/oxfordjournals.aje.a009687 . PMID: 9753016. https://pubmed.ncbi.nlm.nih.gov/9753016/ .
https:// en.wikipedia.org/wiki/Poisson_distribution Accessed April 2nd 2024.
https:// www.britannica.com/science/stochastic-process Accessed April 2nd 2024.
https:// en.wikipedia.org/wiki/Introduction_to_quantum_mechanics Accessed April 2nd 2024.
Annelie Liljegren G, Barker F, Elliott et al. Polyps in Mismatch Repair Mutation Carriers Among CAPP2 Participants: Report by the Colorectal Adenoma/Carcinoma Prevention Programme 2. JCO. 2008; 26; 20; 3434-9 https://doi.org/10.1200/JCO.2007.13.2795 .
Ahadova A, von Knebel Doeberitz M, Bläker H et al. CTNNB1-mutant colorectal carcinomas with immediate invasive growth: a model of interval cancers in Lynch syndrome. Familial Cancer 15, 579–586 (2016). https://doi.org/10.1007/s10689 -016-9899-z https://link.springer.com/article/10.1007/s10689-016-9899-z.
Ahadova A, Stenzinger A, Seppälä T, Hüneburg R, Kloor M, Bläker H. Lynpath Investigators. A Two-in-One Hit Model of Shortcut Carcinogenesis in MLH1 Lynch Syndrome Carriers. Gastroenterology. 2023;165(1):267–270.e4. https://doi.org/10.1053/j.gastro.2023.03.007 . Epub 2023 Mar 11. PMID: 36907525. https://pubmed.ncbi.nlm.nih.gov/36907525/ .
Helderman NC, Van Der Werf-‘t Lam AS, MSH6 TUMOR GROUP, Morreau H, Boot A, Van Wezel T, Nielsen M. Gastroenterology. 2023;165(1):271–e2742. https://doi.org/10.1053/j.gastro.2023.03.198 . Epub 2023 Mar 15. PMID: 36931573. https://pubmed.ncbi.nlm.nih.gov/36931573/ . Molecular Profile of MSH6-Associated Colorectal Carcinomas Shows Distinct Features From Other Lynch Syndrome-Associated Colorectal Carcinomas.
Ten Broeke SW, van Bavel TC, Jansen AML et al. Molecular Background of Colorectal Tumors From Patients With Lynch Syndrome Associated With Germline Variants in PMS2. Gastroenterology. 2018;155(3):844–851. https://doi.org/10.1053/j.gastro.2018.05.020 . Epub 2018 Jul 29. PMID: 29758216. https://pubmed.ncbi.nlm.nih.gov/29758216/ .
https:// www.google.com/search?client=firefox-b-d&q=heisenberg+uncertainty+principle Accessed April 2nd 2024.
Staffa L, Echterdiek F, Nelius N, et al. Mismatch repair-deficient crypt foci in Lynch syndrome–molecular alterations and association with clinical parameters. PLoS ONE. 2015;10(3):e0121980. https://doi.org/10.1371/journal.pone.0121980 . PMID: 25816162; PMCID: PMC4376900. https://pubmed.ncbi.nlm.nih.gov/25816162/ .
doi: 10.1371/journal.pone.0121980 pubmed: 25816162 pmcid: 4376900
Sottoriva A, Kang H, Ma Z, et al. A Big Bang model of human colorectal tumor growth. Nat Genet. 2015;47:209–16. https://doi.org/10.1038/ng.3214 . https://www.nature.com/articles/ng.3214 .
doi: 10.1038/ng.3214 pubmed: 25665006 pmcid: 4575589
Chien J, Neums L, Powell AFLA, Torres M, Kalli KR, Multinu F, Shridhar V, Mariani A. Genetic Evidence for Early Peritoneal Spreading in Pelvic High-Grade Serous Cancer. Front Oncol. 2018;8:58. https://doi.org/10.3389/fonc.2018.00058 . PMID: 29594039; PMCID: PMC5858520. https://pubmed.ncbi.nlm.nih.gov/29594039/ .
Dominguez-Valentin M, Seppälä TT, Sampson JR, et al. Survival by colon cancer stage and screening interval in Lynch syndrome: a prospective Lynch syndrome database report. Hered Cancer Clin Pract. 2019;17:28. https://doi.org/10.1186/s13053-019-0127-3 . PMID: 31636762; PMCID: PMC6792227. https://pubmed.ncbi.nlm.nih.gov/31636762/ .
