Introducing the glycyrrhizic acid and glabridin rich genotypes from the cultivated Iranian licorice (Glycyrrhiza glabra L.) populations to exploit in production systems.
Fabaceae
Flavonoid
Liquorice
Phytochemical analysis
Specialized metabolites
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 05 2024
14 05 2024
Historique:
received:
28
11
2023
accepted:
08
05
2024
medline:
15
5
2024
pubmed:
15
5
2024
entrez:
14
5
2024
Statut:
epublish
Résumé
Currently, the stable, uniform, and highly efficient production of raw materials for pharmaceutical companies has received special attention. To meet these criteria and reduce harvesting pressure on the natural habitats of licorice (Glycyrrhiza glabra L.), cultivation of this valuable plant is inevitable. In the present study, to introduce the glycyrrhizic acid (GA)- and glabridin-rich genotypes from cultivated Iranian licorice, forty genotypes from eight high-potential wild populations were cultivated and evaluated under the same environmental conditions. The GA content varied from 5.00 ± 0.04 mg/g DW (TF2 genotype) to 23.13 ± 0.02 mg/g DW (I5 genotype). The highest and lowest glabridin content were found in the K2 (0.72 ± 0.021 mg/g DW) and M5 (0.02 ± 0.002 mg/g DW) genotypes, respectively. The rutin content in the leaves of the studied genotypes varied from 1.27 ± 0.02 mg/g DW in E4 to 3.24 ± 0.02 mg/g DW in BO5 genotypes. The genotypes from the Ilam population were characterized by higher vegetative growth and yield traits in the aerial parts and roots. The average root dry yield was 2.44 tons per hectare (t/ha) among the studied genotypes and a genotype from Ilam (I5) yielded the maximum value (3.08 ± 0.034 t/ha). The highest coefficient of variation among the genotypes was observed for leaf width (CV = 34.9%). The GA and glabridin-rich genotypes introduced in this study can be used in the future breeding programs to release new bred licorice cultivars.
Identifiants
pubmed: 38744977
doi: 10.1038/s41598-024-61711-1
pii: 10.1038/s41598-024-61711-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11034Informations de copyright
© 2024. The Author(s).
Références
Öztürk, M., Altay, V., Hakeem, K. R. & Akçiçek, E. Liquorice: from Botany to Phytochemistry (Springer, 2018).
Ding, Y., Brand, E., Wang, W. & Zhao, Z. Licorice: Resources, applications in ancient and modern times. J. Ethnopharmacol. 298, 115594 (2022).
pubmed: 35934191
doi: 10.1016/j.jep.2022.115594
Pastorino, G., Cornara, L., Soares, S., Rodrigues, F. & Oliveira, M. B. P. Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytother. Res. 32, 2323–2339 (2018).
pubmed: 30117204
pmcid: 7167772
doi: 10.1002/ptr.6178
Mamedov, N. A. & Egamberdieva, D. Phytochemical constituents and pharmacological effects of licorice: A review. Plant Hum. Health 3, 1–21 (2019).
Ashfaq, U. A., Masoud, M. S., Nawaz, Z. & Riazuddin, S. Glycyrrhizin as antiviral agent against hepatitis C virus. J. Transl. Med. 9(1), 1–7 (2011).
doi: 10.1186/1479-5876-9-112
Su, X. et al. Glycyrrhizic acid: A promising carrier material for anticancer therapy. Biomed. Pharmacother. 95, 670–678 (2017).
pubmed: 28886526
doi: 10.1016/j.biopha.2017.08.123
Huo, X., Meng, X., Zhang, J. & Zhao, Y. Hepatoprotective effect of different combinations of 18a-and 18b-glycyrrhizic acid against CCl4-induced liver injury in rats. Biomed. Pharmacother. 122, 109354 (2020).
pubmed: 31918260
doi: 10.1016/j.biopha.2019.109354
Zhao, Z. Y. et al. Glycyrrhizic acid nanoparticles as antiviral and anti-inflammatory agents for COVID-19 treatment. ACS Appl. Mater. Interfaces 13, 20995–21006 (2021).
pubmed: 33930273
doi: 10.1021/acsami.1c02755
Riccioni, G. et al. Novel phytonutrient contributors to antioxidant protection against cardiovascular disease. Nutrition 28, 605–610 (2012).
