Navigating the path of reproducibility in microRNA-based biomarker research with ring trials.
biomarker
microRNA
ring trial
Journal
Clinical chemistry and laboratory medicine
ISSN: 1437-4331
Titre abrégé: Clin Chem Lab Med
Pays: Germany
ID NLM: 9806306
Informations de publication
Date de publication:
16 May 2024
16 May 2024
Historique:
received:
27
04
2024
accepted:
05
05
2024
medline:
15
5
2024
pubmed:
15
5
2024
entrez:
15
5
2024
Statut:
aheadofprint
Résumé
The development of microRNA (miRNA)-based biomarkers has gained significant attention due to their potential diagnostic, prognostic and therapeutic applications. However, the reproducibility of miRNA biomarker research faces unique challenges, primarily due to the influence of pre-analytical and analytical factors. The absence of standardized procedures contributes to inconsistencies across studies, alongside challenges in reference gene selection, data analysis methods and miRNA profiling platforms. Inter-laboratory comparison trials, or ring trials, offer a strategic approach to address technical and biological variability in miRNA biomarker studies. These trials promote standardization, identify sources of variability and strengthen the correlation between miRNAs and clinical outcomes. Despite their underutilization in miRNA biomarker research, ring trials represent a valuable tool for enhancing reproducibility and expediting the translation of miRNA-based biomarkers into clinical applications.
Identifiants
pubmed: 38747410
pii: cclm-2024-0531
doi: 10.1515/cclm-2024-0531
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 Walter de Gruyter GmbH, Berlin/Boston.
Références
Blanco-Domínguez, R, Sánchez-Díaz, R, de la Fuente, H, Jiménez-Borreguero, LJ, Matesanz-Marín, A, Relaño, M, et al.. A novel circulating microRNA for the detection of acute myocarditis. N Engl J Med 2021;384:2014–27. https://doi.org/10.1056/nejmoa2003608 .
doi: 10.1056/nejmoa2003608
Lakkisto, P, Dalgaard, LT, Belmonte, T, Pinto-Sietsma, S-J, Devaux, Y, de Gonzalo-Calvo, D, et al.. Development of circulating microRNA-based biomarkers for medical decision-making: a friendly reminder of what should NOT be done. Crit Rev Clin Lab Sci 2023;60:141–52. https://doi.org/10.1080/10408363.2022.2128030 .
doi: 10.1080/10408363.2022.2128030
de Gonzalo-Calvo, D, Pérez-Boza, J, Curado, J, Devaux, Y, EU-CardioRNA COST Action CA17129 . Challenges of microRNA-based biomarkers in clinical application for cardiovascular diseases. Clin Transl Med 2022;12:e585. https://doi.org/10.1002/ctm2.585 .
doi: 10.1002/ctm2.585
Orrú, CD, Groveman, BR, Foutz, A, Bongianni, M, Cardone, F, McKenzie, N, et al.. Ring trial of 2nd generation RT-QuIC diagnostic tests for sporadic CJD. Ann Clin Transl Neurol 2020;7:2262–71. https://doi.org/10.1002/acn3.51219 .
doi: 10.1002/acn3.51219
Pisapia, P, Malapelle, U, Roma, G, Saddar, S, Zheng, Q, Pepe, F, et al.. Consistency and reproducibility of next-generation sequencing in cytopathology: a second worldwide ring trial study on improved cytological molecular reference specimens. Cancer Cytopathol 2019;127:285–96. https://doi.org/10.1002/cncy.22134 .
doi: 10.1002/cncy.22134
Junier, T, Huber, M, Schmutz, S, Kufner, V, Zagordi, O, Neuenschwander, S, et al.. Viral metagenomics in the clinical realm: lessons learned from a Swiss-wide ring trial. Genes 2019;10:655. https://doi.org/10.3390/genes10090655 .
doi: 10.3390/genes10090655
McGuire, LI, Poleggi, A, Poggiolini, I, Suardi, S, Grznarova, K, Shi, S, et al.. Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: an international study. Ann Neurol 2016;80:160–5. https://doi.org/10.1002/ana.24679 .
doi: 10.1002/ana.24679
Sopic, M, Kararigas, G, Devaux, Y, Magni, P. Call for participation in the AtheroNET COST Action to implement multiomics in atherosclerotic cardiovascular disease research. Eur Heart J 2023;44:2143–5. https://doi.org/10.1093/eurheartj/ehad153 .
doi: 10.1093/eurheartj/ehad153