doi: 10.1186/s13053-019-0127-3 pubmed: 31636762 pmcid: 6792227
Mangas-Sanjuan C, de-Castro L, Cubiella J et al. Role of Artificial Intelligence in Colonoscopy Detection of Advanced Neoplasias: A Randomized Trial. Ann Intern Med. 2023;176(9):1145–1152. doi: 10.7326/M22-2619. Epub 2023 Aug 29. PMID: 37639723. https://pubmed.ncbi.nlm.nih.gov/37639723/ .
Hüneburg R, Bucksch K, Schmeißer F, et al. Real-time use of artificial intelligence (CADEYE) in colorectal cancer surveillance of patients with Lynch syndrome-A randomized controlled pilot trial (CADLY). United Eur Gastroenterol J. 2023;11(1):60–8. https://doi.org/10.1002/ueg2.12354 . Epub 2022 Dec 26. PMID: 36571259; PMCID: PMC9892476. https://pubmed.ncbi.nlm.nih.gov/36571259/ .
doi: 10.1002/ueg2.12354
Sánchez A, Roos VH, Navarro M et al. Quality of Colonoscopy Is Associated With Adenoma Detection and Postcolonoscopy Colorectal Cancer Prevention in Lynch Syndrome. Clin Gastroenterol Hepatol. 2022;20(3):611–621.e9. https://doi.org/10.1016/j.cgh.2020.11.002 . Epub 2020 Nov 3. PMID: 33157315. https://pubmed.ncbi.nlm.nih.gov/33157315/ .
Sleiman J, Farha N, Beard J et al. Incidence and prevalence of advanced colorectal neoplasia in Lynch syndrome. Gastrointest Endosc. 2023;98(3):412–419.e8. https://doi.org/10.1016/j.gie.2023.04.001 . Epub 2023 Apr 7. PMID: 37031913. https://pubmed.ncbi.nlm.nih.gov/37031913/ .
Aronson M, Gryfe R, Choi YH, et al. Evaluating colonoscopy screening intervals in patients with Lynch syndrome from a large Canadian registry. J Natl Cancer Inst. 2023;115(7):778–87. https://doi.org/10.1093/jnci/djad058 . PMID: 36964717; PMCID: PMC10323893. https://pubmed.ncbi.nlm.nih.gov/36964717/ .
doi: 10.1093/jnci/djad058 pubmed: 36964717 pmcid: 10323893
Del Carmen G, Reyes-Uribe L, Goyco D, et al. Colorectal surveillance outcomes from an institutional longitudinal cohort of lynch syndrome carriers. Front Oncol. 2023;13:1146825. https://doi.org/10.3389/fonc.2023.1146825 . PMID: 37168379; PMCID: PMC10164917. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164917/ .
doi: 10.3389/fonc.2023.1146825 pubmed: 37168379 pmcid: 10164917
Henry T, Lync JF, Lynch R, Fitzgibbons. Jr. Role of prophylactic colectomy in Lynch Syndrome. Clin Colorectal Cancer. 2003,3;2; 99-101August 2003; 99 https://www.sciencedirect.com/science/article/pii/S1533002811700743?via%3Dihub .
Cavestro GM, Mannucci A, Balaguer F, et al. Delphi Initiative for Early-Onset Colorectal Cancer (DIRECt) International Management guidelines. Clin Gastroenterol Hepatol. 2023;21(3):581–e60333. https://doi.org/10.1016/j.cgh.2022.12.006 . Epub 2022 Dec 20. PMID:. https://pubmed.ncbi.nlm.nih.gov/36549470/ .
doi: 10.1016/j.cgh.2022.12.006 pubmed: 36549470
Seppälä TT, Latchford A, Negoi I et al. European guidelines from the EHTG and ESCP for Lynch syndrome: an updated third edition of the Mallorca guidelines based on gene and gender. Br J Surg. 2021;108(5):484–498. https://doi.org/10.1002/bjs.11902 . PMID: 34043773; PMCID: PMC10364896. https://pubmed.ncbi.nlm.nih.gov/34043773/ .

