Structures of the mumps virus polymerase complex via cryo-electron microscopy.

Cryoelectron Microscopy Mumps virus / genetics Viral Proteins / metabolism Models, Molecular RNA, Viral / metabolism DNA-Directed RNA Polymerases / metabolism Protein Domains Phosphoproteins / metabolism RNA-Dependent RNA Polymerase / metabolism Virus Replication Transcription, Genetic Protein Conformation

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
17 May 2024
Historique:
received: 24 06 2023
accepted: 26 04 2024
medline: 18 5 2024
pubmed: 18 5 2024
entrez: 17 5 2024
Statut: epublish

Résumé

The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), while structures corresponding to these activities remain obscure. Here, we resolved two L-P complex conformations from the mumps virus (MuV), a typical member of nsNSVs, via cryogenic-electron microscopy. One conformation presents all five domains of L forming a continuous RNA tunnel to the methyltransferase domain (MTase), preferably as a transcription state. The other conformation has the appendage averaged out, which is inaccessible to MTase. In both conformations, parallel P tetramers are revealed around MuV L, which, together with structures of other nsNSVs, demonstrates the diverse origins of the L-binding X domain of P. Our study links varying structures of nsNSV polymerase complexes with genome replication and transcription and points to a sliding model for polymerase complexes to advance along the RNA templates.

Identifiants

DOI: 10.1038/s41467-024-48389-9 PMID: 38760379
pubmed: 38760379
doi: 10.1038/s41467-024-48389-9
pii: 10.1038/s41467-024-48389-9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4189

Subventions

Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 32241028

Informations de copyright

© 2024. The Author(s).

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Auteurs

Tianhao Li (T)

School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China.
School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Mingdong Liu (M)

School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China.

Zhanxi Gu (Z)

University of Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Synthetic Biology, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

Xin Su (X)

School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China.
State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.
ORCID: 0000-0002-5127-7275

Yunhui Liu (Y)

School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China.

Jinzhong Lin (J)

State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.
ORCID: 0000-0001-5666-8526

Yu Zhang (Y)

Key Laboratory of Synthetic Biology, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
ORCID: 0000-0002-1778-8389

Qing-Tao Shen (QT)

School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China. shenqt@sustech.edu.cn.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. shenqt@sustech.edu.cn.
Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China. shenqt@sustech.edu.cn.
School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. shenqt@sustech.edu.cn.
ORCID: 0000-0003-1672-6300

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Structures of the mumps virus polymerase...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus › Virus des oreillons › Structures of the mumps virus polymerase...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Structures of the mumps virus polymerase...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structures of the mumps virus polymerase...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Structures of the mumps virus polymerase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › Structures of the mumps virus polymerase...
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