pubmed: 22480801
doi: 10.1016/j.nut.2011.11.028
Kang, M. R. et al. Cardiovascular protective effect of glabridin: Implications in LDL oxidation and inflammation. Int. Immunopharmacol. 29, 914–918 (2015).
pubmed: 26526087
doi: 10.1016/j.intimp.2015.10.020
Su Wei Poh, M., Voon Chen Yong, P., Viseswaran, N. & Chia, Y. Y. Estrogenicity of glabridin in Ishikawa cells. PLoS One 10, e0121382 (2015).
pubmed: 25816349
pmcid: 4376725
doi: 10.1371/journal.pone.0121382
Akbar, S. Glycyrrhiza glabra L. (Fabaceae/Leguminosae). In Handbook of 200 Medicinal Plants 963–980 (Springer, 2020).
doi: 10.1007/978-3-030-16807-0_103
Li, C. X., Li, T. H., Zhu, M., Lai, J. & Wu, Z. P. Pharmacological properties of glabridin (a flavonoid extracted from licorice): A comprehensive review. J. Funct. Foods 85, 104638 (2021).
doi: 10.1016/j.jff.2021.104638
Simmler, C., Pauli, G. F. & Chen, S. N. Phytochemistry and biological properties of glabridin. Fitoterapia 90, 160–184 (2013).
pubmed: 23850540
doi: 10.1016/j.fitote.2013.07.003
Canter, P. H., Thomas, H. & Ernst, E. Bringing medicinal plants into cultivation: Opportunities and challenges for biotechnology. Trends Biotechnol. 23, 180–185 (2005).
pubmed: 15780709
doi: 10.1016/j.tibtech.2005.02.002
Nemeth, E., Bernath, J. & Hethelyi, E. Chemotypes and their stability in Achillea crithmifolia populations. J. Essent. Oil Res. 12, 53–58 (2000).
doi: 10.1080/10412905.2000.9712041
Ramawat, K. G. & Arora, J. Medicinal plants domestication, cultivation, improvement, and alternative technologies for the production of high value therapeutics: An overview. In Medicinal Plants: Domestication, Biotechnology and Regional Importance, 1–29 (2021).
Chen, Y. G. et al. Impacts of heavy metals and medicinal crops on ecological systems, environmental pollution, cultivation, and production processes in China. Ecotoxicol. Environ. Saf. 219, 112336 (2021).
pubmed: 34044310
doi: 10.1016/j.ecoenv.2021.112336
Selseleh, M. et al. Metabolic diversity and genetic association between wild populations of Verbascum songaricum (Scrophulariaceae). Ind. Crops Prod. 137, 112–125 (2019).
doi: 10.1016/j.indcrop.2019.03.069
Wang, W., Xu, J., Fang, H., Li, Z. & Li, M. Advances and challenges in medicinal plant breeding. Plant Sci. 298, 110573 (2020).
pubmed: 32771174
doi: 10.1016/j.plantsci.2020.110573
Shahriari, Z., Heidari, B. & Dadkhodaie, A. Dissection of genotype× environment interactions for mucilage and seed yield in Plantago species: Application of AMMI and GGE biplot analyses. PLoS One 13, e0196095 (2018).
pubmed: 29715274
pmcid: 5929508
doi: 10.1371/journal.pone.0196095
He, M. et al. Accurate recognition and feature qualify for flavonoid extracts from Liang-wai Gan Cao by liquid chromatography-high resolution-mass spectrometry and computational MS/MS fragmentation. J. Pharm. Biomed. Anal. 146, 37–47 (2017).
pubmed: 28850862
doi: 10.1016/j.jpba.2017.07.065
Wang, C. et al. Distribution patterns for metabolites in medicinal parts of wild and cultivated licorice. J. Pharm. Biomed. Anal. 161, 464–473 (2018).
pubmed: 30218948
doi: 10.1016/j.jpba.2018.09.004
Chen, K. Z., Song, H. & Ruyu, C. Licorice Industry in China: Implications for Licorice Producers in Uzbekistan (Intl Food Policy Res Inst, 2014).
Han, Y., Pang, X., Zhang, X., Han, R. & Liang, Z. Resource sustainability and challenges: Status and competitiveness of international trade in licorice extracts under the Belt and Road Initiative. Glob. Ecol. Conserv. 34, e02014 (2022).