Auteurs

Pål Møller (P)

Department of Tumour Biology, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo, 0379, Norway. moller.pal@gmail.com.

Saskia Haupt (S)

Engineering Mathematics and Computing Lab (EMCL), Interdisciplinary Center for Scientific Computing (IWR), Heidelberg University, Heidelberg, Germany.
Data Mining and Uncertainty Quantification (DMQ), Heidelberg Institute for Theoretical Studies (HITS), Heidelberg, Germany.

Aysel Ahadova (A)

Department of Applied Tumour Biology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Clinical Cooperation Unit Applied Tumour Biology, German Cancer Research Centre (DKFZ), Heidelberg, Germany.

Matthias Kloor (M)

Department of Applied Tumour Biology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Clinical Cooperation Unit Applied Tumour Biology, German Cancer Research Centre (DKFZ), Heidelberg, Germany.

Julian R Sampson (JR)

Institute of Medical Genetics, Division of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, CF14 4XN, UK.

Lone Sunde (L)

Department of Clinical Genetics, Aalborg University Hospital, Aalborg, 9000, Denmark.
Department of Biomedicine, Aarhus University, Aarhus, DK-8000, Denmark.
Clinical Cancer Research Center, Aalborg University Hospital, Aalborg, Denmark.

Toni Seppälä (T)

Faculty of Medicine and Health Technology, Tays Cancer Center, Tampere University, Tampere University Hospital, Tampere, Finland.
Department of Gastrointestinal Surgery, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland.
Applied Tumour Genomics, Research Program Unit, University of Helsinki, Helsinki, Finland.

John Burn (J)

Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.

Inge Bernstein (I)

Dept. of Quality and Coherence, Aalborg University Hospital, Aalborg, 9000, Denmark.
Department of Clinical Medicine, Aalborg University Hospital, Aalborg University, Aalborg, 9100, Denmark.

Gabriel Capella (G)

Hereditary Cancer Program, Institut Català d'Oncologia-IDIBELL, L; Hospitalet de Llobregat, Barcelona, 08908, Spain.

D Gareth Evans (DG)

Manchester Centre for Genomic Medicine, Division of Evolution, Infection and Genomic Sciences, University of Manchester, Manchester University NHS Foundation Trust, Manchester, M13 9WL, UK.

Annika Lindblom (A)

Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, 171 76, Sweden.
Dept Clinical Genetics, Karolinska University Hospital, Solna, Sweden.

Ingrid Winship (I)

Genomic Medicine, The Royal Melbourne Hospital, Melbourne, Australia.
Department of Medicine, University of Melbourne, Melbourne, Australia.

Finlay Macrae (F)

Department of Medicine, University of Melbourne, Melbourne, Australia.

Lior Katz (L)

Department of Gastroenterology, Medical Center, Faculty of Medicine, Hebrew University of Jerusalem, Hadassah, Israel.

Ido Laish (I)

Gastroenerolgy institute, Sheba medical center and Faculty of medicine Tel Aviv university, Tel Aviv, Israel.

Elez Vainer (E)

Department of Gastroenterology, Medical Center, Faculty of Medicine, Hebrew University of Jerusalem, Hadassah, Israel.

Kevin Monahan (K)

Lynch Syndrome & Family Cancer Clinic, Centre for Familial Intestinal Caner, St Mark's Hospital, London, UK.

Elizabeth Half (E)

Gastrointestinal Cancer Prevention Unit, Gastroenterology Department, Rambam Health Care Campus, Haifa, Israel.

Karoline Horisberger (K)

Department of Surgery, Universitätsmedizin Mainz, Mainz, Germany.

Leandro Apolinário da Silva (LA)

Hospital Universitário Oswaldo Cruz, Universidade de Pernambuco, Recife, Brazil & SEQUIPE, Recife, Brazil.

Vincent Heuveline (V)

Engineering Mathematics and Computing Lab (EMCL), Interdisciplinary Center for Scientific Computing (IWR), Heidelberg University, Heidelberg, Germany.
Data Mining and Uncertainty Quantification (DMQ), Heidelberg Institute for Theoretical Studies (HITS), Heidelberg, Germany.

Christina Therkildsen (C)

Gastro Unit, The Danish HNPCC Register, Copenhagen University Hospital - Amager and Hvidovre, Copenhagen, Denmark.

Charlotte Lautrup (C)

Department of Clinical Genetics, Aarhus University Hospital, DK 8000, Aarhus, Denmark.

Louise L Klarskov (LL)

Dept of Pathology, Copenhagen University Hospital - Herlev and Gentofte, Herlev, Denmark.
Dept of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.

Giulia Martina Cavestro (GM)

Gastroenterology and Gastrointestinal Endoscopy Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Vita-Salute San Raffaele University, 20132, Milan, Italy.

Gabriela Möslein (G)

Surgical Center for Hereditary Tumors, University Düsseldorf, Ev. Bethesda Khs, Duisburg, Germany.

Eivind Hovig (E)

Department of Tumour Biology, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo, 0379, Norway.
Centre for bioinformatics, Department of Informatics, University of Oslo, Oslo, Norway.

Mev Dominguez-Valentin (M)

Department of Tumour Biology, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo, 0379, Norway.

Classifications MeSH