DNA Sizing with pg/μL Sensitivity for Cell-Free DNA Analysis with the μLAS Technology.
Capillary
DNA
Microfluidics
Separation
Sizing
cfDNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
16
5
2024
pubmed:
16
5
2024
entrez:
16
5
2024
Statut:
ppublish
Résumé
The μLAS technology enables in-line DNA concentration and separation in a microchannel. Here, we describe its operation to analyze the size profile of cell-free DNA (cfDNA) extracted from blood plasma. Operated on commercial systems for capillary electrophoresis, we provide the size distribution of healthy individuals or patients using an input of 10 μL.
Identifiants
pubmed: 38753139
doi: 10.1007/978-1-0716-3850-7_2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-64Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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