Khaitov, B. et al. Perspectives of licorice production in harsh environments of the Aral Sea regions. Int. J. Environ. Res. Pub Health 19, 11770 (2022).
doi: 10.3390/ijerph191811770
Esmaeili, H., Karami, A., Hadian, J., Saharkhiz, M. J. & Ebrahimi, S. N. Variation in the phytochemical contents and antioxidant activity of Glycyrrhiza glabra populations collected in Iran. Ind. Crops Prod. 137, 248–259 (2019).
doi: 10.1016/j.indcrop.2019.05.034
Esmaeili, H., Karami, A., Hadian, J., Ebrahimi, S. N. & Otto, L. G. Genetic structure and variation in Iranian licorice (Glycyrrhiza glabra L.) populations based on morphological, phytochemical and simple sequence repeats markers. Ind. Crops Prod. 145, 112140 (2020).
doi: 10.1016/j.indcrop.2020.112140
Lister, E. & Wilson, P. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity (personal Communication) 235–239 (Crop Research Institute, 2001).
Zhishen, J., Mengcheng, T. & Jianming, W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555–559 (1999).
doi: 10.1016/S0308-8146(98)00102-2
Blois, M. S. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199–1200 (1958).
doi: 10.1038/1811199a0
Hiyama, H., Ozawa, A., Kumazawa, H. & Takeda, O. Stabilization of ephedrine alkaloid content in Ephedra sinica by selective breeding and stolon propagation. Biol. Pharm. Bull. 40, 43–48 (2017).
pubmed: 28049947
doi: 10.1248/bpb.b16-00531
Ahn, I. O., Lee, S. S., Lee, J. H., Lee, M. J. & Jo, B. G. Comparison of ginsenoside contents and pattern similarity between root parts of new cultivars in Panax ginseng CA Meyer. J. Ginseng Res. 32, 15–18 (2008).
doi: 10.5142/JGR.2008.32.1.015
Sharifi, T. M., Kazemi, A. & Shabani, L. Phenetic relationships among natural population accessions of Glycyrrhiza glabra L. (Fabaceae) in central Zagros region of Iran, based on quantitative morphology, flavonoids and glycyrrhizin contents data. Taxon. Biosyst. 13, 59–72 (2013).
Ozaki, K., Shibano, M., Kusano, G. & Watanabe, H. Aim for production of Glycyrrhizae Radix in Japan (2). Selection of pharmaceutically fine strains from Glycyrrhiza uralensis Fisher. Shoyakugaku Zasshi 64, 76–82 (2010).
Inui, T. et al. Development of selection method for Glycyrrhiza uralensis superior clones with high-glycyrrhizic acid contents using DNA sequence polymorphisms in glycyrrhizic acid biosynthetic genes. Plant Biotechnol. 38, 127–135 (2021).
doi: 10.5511/plantbiotechnology.21.0112a
Wang, H. et al. Identification wild and cultivated licorice by multidimensional analysis. Food Chem. 339, 128111 (2021).
pubmed: 33152888
doi: 10.1016/j.foodchem.2020.128111
Hayashi, H., Hiraoka, N., Ikeshiro, Y., Yamamoto, H. & Yoshikawa, T. Seasonal variation of glycyrrhizin and isoliquiritigenin glycosides in the root of Glycyrrhiza glabra L. Biol. Pharm. Bull. 21, 987–989 (1998).
pubmed: 9781853
doi: 10.1248/bpb.21.987
Li, X., Guo, R., Zhang, X. & Li, X. Extraction of glabridin using imidazolium-based ionic liquids. Sep. Purif. Technol. 88, 146–150 (2012).
doi: 10.1016/j.seppur.2011.12.018
Fenwick, G. R., Lutomski, J. & Nieman, C. Liquorice, Glycyrrhiza glabra L.—Composition, uses and analysis. Food Chem. 38, 119–143 (1990).
doi: 10.1016/0308-8146(90)90159-2
Kovalenko, P. G., Antonjuk, V. P. & Maliuta, S. S. Secondary metabolites synthesis in transformed cells of Glycyrrhiza glabra L. and Potentilla alba L. as producents of radioprotective compounds. Ukr. Bioorg. Acta. 1, 13–22 (2004).
Zhang, J. T., Xu, B. & Li, M. Relationships between the bioactive compound content and environmental variables in Glycyrrhiza uralensis populations in different habitats of North China. Phyton (Buenos Aires) 80, 161–166 (2011).
