In planta imaging of pyridine nucleotides using second-generation fluorescent protein biosensors.
NADH
NADPH
plastid
pollen tube
ratiometric biosensor
redox
root hair
technical advance
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
18 May 2024
18 May 2024
Historique:
revised:
11
04
2024
received:
26
01
2024
accepted:
24
04
2024
medline:
18
5
2024
pubmed:
18
5
2024
entrez:
18
5
2024
Statut:
aheadofprint
Résumé
Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent biosensors are considered to be the best option to bridge the existing technology gap, as they provide a fast, accurate, and real-time readout. However, the existing pyridine nucleotides genetically encoded fluorescent biosensors are either sensitive to pH change or slow in dissociation rate. Herein, we employed the biosensors which generate readouts that are pH stable for in planta measurement of NADH/NAD
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Hong Kong Research Grants Council General Research Fund
ID : 17102322
Organisme : Hong Kong Research Grants Council General Research Fund
ID : 17103921
Organisme : Agence Nationale de la Recherche
ID : ANR-20-CE13-0025
Organisme : National Natural Science Foundation of China
ID : 31870212
Organisme : National Natural Science Foundation of China
ID : 32070394
Organisme : Hong Kong RGC Postdoctoral Fellowship Scheme
ID : PDFS2324-7S01
Organisme : Human Frontier Science Program
ID : HFSP-RGP 0009/2018
Organisme : Innovation and Technology Fund (Funding Support to State Key Laboratory of Agrobiotechnology)
Organisme : Centre National de la Recherche Scientifique
ID : CNRS (10.13039/501100004794)
Organisme : Area of Excellence Scheme
ID : AoE/M-403/16
Informations de copyright
© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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