Serine 39 in the GTP-binding domain of Drp1 is involved in shaping mitochondrial morphology.
Drp1
S39A
mitochondrial network collapse
single amino acid mutagenesis
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
17 May 2024
17 May 2024
Historique:
revised:
18
04
2024
received:
09
01
2024
accepted:
08
05
2024
medline:
18
5
2024
pubmed:
18
5
2024
entrez:
18
5
2024
Statut:
aheadofprint
Résumé
Continuous fusion and fission are critical for mitochondrial health. In this study, we further characterize the role played by dynamin-related protein 1 (Drp1) in mitochondrial fission. We show that a single amino acid change in Drp1 at position 39 from serine to alanine (S39A) within the GTP-binding (GTPase) domain results in a fused mitochondrial network in human SH-SY5Y neuroblastoma cells. Interestingly, the phosphorylation of Ser-616 and Ser-637 of Drp1 remains unaffected by the S39A mutation, and mitochondrial bioenergetic profile and cell viability in the S39A mutant were comparable to those observed in the control. This leads us to propose that the serine 39 residue of Drp1 plays a crucial role in mitochondrial distribution through its involvement in the GTPase activity. Furthermore, this amino acid mutation leads to structural anomalies in the mitochondrial network. Taken together, our results contribute to a better understanding of the function of the Drp1 protein.
Identifiants
pubmed: 38760979
doi: 10.1002/2211-5463.13820
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Általános Orvostudományi Kar, Debreceni Egyetem
ID : 1G3D BKJ0 BFTK 247
Organisme : Debrecen Venture Catapult Program
ID : EFOP-3.6.1-16-2016-00022
Organisme : International Education Office, University of Debrecen, Debrecen, Hungary
Organisme : Tempus Közalapítvány
Organisme : The University of Debrecen Program for Scientific Publication
Informations de copyright
© 2024 The Author(s). FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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