Statti, G. A. et al. Variability in the content of active constituents and biological activity of Glycyrrhiza glabra. Fitoterapia 75, 371–374 (2004).
pubmed: 15158998
doi: 10.1016/j.fitote.2003.12.022
Hayashi, H. et al. Field survey of Glycyrrhiza plants in Central Asia (3). Chemical characterization of G. glabra collected in Uzbekistan. Chem. Pharm. Bull. 51, 1338–1340 (2003).
doi: 10.1248/cpb.51.1338
Dražić, S. et al. Effect of environment of the rutin content in leaves of Fagopyrum esculentum Moench. Plant Soil Environ. 62, 261–265 (2016).
doi: 10.17221/233/2016-PSE
Asan-Ozusaglam, M. & Karakoca, K. Evaluation of biological activity and antioxidant capacity of Turkish licorice root extracts. Rom. Biotechnol. Lett. 19, 8994–9005 (2014).
Sohail, M., Rakha, A., Butt, M. S. & Asghar, M. Investigating the antioxidant potential of licorice extracts obtained through different extraction modes. J. Food Biochem. 42, e12466 (2018).
doi: 10.1111/jfbc.12466
Vlaisavljević, S. et al. Chemical composition, antioxidant and anticancer activity of licorice from Fruska Gora locality. Ind. Crops Prod. 112, 217–224 (2018).
doi: 10.1016/j.indcrop.2017.11.050
Dong, Y. et al. Bioactive profiles, antioxidant activities, nitrite scavenging capacities and protective effects on H
pubmed: 24983860
pmcid: 6270937
doi: 10.3390/molecules19079101
Djeridane, A. et al. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 97, 654–660 (2006).
doi: 10.1016/j.foodchem.2005.04.028
Yordi, E. G., Pérez, E. M., Matos, M. J. & Villares, E. U. Antioxidant and pro-oxidant effects of polyphenolic compounds and structure–activity relationship evidence. Nutr. Well-being Health 2, 23–48 (2012).
Li, M. et al. Antioxidant capacity connection with phenolic and flavonoid content in Chinese medicinal herbs. Rec. Nat. Prod. 12, 239–250 (2018).
doi: 10.25135/rnp.24.17.08.138
Muflihah, Y. M., Gollavelli, G. & Ling, Y. C. Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs. Antioxidants 10, 1530 (2021).
pubmed: 34679665
pmcid: 8533117
doi: 10.3390/antiox10101530
Haraguchi, H. et al. Protection of mitochondrial functions against oxidative stresses by isoflavans from Glycyrrhiza glabra. J. Pharm. Pharmacol. 52, 219–223 (2000).
pubmed: 10714953
doi: 10.1211/0022357001773724
Carmeli, E. & Fogelman, Y. Antioxidant effect of polyphenolic glabridin on LDL oxidation. Toxicol. Ind. Health 25, 321–324 (2009).
pubmed: 19651803
doi: 10.1177/0748233709103034
Eghlima, G., Sanikhani, M., Kheiry, A., Hadian, J. & Aelaei, M. A Survey of genetic diversity of Glycyrrhiza glabra L. some populations using morphological and phytochemical characteristics. J. Plant Prod. Res. 26, 209–226 (2020).
Yu, F. et al. Effect of genotype and environment on five bioactive components of cultivated licorice (Glycyrrhiza uralensis) populations in northern China. Biol. Pharm. Bull. 38, 75–81 (2015).
pubmed: 25744461
doi: 10.1248/bpb.b14-00574
Sheidai, M., Iraj, S., Karamian, R. & Ranjbar, M. Cyto-morphological studies of the genus Glycyrrhiza in Iran. Cytologia 73, 333–339 (2008).
doi: 10.1508/cytologia.73.333
Hayashi, H. et al. Field survey of Glycyrrhiza plants in Central Asia (1) Characterization of G. uralensis, G. glabra and the putative intermediate collected in Kazakhstan. Biol. Pharm. Bull. 26, 867–871 (2003).
pubmed: 12808302
doi: 10.1248/bpb.26.867
Singh, V., Pal, A. & Darokar, M. P. A polyphenolic flavonoid glabridin: Oxidative stress response in multidrug-resistant Staphylococcus aureus. Free Radic. Biol. Med. 87, 48–57 (2015).
pubmed: 26117328
doi: 10.1016/j.freeradbiomed.2015.06.016